The microcirculation

The microcirculation is responsible for the transfer of oxygen from the red blood cells in the capillaries to the cells to meet cellular energy requirements, support functional activity and remove carbon dioxide and waste. The microcirculation also assists in the regulation of vascular tone, solute exchange, the production of hormones, the inflammatory response and haemostasis. The identification of the endothelial glycocalyx and the assessment of the effects of disease, drugs and fluids on the microcirculation is the subject of ongoing research.

Renal replacement

There are few, if any, technological advancements in the field of medicine that have been able to transform a life-threatening condition, in this case, end-stage renal failure, from a certain and horrible death, just some 100 years ago, to a condition manageable within the confines of one’s home. This refresher course, by no means a comprehensive text on peritoneal or haemodialysis, aims to provide the reader (a pre-part one FCA candidate) with the following brief overview: * A short history of dialysis * The basic physiology of fluid and solute exchange employed in renal replacement therapy (RRT) * The physical principals of RRT * Modality

Spatially Resolved STD-NMR Applied to the Study of Solute Transport in Biphasic Systems: Application to Protein-Ligand Interactions

Fluid biphasic systems are one of the most interesting dynamic systems in chemistry and biochemistry. In nuclear magnetic resonance (NMR) spectroscopy, the study of the solute dynamics across fluid biphasic systems requires the introduction of dedicated NMR methods, due to their intrinsic heterogeneity. Diffusion and spatially resolved NMR techniques represent a useful approach for dealing with the study of solutes in biphasic systems and have been applied lately with success. Nevertheless, other potential applications of NMR spectroscopy for biphasic systems remain to be explored. In this proof-of-concept communication, we specifically aimed to investigate whether solute exchange between two immiscible phases can be followed by NMR experiments involving transfer of magnetization. To that aim, we have used spatially resolved saturation transfer difference NMR (SR-STD NMR) experiments to analyze solute exchange by transfer of saturation from one phase to the other in a biphasic system and have explored which are the underlying mechanisms leading to the transfer of magnetization between phases and the limits of the approach. We hereby demonstrate that SR-STD NMR is feasible and that it might be implemented in pharmacological screening for binders of biological receptors or in the study of chemical and biochemical reactions occurring at interfaces.

A time-dependent multi-layered mathematical model of filtration and solute exchange, the revised Starling principle and the Landis experiments

Cell oxygenation and nutrition is vitally important for human and animal life. Oxygen and nutrients are transported by the blood stream and cross microvessel walls to penetrate the cell’s membrane. Pathological alterations in the transport of oxygen, and other nutrition elements, across microvessel walls may have serious consequences to cell life, possibly leading to localized cell necrosis. We present a transient model of plasma filtration and solute transport across microvessel walls by coupling flow and transport equations, the latter being non-linear in solute concentration. The microvessel wall is modeled through the superimposition of two or more membranes with different physical properties, representing key structural elements. With this model, the combined effect of the endothelial cells, the glycocalyx and other coating membranes specific of certain microvessels, can be analyzed. We investigate the role of transient external pressures in the study of trans-vascular filtration and solute exchange during the drop of blood capillary pressure due to the pathological decrease of blood volume called hypovolaemia, as well as hemorrhage. We discuss the advantage of using a multi-layered model, rather than a model considering the microvessel wall as a single and homogeneous membrane.