Impact of Imazamox and Imazapyr Carryover on Wheat, Barley, and Oat

Imazapyr and imazamox are frequently applied postemergence to control grass and broadleaf weeds in imidazolinone-resistant sunflower in Argentina. Herbicide carryover to rotational crops represents a disadvantage of these herbicides, particularly in regions with low rainfall during the months prior to rotational crop sowing. Between 2009 and 2012, field and greenhouse studies were conducted on four important sunflower-cropped areas of Argentina. The objective was to quantify the effects of imazapyr alone and imazamox plus imazapyr applied in sunflower crops on the subsequent establishment, growth, and yield of barley, oat, and wheat. In all field experiments, imazapyr alone and imazamox plus imazapyr were applied at recommended rates (80 gha–1and 66 plus 30 gha–1, respectively), and also, in some experiments, at double the recommended rates. Soil bioassays were also conducted in the greenhouse to study the effect of these herbicides on barley, oat, and wheat seedlings. The mixture of imazamox plus imazapyr was safer for rotational crops than imazapyr applied alone, because of the reduced rate of imazapyr in the mixture treatments. Barley was more sensitive to imidazolinones, particularly imazapyr, than the other winter cereals. Imazapyr at double rate (160 gha–1) reduced barley yield by 45% when seeds were sown 165 d after herbicide application and with 240 mm rainfall after herbicide application.

Development of a Soil Bioassay for Triclopyr Residues and Comparison with a Laboratory Extraction

The use of triclopyr for the removal of woody and broad-leaf vegetation in right-of-ways and agricultural settings has been proposed for Alaska. Triclopyr concentrations in soil after application are of concern because residual herbicide may affect growth of subsequent vegetation. In order to measure triclopyr residues in soil and determine the amount of herbicide taken up by the plant, soil bioassays were developed. Four agricultural species, turnip, lettuce, mustard, and radish, were tested to determine sensitivity to triclopyr in a 1-wk bioassay. The sensitivity (I50) of turnip, lettuce, mustard, and radish was 0.33 ± 0.05 kg ai ha−1, 0.78 ± 0.11 kg ai ha−1, 0.78 ± 0.07 kg ai ha−1, and 0.85 ± 0.10 kg ai ha−1 (mean ± SE), respectively. Mustard was the most consistent crop in the bioassay with a midrange response to triclopyr and lowest standard deviation for germination as compared to the other species. Thus, it was used in a bioassay to determine triclopyr concentrations in a field trial. The bioassay of mustard closely matched residual amounts of triclopyr in a field trial determined by chemical extraction. Estimates of residual triclopyr concentrations using the bioassay method were sometimes less than the triclopyr concentration determined using a chemical extraction. These differences in concentrations were most evident after spring thaw when the chemical extraction determined there was enough triclopyr in the soil to reduce mustard growth over 60%, yet the bioassay measured only a 10% reduction. The chemical extraction method may have identified nonphototoxic metabolites of triclopyr to be the herbicidal triclopyr acid. These methods, when analyzed together with a dose–response curve, offer a more complete picture of triclopyr residues and the potential for carryover injury to other plant species.

Pretransplant fertilization of containerized Picea mariana seedlings: calibration and bioassay growth response

The role of the root plug as a nutrient source for newly planted seedlings was evaluated for one growing season on soil bioassays retrieved from a boreal forest site. Intact (control) and bare-rooted (peat plug removed) black spruce (Picea mariana (Mill.) B.S.P.) seedlings reared in Jiffy pellets, some fertilized before ("spiked" with 60 mg N) or after (topdressed with 300 mg N) planting, were transplanted to potted soil blocks (bioassays) under greenhouse conditions. Compared with the intact control, bare-rooting alone reduced plant dry mass (16%) and N, P, and K (15%–25%) uptake, but increased these parameters (62%–101%) when combined with topdressing, suggesting that the root plug served as a crucial nutrient reserve soon after transplanting. Nutrient spiking or topdressing alone stimulated growth and nutrient uptake as well (35%–118%), but generated the largest response (81%–205%) when applied together. Mortality (7%–18%) occurred only with bare-rooting treatments. The responses reflected the sensitivity of seedlings to nutrient supply changes both in root plugs and in field soils. Nutrient spiking was more efficient in improving seedling performance than traditional topdressing because of reduced fertilizer requirements and closer availability of added nutrients for early root development.

Chemical and biological induction of resistance to the clover cyst nematode (Heterodera trifolii) in white clover (Trifolium repens)

Possible induction of resistance to the clover cyst nematode, Heterodera trifolii, in white clover, Trifolium repens, by application of two chemical inducers and of soil-borne Pseudomonas-like spp. and Bacillus cereus was investigated. Salicylic acid and benzo(1,2,3)thiadiazole-7-carbothioic acid-S-methyl ester, applied as a root drench in growth cabinet soil bioassays, affected development of H. trifolii in cvs Haifa and Grasslands Huia. Treatments reduced fecundity of the nematodes, increasing the proportions of distorted females and of females with fewer eggs compared to water-treated controls. Application of pectinolytic fluorescent pseudomonad strains P29 and P80, and B. cereus strain B1, induced a response similar to that resulting from the chemical induction. Both live and dead cells of P29, but not cell-free culture filtrate, induced these effects on H. trifolii. A non-pectinolytic, fluorescent pseudomonad, strain P37, had no effect. From the nature of the responses, it is concluded that the effects of both the chemical and bacterial agents have similarities to resistance. From the timing of applications and known properties of both agents, these effects seem similar to induced systemic resistance.

Duration of Broadleaf Weed Control with Isoxaben Using Soil Bioassays

A soil bioassay experiment was conducted in Blacksburg, VA, to determine the effect of isoxaben application timings and rates on duration of weed control. Flats containing soil were imbedded into the field. Isoxaben was applied in the spring, fall, and spring followed by fall (double application) at 0.56, 0.84, and 1.12 kg ai/ha. Flats were moved to a greenhouse at 0, 1, 3, 6, and 9 months after treatment (MAT) and seeded with yellow rocket, buckhorn plantain, and spotted spurge for the bioassays. Weed counts from treated flats were compared to those in untreated flats to determine percent control. Fall and spring followed by fall applications provided approximately 20% greater control of yellow rocket 3 MAT and 30% greater control 6 MAT, compared to a single spring application of isoxaben. Isoxaben at all rates controlled yellow rocket > 70% at 6 mo after fall application. At 3 MAT, fall and spring plus fall-applied isoxaben provided about 15 and 20% greater buckhorn plantain control, respectively, compared to spring application. The two highest rates of isoxaben controlled buckhorn plantain > 70% at 3 mo after fall application, but provided poor control at 6 MAT. Control of spotted spurge was similar among the three application timings for isoxaben and was unacceptable at most evaluation dates. Overall, isoxaben applied at 1.12 kg/ha provided better control of all three weed species for a longer time than the reference herbicide, oxadiazon, applied at 3.36 kg/ha.

Characterization of red alder ectomycorrhizae: a preface to monitoring belowground ecological responses

Critical ecological research on belowground ecosystems has often been impeded because of the inability to adequately recognize ectomycorrhizal relationships, especially the abundance, diversity, and distribution of the fungus component, and the specificity of particular fungus–host combinations. Red alder, with its high degree of host specificity and paucity of fungal symbionts, provides an ideal model for studying these attributes. Eleven morphologically recognizable types of ectomycorrhizae were characterized from field-collected root material, greenhouse soil bioassays, and laboratory syntheses. Most mycobionts were basidiomycetes, as evidenced by abundant clamp connections present in the mantle and extramatrical hyphae. Seven mycobionts identified to species included Alpova diplophloeus, Thelephora terrestris, Lactarius obscuratus, Cortinarius bibulus, Laccaria laccata, Hebeloma crustuliniforme, and Paxillus involutus. Many of the ectomycorrhizae collected in the field appeared to have more than one mycobiont present in the mantle. Root tips could generally be categorized into either flexuous or succulent morphological types. The flexuous types were long, thin, indeterminate in growth, with an acute root apex, and the mantle and Hartig net in longitudinal section were not well formed near the root apex. The succulent types were short, thick, determinate in growth, with a rounded root apex, and the mantle and Hartig net in longitudinal section were well formed near the root apex. Additional characteristics important in distinguishing among red alder ectomycorrhizal types included color, extent of extramatrical hyphae development, mantle surface characteristics, and selected microchemical reactions. Mantle thickness was highly variable and not useful in characterization. Hartig net development was shallow, and regardless of mycorrhizal origin, rarely extended beyond one epidermal cell layer. Key words: ectomycorrhizae, Alnus, characterization, ecology, belowground.