A review of the effects of metallic nanoparticles on fish

Many important discoveries have been made in the field of nanotechnology in the last 40 years. Since then, nanoparticles became nearly ubiquitous. With their spreading use, safety concerns have warranted extensive research of nanotoxicity. This paper offers information about the occurrence, transport, and behaviour of metallic nanoparticles in the aquatic environment. It further summarizes details about parameters that dictate the toxicity of nanoparticles and discusses the general/common mechanisms of their toxicity. This review also focuses on fish exposure to nanoparticles, including the possibility of trophic transport through the food chain. Information on some of the most frequently used metallic nanoparticles, such as silver, gold, and titanium dioxide, is further elaborated on.

Effect of chloretracycline and albendazole on alkaline phosphatase activity of carp gill (Cyprinus carpio L.)

The effect of the antibiotic chlortetracycline and the anthelmintic albendazole on morphological features, internal organs, alkaline phosphatase activity and protein content in the gills of two-year-old carp was studied. It was found that chlortetracycline and albendazole at the studied concentrations in water and short-term action do not affect the morphological features, behavior and internal organs, but change the alkaline phosphatase activity of the gills of two-year-old carp. The antibiotic chlortetracycline at concentrations of 1.10, 3.15, and 6.30 mg/dm3 of water and fish exposure for 72 h reduced the alkaline phosphatase activity of gills in carp of the first experimental group by 2.5 times, in the second – by 2.8 times and in the third – by 2.7 times regard the control; it did not affect the protein content in the gills, morphological features and internal organs of fish. We registered an increase in alkaline phosphatase activity of gills in two-year-old carp under effect of anthelmintic albendazole at at the concentration of 1.0 mg/dm3 by 28.2%, while albendazole concentration of 0.2 and 0.5 mg/dm3 did not affect the alkaline phosphatase activity as well as the level of protein in fish body. Our results indicate an important role of gill alkaline phosphatase in assessing the negative effects of antibiotics and water anthelmintics in freshwater fish.

Late onset periprosthetic infection of the hip caused by the fish pathogen Lactococcus garvieae in a patient not associated with fish exposure

Lactococcus garvieae is a fish pathogen, rarely causing opportunistic infections in humans. There are only a few cases reported in the literature, mainly endocarditis, suggesting an association with raw fish consumption. We report a case of a periprosthetic hip infection successfully treated with a two-stage revision surgery.

Toxicity of Cyanopeptides from Two Microcystis Strains on Larval Development of Astyanax altiparanae

Absorption and accumulation of bioavailable cyanobacterial metabolites (including cyanotoxins) are likely in fish after senescence and the rupturing of cells during bloom episodes. We determined the toxicity of cyanopeptides identified from two strains of Microcystis (M. panniformis MIRS-04 and M. aeruginosa NPDC-01) in a freshwater tropical fish, Astyanax altiparanae (yellowtail tetra, lambari). Aqueous extracts of both Microcystis strains were prepared in order to simulate realistic fish exposure to these substances in a freshwater environment. Both strains were selected because previous assays evidenced the presence of microcystins (MCs) in MIRS-04 and lack of cyanotoxins in NPDC-01. Identification of cyanobacterial secondary metabolites was performed by LC-HR-QTOF-MS and quantification of the MC-LR was carried out by LC-QqQ-MS/MS. MIRS-04 produces the MCs MC-LR, MC-LY and MC-HilR as well as micropeptins B, 973, 959 and k139. NPCD-01 biosynthetizes microginins FR1, FR2/FR4 and SD-755, but does not produce MCs. Larval fish survival and changes in morphology were assessed for 96 h exposure to aqueous extracts of both strains at environmentally relevant concentrations from 0.1 to 0.5 mg (dry weight)/mL, corresponding to 0.15 to 0.74 μg/mL of MC-LR (considering dried amounts of MIRS-04 for comparison). Fish mortality increased with concentration and time of exposure for both strains of Microcystis. The frequencies of morphological abnormalities increased with concentration in both strains, and included abdominal and pericardial oedema, and spinal curvature. Results demonstrate that toxicity was not solely caused by MCs, other classes of cyanobacterial secondary metabolites contributed to the observed toxicity.

Future ocean warming may prove beneficial for the northern population of European seabass, but ocean acidification does not

The world's oceans are acidifying and warming due to increasing amounts of atmospheric CO2. Thermal tolerance of fish much depends on the cardiovascular ability to supply the tissues with oxygen. The heart itself is highly dependent on oxygen and heart mitochondria thus might play a key role in shaping an organism's tolerance to temperature. The present study aimed to investigate the effects of acute and chronic warming on respiratory capacities of European sea bass (Dicentrarchus labrax L.) heart mitochondria. We hypothesized that acute warming would impair mitochondrial respiratory capacities, but be compensated after long-term. Increasing PCO2 may cause intracellular changes, likely further constricting cellular energy metabolism. We found increased leak respiration rates in acutely warmed heart mitochondria of cold-conditioned fish in comparison to measurements at their rearing temperature, suggesting a lower aerobic capacity to synthesize ATP. However, thermal acclimation led to increased mitochondrial functionality, e.g. higher RCRo in heart mitochondria of warm-conditioned compared to cold-conditioned fish. Exposure to high PCO2 synergistically amplified the effects of acute and long-term warming, but did not result in changes by itself. We explained the high ability to maintain mitochondrial function under OA with the fact that seabass are moving between various environmental conditions. Improved mitochondrial capacities after warm conditioning could be due to the origin of this species in the warm waters of the Mediterranean. Our results also indicate that seabass are not yet fully adapted to the colder temperatures in their northern distribution range and might benefit from warmer temperatures.

Mercury, selenium and fish oils in marine food webs and implications for human health

Humans who eat fish are exposed to mixtures of healthful nutrients and harmful contaminants that are influenced by environmental and ecological factors. Marine fisheries are composed of a multitude of species with varying life histories, and harvested in oceans, coastal waters and estuaries where environmental and ecological conditions determine fish exposure to both nutrients and contaminants. Many of these nutrients and contaminants are thought to influence similar health outcomes (i.e., neurological, cardiovascular, immunological systems). Therefore, our understanding of the risks and benefits of consuming seafood require balanced assessments of contaminants and nutrients found in fish and shellfish. In this paper, we review some of the reported benefits of fish consumption with a focus on the potential hazards of mercury exposure, and compare the environmental variability of fish oils, selenium and mercury in fish. A major scientific gap identified is that fish tissue concentrations are rarely measured for both contaminants and nutrients across a range of species and geographic regions. Interpreting the implications of seafood for human health will require a better understanding of these multiple exposures, particularly as environmental conditions in the oceans change.