translocation velocity
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2020 ◽  
Author(s):  
Chaoyou Xue ◽  
Lucia Molnarova ◽  
Justin B Steinfeld ◽  
Weixing Zhao ◽  
Chujian Ma ◽  
...  

Abstract RECQ5 is one of five RecQ helicases found in humans and is thought to participate in homologous DNA recombination by acting as a negative regulator of the recombinase protein RAD51. Here, we use kinetic and single molecule imaging methods to monitor RECQ5 behavior on various nucleoprotein complexes. Our data demonstrate that RECQ5 can act as an ATP-dependent single-stranded DNA (ssDNA) motor protein and can translocate on ssDNA that is bound by replication protein A (RPA). RECQ5 can also translocate on RAD51-coated ssDNA and readily dismantles RAD51–ssDNA filaments. RECQ5 interacts with RAD51 through protein–protein contacts, and disruption of this interface through a RECQ5–F666A mutation reduces translocation velocity by ∼50%. However, RECQ5 readily removes the ATP hydrolysis-deficient mutant RAD51–K133R from ssDNA, suggesting that filament disruption is not coupled to the RAD51 ATP hydrolysis cycle. RECQ5 also readily removes RAD51–I287T, a RAD51 mutant with enhanced ssDNA-binding activity, from ssDNA. Surprisingly, RECQ5 can bind to double-stranded DNA (dsDNA), but it is unable to translocate. Similarly, RECQ5 cannot dismantle RAD51-bound heteroduplex joint molecules. Our results suggest that the roles of RECQ5 in genome maintenance may be regulated in part at the level of substrate specificity.


2019 ◽  
Vol 1080 ◽  
pp. 66-74 ◽  
Author(s):  
Ardalan Ganjizade ◽  
Seyed Nezameddin Ashrafizadeh ◽  
Arman Sadeghi

2019 ◽  
Vol 48 (3) ◽  
pp. 261-266 ◽  
Author(s):  
Han Yan ◽  
Daming Zhou ◽  
Biao Shi ◽  
Ziyin Zhang ◽  
Haibing Tian ◽  
...  

Polymers ◽  
2019 ◽  
Vol 11 (1) ◽  
pp. 118 ◽  
Author(s):  
Sahin Buyukdagli ◽  
Jalal Sarabadani ◽  
Tapio Ala-Nissila

The theoretical formulation of driven polymer translocation through nanopores is complicated by the combination of the pore electrohydrodynamics and the nonequilibrium polymer dynamics originating from the conformational polymer fluctuations. In this review, we discuss the modeling of polymer translocation in the distinct regimes of short and long polymers where these two effects decouple. For the case of short polymers where polymer fluctuations are negligible, we present a stiff polymer model including the details of the electrohydrodynamic forces on the translocating molecule. We first show that the electrohydrodynamic theory can accurately characterize the hydrostatic pressure dependence of the polymer translocation velocity and time in pressure-voltage-driven polymer trapping experiments. Then, we discuss the electrostatic correlation mechanisms responsible for the experimentally observed DNA mobility inversion by added multivalent cations in solid-state pores, and the rapid growth of polymer capture rates by added monovalent salt in α -Hemolysin pores. In the opposite regime of long polymers where polymer fluctuations prevail, we review the iso-flux tension propagation (IFTP) theory, which can characterize the translocation dynamics at the level of single segments. The IFTP theory is valid for a variety of polymer translocation and pulling scenarios. We discuss the predictions of the theory for fully flexible and rodlike pore-driven and end-pulled translocation scenarios, where exact analytic results can be derived for the scaling of the translocation time with chain length and driving force.


2018 ◽  
Vol 165 (4) ◽  
pp. 353-359
Author(s):  
Youské Shimizu ◽  
Toru Togawa ◽  
Shigeru Chaen

Abstract The amino acid sequence of the motor domain of AnKinA, kinesin-1 from Aspergillus nidulans, growing optimally at 37°C, was compared with that of SbKin1, kinesin-1 from the snow mold Sclerotinia borealis. For cold-adaptation, some enzymes are thought to exhibit augmented protein structure flexibility, acquired most effectively by substituting a glycine residue for another amino acid residue. By the comparison described above, two glycine residues proximal to tightly bound ADP were identified in the SbKin1 motor domain, of which the corresponding residues of AnKinA were non-glycine ones (P60 and S323). We made AnKinA recombinant kinesin (AnKinA-WT (WT)) along with P60G and S323G mutants. From the basal ATPase activity (without microtubules), these kinesins showed similar characteristics in activation energies, while deviation from the linearity of the ATPase activity time-course was detected at 34°C for WT and P60G but at 24°C for S323G. The microtubule translocation velocity of WT, P60G or S323G exhibited an activation energy of 60, 58 or 53 kJ/mol, respectively; for S323G, the activation energy was lower and the velocity at low temperatures was higher than those for the other two. These results suggest that the point mutation S323G would offer possible cold-adaptation in compensation for thermal stability.


2017 ◽  
Vol 114 (15) ◽  
pp. E2991-E2997 ◽  
Author(s):  
Antonio Suma ◽  
Cristian Micheletti

We use an accurate coarse-grained model for DNA and stochastic molecular dynamics simulations to study the pore translocation of 10-kbp–long DNA rings that are knotted. By monitoring various topological and physical observables we find that there is not one, as previously assumed, but rather two qualitatively different modes of knot translocation. For both modes the pore obstruction caused by knot passage has a brief duration and typically occurs at a late translocation stage. Both effects are well in agreement with experiments and can be rationalized with a transparent model based on the concurrent tensioning and sliding of the translocating knotted chains. We also observed that the duration of the pore obstruction event is more controlled by the knot translocation velocity than the knot size. These features should advance the interpretation and design of future experiments aimed at probing the spontaneous knotting of biopolymers.


2017 ◽  
Vol 33 (6) ◽  
pp. 735-738 ◽  
Author(s):  
Xiaoyin SUN ◽  
Takao YASUI ◽  
Takeshi YANAGIDA ◽  
Noritada KAJI ◽  
Sakon RAHONG ◽  
...  

2016 ◽  
Vol 2016 ◽  
pp. 1-13 ◽  
Author(s):  
Zewen Liu ◽  
Yifan Wang ◽  
Tao Deng ◽  
Qi Chen

The solid-state nanopore-based DNA sequencing technology is becoming more and more attractive for its brand new future in gene detection field. The challenges that need to be addressed are diverse: the effective methods to detect base-specific signatures, the control of the nanopore’s size and surface properties, and the modulation of translocation velocity and behavior of the DNA molecules. Among these challenges, the realization of the high-quality nanopores with the help of modern micro/nanofabrication technologies is a crucial one. In this paper, typical technologies applied in the field of solid-state nanopore-based DNA sequencing have been reviewed.


Nanoscale ◽  
2016 ◽  
Vol 8 (45) ◽  
pp. 19139-19147 ◽  
Author(s):  
L. J. Mayne ◽  
S. D. R. Christie ◽  
M. Platt

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