Prevalence of mycotic agents isolated from skin lesions of trade horses in Obollor-Afor, Enugu State, Nigeria

The study was aimed at identifying mycotic agents that colonize skin lesions in trade horses found in south eastern Nigeria. Skin scrapings were collected from seventy (70) horses with skin lesions in Obollo -Afor market, Enugu state, south eastern Nigeria. Portions of the skin specimen were treated with 10% KOH for microscopic identification of typical hyphae. Sabouraud dextrose agar (SDA) slants, supplemented with cycloheximide were used as a standard substrate for the cultures. Cultures were incubated aerobically for 2 weeks at 37°C and were observed daily for growth of fungi isolates. Identification of fungal species was done based on their cultural and morphological characteristics. From the seventy (70) skin scraping samples studied, fifty-six (56) species of fungi belonging to 6 genera were recovered in different frequencies including Aspergillus sp (54%), Mucor spp (32%), Rhizopus oryzae (7%), Penicilium marneffi (2%), Microsporum fulvum (2%) and Tricophyton equinium (4%). More of the isolates were from the female horses than male horses. At p > 0.05 there was no significant difference in the distribution of fungal isolates between females and male horses. The season of the year had no notable impact on the occurrence or frequency of isolation of the fungi. The isolated dermatophytes may be possible aetiological agents of dermatomycoses in horses, while the Saprobes isolated may be contaminants associated with skin infections in horses. These mycotic agents isolated are not known to primarily, affect humans however there may be a possibility of transmission to human and other susceptible animals that cohabit with these horses. Keywords: Equine, Mycotic agent, Prevalence, Skin lesion

Influencia de herbicidas preemergentes sobre el desarrollo de dermatofitos geofílicos en suelos agrícolas

El objetivo de este trabajo es conocer la acción ejercida por concentraciones crecientes de herbicidas de presiembra (Metrlbuzin, .Atrazina y Alachlor) sobre el crecimiento y la capacidad germinativa de Microsporum fulvum, Microsporum gypseum y Keratinomyces ajelloi

Queratinolisis por microsporum fulvum. Uriburu

Se determina la actividad queratinolitica de cepas de microsporum fulvum aisladas de suelos de praderas, cuando se desarrollan sobre um substrato queratinoso, con y sin aplicación de los herbicidas preemergentes

Influencia de herbicidas sobre la micota queratinolítica de los suelos

Para observar los efectos de ciertos agroquímicos sobre la micota quetatinolítica de los suelos, se realizaron aplicaciones de Metribuzin, Atrazina y Alachlor, en las dosis habituales, a parcelas de terreno de suelos de pradera.Se estudiaron las modificaciones que a través del tiempo se producían en los aislamientos de estos hongos geofílicos. Algunas de las cepas fueron inhibidas al comienzo del tratamiento, pero al cabo de los 60 días, todas ellas se recuperaron a partir de los suelos tratados. Se comprobó que estos agroquímicos produjeron cambios en el metabolismo de la especie más aislada: Microsporum fulvum, atribuyéndose estas observaciones a una selección realizada por los herbicidas, de cepas más resistentes a los mismos

Assessment of Phytochemical Constituents and Antimicrobial Activity of Lantana Camara L.

ABSTRACT: In present study the phytochemical constituents such as total phenol, total flavonoid contents and antibacterial activity against four gram negative and two gram positive isolates Escherichia coli, Pseudomonas aeruginosa, Enterobacter aerogens, Proteus vulgaris, Lactobacillus, S. aureus, Bacillus subtilis and antifungal activity against four fungal strains Penicillium, Aspergillus niger, T. mentagrophytes, Microsporum fulvum of petroleum ether, diethyl ether, chloroform and acetone extract of leaves and flowers of Lantana camara L were evaluated. Maximum zone of inhibition was recorded in the presence of free flavanoid fraction of the plant extract against Trichophyton mentagophytes and Microsporum fulvum which was the most susceptible fungus for all the extracts tested. The extract also compared favourably with streptomycin which serves as a positive control. Minimum inhibitory concentration (MIC) was recorded for all bacteria and fungi in which highest MIC was of B. subtilis and M. fulvum. The UV-Vis and FTIR spectroscopic analysis also revealed the presence of different active groups and bonds. L. camara contains phytochemical compounds with antibacterial & antifungal activities. Moreover, the chloroform & acetone leaf & flower extracts of L. camara are active against pathogenic microorganisms.