Pretreatment Affects Profits From Xylanase During Enzymatic Saccharification of Corn Stover Through Changing the Interaction Between Lignin and Xylanase Protein

Effective pretreatment is vital to improve the biomass conversion efficiency, which often requires the addition of xylanase as an accessory enzyme to enhance enzymatic saccharification of corn stover. In this study, we investigated the effect of two sophisticated pretreatment methods including ammonium sulfite (AS) and steam explosion (SE) on the xylanase profits involved in enzymatic hydrolysis of corn stover. We further explored the interactions between lignin and xylanase Xyn10A protein. Our results showed that the conversion rates of glucan and xylan in corn stover by AS pretreatment were higher by Xyn10A supplementation than that by SE pretreatment. Compared with the lignin from SE pretreated corn stover, the lignin from AS pretreated corn stover had a lower Xyn10A initial adsorption velocity (13.56 vs. 10.89 mg g−1 min−1) and adsorption capacity (49.46 vs. 27.42 mg g−1 of lignin) and weakened binding strength (310.6 vs. 215.9 L g−1). Our study demonstrated the low absolute zeta potential and strong hydrophilicity of the lignin may partly account for relative weak interaction between xylanase protein and lignin from AS pretreated corn stover. In conclusion, our results suggested that AS pretreatment weakened the inhibition of lignin to enzyme, promoted the enzymatic hydrolysis of corn stover, and decreased the cost of enzyme in bioconversion.

Production of ammonia makes Venusian clouds habitable and explains observed cloud-level chemical anomalies

The atmosphere of Venus remains mysterious, with many outstanding chemical connundra. These include the unexpected presence of ∼10 ppm O2 in the cloud layers, an unknown composition of large particles in the lower cloud layers, and hard to explain measured vertical abundance profiles of SO2 and H2O. We propose a hypothesis for the chemistry in the clouds that largely addresses all of the above anomalies. We include ammonia (NH3), a key component that has been tentatively detected both by the Venera 8 and Pioneer Venus probes. NH3 dissolves in some of the sulfuric acid cloud droplets, effectively neutralizing the acid and trapping dissolved SO2 as ammonium sulfite salts. This trapping of SO2 in the clouds, together with the release of SO2 below the clouds as the droplets settle out to higher temperatures, explains the vertical SO2 abundance anomaly. A consequence of the presence of NH3 is that some Venus cloud droplets must be semisolid ammonium salt slurries, with a pH of ∼1, which matches Earth acidophile environments, rather than concentrated sulfuric acid. The source of NH3 is unknown but could involve biological production; if so, then the most energy-efficient NH3-producing reaction also creates O2, explaining the detection of O2 in the cloud layers. Our model therefore predicts that the clouds are more habitable than previously thought, and may be inhabited. Unlike prior atmospheric models, ours does not require forced chemical constraints to match the data. Our hypothesis, guided by existing observations, can be tested by new Venus in situ measurements.

Pretreatment of Bamboo by Ammonium Sulfite for Efficient Enzymatic Hydrolysis

In order to find out the potential effect of ammonium sulfite pretreatment on bamboo for efficient enzymatic saccharification, various ammonium sulfite loadings were conducted to pretreat bamboo at 180 °C for 1–8 h with the solid–liquid ratio of 1:6, respectively. The results verified that ammonium sulfite pretreatment could effectively disrupt the recalcitrance of bamboo and create highly enzymatic hydrolysis sugar yield for further utilization. The contents of lignin in the substrates decreased significantly (from 22.94% to 1.21%) after pretreated and the higher lignin removal with higher chemical loading and longer reaction time during pretreatment. The cellulose-to-glucose conversion yield of pretreated bamboo substrates could achieve almost 100% within 48 h by enzymes loading of 15 FPU of cellulase and 30 IU of β-glucosidase per gram glucan. Therefore, ammonium sulfite pretreatment is argued to be a potential alternative technology for pretreatment of bamboo.

Impact of ammonium sulfite-based sequential pretreatment combinations on two distinct saccharifications of wheat straw

The impact of substrate properties on wheat straw sugar release from fungal cellulase and whole cell-based CBS was comparatively investigated.