scholarly journals Solid-State Nanopore-Based DNA Sequencing Technology

2016 ◽  
Vol 2016 ◽  
pp. 1-13 ◽  
Author(s):  
Zewen Liu ◽  
Yifan Wang ◽  
Tao Deng ◽  
Qi Chen

The solid-state nanopore-based DNA sequencing technology is becoming more and more attractive for its brand new future in gene detection field. The challenges that need to be addressed are diverse: the effective methods to detect base-specific signatures, the control of the nanopore’s size and surface properties, and the modulation of translocation velocity and behavior of the DNA molecules. Among these challenges, the realization of the high-quality nanopores with the help of modern micro/nanofabrication technologies is a crucial one. In this paper, typical technologies applied in the field of solid-state nanopore-based DNA sequencing have been reviewed.

2021 ◽  
pp. 1639-1648
Author(s):  
Yu-Ting Chen ◽  
Marc Duquesnoy ◽  
Darren H. S. Tan ◽  
Jean-Marie Doux ◽  
Hedi Yang ◽  
...  

Author(s):  
Lukasz D. Kaczmarek ◽  
Todd B. Kashdan ◽  
Maciej Behnke ◽  
Martyna Dziekan ◽  
Ewelina Matuła ◽  
...  

AbstractWhen individuals communicate enthusiasm for good events in their partners' lives, they contribute to a high-quality relationship; a phenomenon termed interpersonal capitalization. However, little is known when individuals are more ready to react enthusiastically to the partner's success. To address this gap, we examined whether positive and negative emotions boost or inhibit enthusiastic responses to partner's capitalization attempts (RCA). Participants (N = 224 individuals) responded to their partner's success. Before each capitalization attempt (operationalized as responses following the news that their partner won money in a game), we used video clips to elicit positive (primarily amusement) or negative (primarily anger) or neutral emotions in the responder. We recorded emotional valence, smiling intensity, verbal RCA, and physiological reactivity. We found indirect (but not direct) effects such that eliciting positive emotions boosted and negative emotions inhibited enthusiastic RCA (smiling intensity and enthusiastic verbal RCA). These effects were relatively small and mediated by emotional valence and smiling intensity but not physiological reactivity. The results offer novel evidence that positive emotions fuel the capitalization process.


2021 ◽  
Vol 227 ◽  
pp. 111014
Author(s):  
Chien-Chung Hsu ◽  
Sheng-Min Yu ◽  
Kun-Mu Lee ◽  
Chuan-Jung Lin ◽  
Hao-Chien Cheng ◽  
...  

2021 ◽  
Vol 8 (2) ◽  
pp. 192-199
Author(s):  
Christina Mulcahy ◽  
Crystal A. Day Hess ◽  
Douglas H. Clements ◽  
Jasmine R. Ernst ◽  
Sarah E. Pan ◽  
...  

Early childhood teachers face competing instructional priorities to support specific academic skills and general skills that underlie learning, such as executive function (EF) skills that allow children to control their own thinking and behavior. As the evidence shows, EF skills predict later mathematics achievement, and early mathematics predicts later EF. These relations between mathematics and EF suggest high-quality mathematics teaching has a dual benefit: Teachers can promote children’s math and EF competencies by embedding support for EF in high-quality mathematics activities. Children benefit when guided to reflect on solutions and alternative strategies, and teachers benefit from guidance on how to support both math and EF. Finally, research on teachers developing both domains can inform educational policy.


2021 ◽  
Author(s):  
Jie Wang ◽  
Shiming Li ◽  
Lei Lan ◽  
Mushan Xie ◽  
Shu Cheng ◽  
...  

Abstract Background: Setaria italica is the second-most widely planted species of millets in the world and an important model grain crop for the research of C4 photosynthesis and abiotic stress tolerance. Through three genomes assembly and annotation efforts, all genomes were based on next generation sequencing technology, which limited the genome continuity. Results: Here we report a high-quality whole-genome of new cultivar Huagu11, using single-molecule real-time sequencing and High-throughput chromosome conformation capture (Hi-C) mapping technologies. The total assembly size of the Huagu11 genome was 408.37 Mb with a scaffold N50 size of 45.89 Mb. Compared with the other three reported millet genomes based on the next generation sequencing technology, the Huagu11 genome had the highest genomic continuity. Intraspecies comparison showed about 94.97% and 94.66% of the Yugu1 and Huagu11 genomes, respectively, were able to be aligned as one-to-one blocks with four chromosome inversion. The Huagu11 genome contained approximately 19.43 Mb Presence/absence Variation (PAV) with 627 protein-coding transcripts, while Yugu1 genomes had 20.53 Mb PAV sequences encoding 737 proteins. Overall, 969,596 Single-nucleotide polymorphism (SNPs) and 156,282 insertion-deletion (InDels) were identified between these two genomes. The genome comparison between Huagu11 and Yugu1 should reflect the genetic identity and variation between the cultivars of foxtail millet to a certain extent. The Ser-626-Aln substitution in acetohydroxy acid synthase (AHAS) was found to be relative to the imazethapyr tolerance in Huagu11. Conclusions: A new improved high-quality reference genome sequence of Setaria italica was assembled, and intraspecies genome comparison determined the genetic identity and variation between the cultivars of foxtail millet. Based on the genome sequence, it was found that the Ser-626-Aln substitution in AHAS was responsible for the imazethapyr tolerance in Huagu11. The new improved reference genome of Setaria italica will promote the genic and genomic studies of this species and be beneficial for cultivar improvement.


Plant Disease ◽  
2021 ◽  
Author(s):  
Fengzhi Bie ◽  
Yiming Li ◽  
Zhibin Liu ◽  
Meijing Qin ◽  
Shuping Li ◽  
...  

Xanthomonas citri pv. mangiferaeindicae (Xcm) is the causal agent of mango bacterial black spot which is present in many mango growing regions and leads to great economic losses to mango industry. Due to the limitation of high-quality genomic resources, little is known about the molecular pathogenesis of Xcm. Here, we used PacBio High Fidelity reads (HiFi) sequencing technology to sequence and analyze the whole genome of an Xcm strain GXG07 isolated from Guangxi, the largest mango growing region in China. PacBio HiFi reads with a mean coverage of 450× had been assembled into three contigs of 5,166,537, 79,634 and 30,169 bp, revealing that the genome of Xcm GXG07 contains one chromosome and two plasmids. This genome provides a resource to better understand the biology and pathogenicity of mango bacterial black spot.


2013 ◽  
Vol 2013 (CICMT) ◽  
pp. 000187-000191
Author(s):  
Katsuya Teshima ◽  
Hajime Wagata ◽  
Shuji Oishi

All-solid-state lithium-ion rechargeable batteries (LIBs) consisting of solid electrolyte materials have attracted a number of research interests because no use of organic liquid electrolyte increases packaging density and intrinsic safety of LIB, which contribute the development on environmentally-friendly automobiles such as electric vehicle (EV), hybrid vehicle (HV), and plug-in hybrid vehicle (HEV), in addition to efficient utilization of electric energy in smart grid. Among various solid electrolytes, inorganic electrolyte materials have achieved relatively high lithium-ion conductivity and better stability at an ambient atmosphere. Nevertheless, there is a drawback that is relatively high internal resistance owing to relatively slow Li ion movement caused by low crystallinity of materials, scattering at interfaces such as current collector/electrode active materials and electrode active materials/electrolyte materials. In this context, we have proposed a concept, all-crystal-state LIB, in which all the component materials have high crystallinity and those interfaces are effective for Li ion diffusion. Here, we present the fabrication of oxide crystals and crystal layers via flux method and flux coating. Flux method is one of the solution processes in which idiomorphic highly crystalline materials can be obtained under the melting point of the target ones. In addition, it provides simple, low-cost and environmentally-benign pathway compared to conventional solid-state-reaction method. Flux coating method is developed to fabricate high-quality crystal layers (films) on various substrates. High-quality crystals and crystal layers of cathode, anode and electrolyte materials were successfully fabricated.


2018 ◽  
Vol 51 (02) ◽  
pp. 115-122
Author(s):  
Sanjay Parashar ◽  
Aniketh Venkataram

ABSTRACT Background: Liposuction and abdominal contouring is one of the most common procedures performed by plastic surgeons worldwide. Surprisingly, there has been few attempts at dividing the abdomen into subunits to delineate and aid in this endeavour. We have evolved a system of abdominal aesthetic subunits which has enabled us to achieve high quality results. Materials and Methods: A system of 8 subunits of the abdomen was evolved after analysing 1000 cases: Upper midline, upper rectus, lower rectus, pubic, Lateral abdomen, Lumbar Posterior midline and Bra Roll. Results: The system was used in 2000 cases with good results. The system enabled the performance of tailored liposuction in each area, with greater accuracy, better results and fewer complications. Conclusion: Our success with this classification validates its use, and makes liposuction more scientific and effective.


Sign in / Sign up

Export Citation Format

Share Document