Oxidative stress and biochemical indicators in blood of patients addicted to alcohol treated for acute ethylene glycol poisoning

Ethylene glycol (EG), in addition to its neurotoxic and nephrotoxic effects, evokes oxidative stress. The aim of this study was to assess the influence of the ethylene glycol on the biochemical indicators and oxidoreductive balance of patients treated for acute poisoning. The total study group consisted of 56 persons including 26 alcoholics who took EG as a substitute for ethyl alcohol in the course of alcohol dependence syndrome and 30 controls. Severity of poisoning, results of acid-base parameters, biochemical, and toxicological tests as well as biomarkers of the oxidative stress in blood were analyzed during the patients’ hospitalization. The key issue was to assess the oxidative stress and biochemical disturbances caused by EG and the type of treatment applied in the course of poisoning. Significant changes in some parameters were found both at time of diagnosis and after treatment initiation (ethanol as an antidote and hemodialysis). The most important differences included the activity of hepatic parameters (aspartate aminotransferase, AST) and oxidative stress markers like catalase (CAT); correlation of the lipid peroxidation products level (TBARS) with urea concentration has been shown. On the last day of the hospitalization, in some cases, the mutual correlation between the evaluated markers were observed, for example, between alanine transaminase (ALT) and glutathione reductase (GR), and urea concentration and glutathione level (GSH/GSSG). The concentration of ions (H+) had a major impact on the oxidoreductive balance, correlating with the elevated GR and GSH/GSSG levels.

Testing Tool Material on Scratch Tester

In manufacturing industry cutting tools are considered as the backbone of the metal cutting operation. In metal cutting operation there is relative motion between the tool and the workpiece. As the tool material is harder than the workpiece material, there is deformation of the workpiece which acts as a base for the formation of chips. If we observe the process of metal cutting, we can easily find out that there is a considerable amount of heat generated during the machining operation. As there is a point of interface between the tool and the workpiece, there is absorption of generated heat into both the tool as well as work material. Due to the absorption of the heat there is distortion in the tool material. In this research article we have taken the base parameters as speed, load and stroke and the output parameter is taken as the load which breaks the coating of the tool. Keywords: tool coating, scratch tester, speed, stroke, coating.

Modeling and base parameters identification of legged robots

This paper first uses a decoupling modeling method to model legged robots. The method groups all the degrees of freedom according to the number of limbs, regarding each limb as a manipulator with serial structure, which greatly reduces the number of dynamic parameters that need to be identified simultaneously. On this basis, a step-by-step identification method from the end-effector link to the base link, referred to as “E-B” identification method, is proposed. Simulation verification is carried out on a quadruped robot with 16 degrees of freedom in Gazebo, and the validity of the method proposed is discussed.

Treatment of Metabolic Alkalosis with Rice Broth

The available treatments of metabolic alkalosis should be given in the hospital and may be associated with serious adverse events. We aimed to use rice broth for treatment of metabolic alkalosis. Patients with metabolic alkalosis were received oral rice broth solution; serum electrolytes and acid-base parameters were measured before and after the treatment. The majority of patients were older and in the ICU. After treatment with rice broth solution, there was statistical significant decreased in blood PH and serum [HCO3-] from a mean of 7.45 ± 0.03 to 7.4 ± 0.009 and 34.5 ± 4.4 to 24.9 ± 1.1 mmol/l respectively and statistical significant increase in serum [Na+], serum [Cl-], serum [K+], serum [Ca+2], serum [PO4-2] and serum [Mg+2] from a mean of 132.5 ± 3.7 to 136.5 ± 1.9 mmol/l, 93.2 ± 5.4 to 101.9 ± 2.7 mmol/l, 3.1 ± 0.3 to 3.6 ± 0.2 mmol/l, 0.99 ± 0.1 to 1 ± 0.1mmol/l, 3.4 ± 0.5 to 3.6 ± 0.4 mg/dl and 1.88 ± 0.2 to 2.28 ± 0.17 mg/dl respectively. Rice broth is an effective oral treatment of metabolic alkalosis.

Electrolytes, Metabolic And Acid-Base Parameters In Blood And Fluids of The Reproductive Tract During In Vivo Maturation of Bovine Oocytes

Follicular fluid is the microenvironment that supports oocyte maturation and competence. Using Abbott iSTAT1™ and NanoDrop, we determined the dynamics of acid-base, electrolyte, metabolites, and total protein in venous blood, fluids of the dominant follicle (FF), oviduct (OF), and uterus (UF) during the window of oocyte maturation. Holstein heifers (n=36) were synchronized with PGF2α on Days -11 and 0, CIDR during Days -6 to 1, and GnRH given on Day 2 after 2nd PG. Samples were collected at 24h, 48h, 60h, 72h, and 78h after 2nd PG. Most electrolytes analyzed, Cl-, K+, and Ca2+ were significantly affected in blood and FF (P<0.05) by CIDR removal. Similarly, Cl- and Na+ also significantly changed in OF and UF across time. Glucose, lactate, and creatinine significantly changed across time points in FF compared to blood. Moreover, pO2, pCO2, TCO2, and pH significantly changed across time in FF. Most parameters were not significantly correlated between blood and FF across time points except for glucose, Cl- and creatinine. Furthermore, pO2 in FF was nearly 3X higher than blood, suggesting low O2 during in vitro maturation is inappropriate. In conclusion, components of the follicular fluid undergo major changes during the window of oocyte maturation.

STUDY OF ACID BASE BALANCE IN CHRONIC SEVERE ANAEMIA

Aim of the study role of acid base balance in chronic severe anaemic patients admitted in Department of Medicine, Darbhanga Medical College and Hospital, Laheriasarai, Bihar. Study subject include 50 chronic and severe anaemic patients. All the fty cases underwent detailed haematological, biochemical, electrocardiographic, roentgenographic studies followed by extensive analysis of blood for blood gases and acid base parameters at the onset. Some were further studied for venous blood lactate and the effect of intravenous frusemide on the acid base parameters and the clinical state of the patient. Twenty normal controls were also studied. Work in future on the problem of chronic severe anaemia should include cases with cardiac failure and other complications like respiratory infections etc. and they should be studied for myocardial function, glomerular ltration rate and renal anion transport in addition to blood gases and acid base study as done by us. The role of ionotropic agents should be studied in such cases in isolation from that with diuretics. The administration of oxygen alone with a mask is also expected to improve almost all parameters quickly and should be studied.