Neutralizing TNT Acidic Wastewater With Neem Leaves Powder

This study conducted to substituting of NaOH used as a neutralizing agent for acidic wastewater generated from manufacturing of TNT in factory of Sudanese Military industries by natural material such as Neem leaves powder. The requirement for pH values of astewater to be disposed over 6. Four weights (5 , 7.5, 10 and 12.5 ) g of dry Neem leaves powder prepared and added to four samples of acidic wastewater with volume of 100 ml for each samples, the pH values of wastewater measured after four periods (1 ,2,3 and 4) hr for each weight added. The results showed the pH values raised from 1.7 to 6.94 and no any residual salts observed. The Neem leaves powder is effective alternatives for sodium hydroxide to neutralizing acidic wastewater at TNT plant.

Mineral Materials as a Neutralizing Agent Used on Soil Contaminated with Copper

The aim of the investigation was to evaluate the response of plants, using black mustard (Brassica nigra L. Koch) as a model plant, to soil contamination with copper (0, 200, 400, 600 mg Cu kg−1 of soil), and to determine the effectiveness of the Cu immobilization with mineral neutralizing materials, such as lime, clay and zeolite. The plant yield depended on soil contamination and mineral amendments. In the series without neutralizing materials, the level of 600 mg Cu kg−1 reduced the yield and increased leaf greenness. Lime alleviated the toxicity of Cu in objects with 200 mg Cu kg−1. Zeolite slightly mitigated the harmful effects of Cu at the level of 400 and 600 mg kg−1. Zeolite lowered the SPAD index. In the chemical composition of plants, the content of Cu, K, Mg, Na and Ca in plants increased to 400 mg Cu kg−1, while the content of P decreased to 600 mg Cu kg−1. Among the materials, lime reduced the Cu accumulation in plants the most, followed by clay. Cu narrowed the majority of ratios and widened the Ca:P and K:Ca ratios in plants. The applied mineral materials, except lime, did not significantly affect the formation of these indicators.

Protamine—The Journey from DNA to Heparin Neutralization to Gene therapy

Protamine is now well recognized as a key heparin neutralizing agent. However, protamine was discovered over a century ago, during experiments performed to uncover the secrets behind heritability. Although protamine was discovered as a highly charged protein, it did not receive the attention it deserved until the dawn of insulin era, when it was used to create the neutral protamine Hagedorn formulation. Based on the same principles, protamine was identified to neutralize heparin and has since been used successfully for many years in cardiothoracic surgery. More recently, its clinical applications have extended to gene therapy. In this historical sketch, the journey from the discovery of protamine, onwards to heparin neutralization, and up to its utilization in genetic modulatory treatments is detailed.

Synthesis of Water-Solved Epoxy-Amine Oligomers and Receiving Coating Based on Them by Cathodic Electrodeposition

Kinetics of synthesis of epoxy-amine oligomers that are used as polielectrolytes in technology of cathodic electrodeposition was researched. Solubilityof the reaction product was researched by a correlation between pH and amount of neutralizing agent, molar mass distribution was researched for confirmation of expected molar mass. Pigment paste in three different options was made and used in a mixture with binder emulsion to receive coatings deposited on a cathode. Their properties were researched

Evaluation of an Environmental Transport Medium for Legionella pneumophila Recovery

The collection and storage of water-related matrices such as biofilm from collection to processing are critical for the detection of Legionella pneumophila by cultural and molecular tests. SRK™ is a liquid medium that acts both as an antimicrobial neutralizing agent and a transport medium for bacterial culture enumeration and is useful to maintain the stability of the sample from collection to analysis. The aims of this study were to evaluate Legionella pneumophila viability and bacterial nucleic acids’ stability in SRK™ medium over time at different storage conditions. Artificial bacterial inoculates with an approximate concentration of 104, 103 and 102 CFU/mL were made using Legionella pneumophila certified reference material suspended in SRK™ medium. Bacteria recovery was analyzed by cultural and molecular methods at time 0, 24 and 48 h at room temperature and at 0, 24, 48 and 72 h at 2–8 °C, respectively. SRK™ medium supported Legionella pneumophila culture viability with CFU counts within the expected range. The recovery after 72 h at 2–8 °C was 83–100% and 75–95% after 48 h at room temperature. Real-time PCR appropriately detected Legionella pneumophila DNA at each temperature condition, dilution and time point. Results demonstrated a good performance of SRK™ medium for the reliable recovery of environmental Legionella.

Agricultural and non-agricultural directions of bio-based sewage sludge valorization by chemical conditioning

This literature review outlines the most important—agricultural and non-agricultural—types of sewage sludge management. The potential of waste sludge protein hydrolysates obtained by chemical sludge conditioning was reported. The discussed areas include acidic and alkaline hydrolysis, lime conditioning, polyelectrolyte dewatering and other supporting techniques such as ultrasounds, microwave or thermal methods. The legislative aspects related to the indication of the development method and admission to various applications based on specified criteria were discussed. Particular attention was devoted to the legally regulated content of toxic elements: cadmium, lead, nickel, mercury, chromium and microelements that may be toxic: copper and zinc. Various methods of extracting valuable proteins from sewage sludge have been proposed: chemical, physical and enzymatic. While developing the process concept, you need to consider extraction efficiency (time, temperature, humidity, pH), drainage efficiency of post-extraction residues and directions of their management. The final process optimization is crucial. Despite the development of assumptions for various technologies, excess sewage sludge remains a big problem for sewage treatment plants. The high costs of enzymatic hydrolysis, thermal hydrolysis and ultrasonic methods and the need for a neutralizing agent in acid solubilization limit the rapid implementation of these processes in industrial practice. Graphical abstract

Identification of chlorophyll a-b binding protein AB96 as a novel TGFβ1 neutralizing agent

The discovery of compounds and proteins from plants has greatly contributed to modern medicine. Vernonia amygdalina Del. (Compositae) is used by humans and primates for a variety of conditions including parasitic infection. This paper describes the serendipitous discovery that V. amygdalina extract was able to bind to, and functionally inhibit, active TGFβ1. The binding agent was isolated and identified as chlorophyll a-b binding protein AB96. Given that active TGFβ1 contributes to the pathology of many infectious diseases, inhibiting these processes may explain some of the benefits associated with the ingestion of this species. This is the first plant-derived cytokine-neutralizing protein to be described and paves the way for further such discoveries.

Study of the process of neutralization of bromine – containing phthalate-type systems

The features of the final stages of the process of obtaining bromine-containing phthalate-type systems are considered. The factors that influence the quality of the target product were identified and the experiment plan was developed and implemented. As factors affecting the process, the following are selected: a – temperature, K; B – duration of neutralization, h; C – mass fraction of the neutralizing agent, %; D-the ratio of the used mass of the neutralizing agent to its calculated mass by the acid number of the reaction mass. The curves describing the dependence of the response function on various factors are processed using a graphical editor. The obtained curves are analyzed, indicating the presence of extremes and inflection points corresponding to the minimum acid number. Comparison of calculated and experimental data showed that the error of the result obtained by the regression equation is no more than 10%. Using balance calculations, it is established that the implementation of the process of neutralization of bromine-containing phthalate-type systems without a solvent will reduce losses by 3–5 times. Optimal conditions were found to ensure a low acid number of the target product after neutralization: the neutralization temperature is 315 K, the duration of neutralization is 0.5 h, the concentration of potassium hydroxide in an aqueous solution is 11 wt.%, the excess of the neutralizing agent per mol of the calculated 2.44 mol/mol. It is proved that conducting the process without solvents has a positive impact on both technological and economic indicators.the ralizing agent per mol is estimated at 2.44 mol/mol.

Additive-Manufactured Gyroid Scaffolds of Magnesium Oxide, Phosphate Glass Fiber and Polylactic Acid Composite for Bone Tissue Engineering

Composites of biodegradable phosphate glass fiber and polylactic acid (PGF/PLA) show potential for bone tissue engineering scaffolds, due to their ability to release Ca, P, and Mg during degradation, thus promoting the bone repair. Nevertheless, glass degradation tends to acidify the surrounding aqueous environment, which may adversely affect the viability and bone-forming activities of osteoblasts. In this work, MgO was investigated as a neutralizing agent. Porous network-phase gyroid scaffolds were additive-manufactured using four different materials: PLA, MgO/PLA, PGF/PLA, and (MgO + PGF)/PLA. The addition of PGF enhanced compressive properties of scaffolds, and the resultant scaffolds were comparably strong and stiff with human trabecular bone. While the degradation of PGF/PLA composite induced considerable acidity in degradation media and intensified the degradation of PGF in return, the degradation media of (MgO + PGF)/PLA maintained a neutral pH close to a physiological environment. The experiment results indicated the possible mechanism of MgO as the neutralizing agent: the local acidity was buffered as the MgO reacted with the acidic degradation products thereby inhibiting the degradation of PGF from being intensified in an acidic environment. The (MgO + PGF)/PLA composite scaffold appears to be a candidate for bone tissue engineering.