Czech Farmers' Experience with Bt Maize: Fulfilment, and the Opposite, of Monsanto's Promises

Genetically modified (GM) Bt maize MON810 is promoted as being beneficial for farmers and the environment. The aim of this study was to compare particular claims of the benefits associated with this GM maize with Czech farmers' practice. Ten semi-structured interviews and 27 questionnaires obtained from Czech farmers who had grown Bt maize for at least one year in 2005–2016 were analysed qualitatively. The alleged benefits of MON810 cultivation regarding lower levels of fungal disease infestation, yield increase, reduction of insecticide usage, lowered costs, time saving and simple manipulation were enjoyed by a varying proportion of Czech GM farmers. In contrast, some farmers experienced the opposite of the claimed benefit regarding yield, costs, manipulation and time requirements. The exception was the 100% control of the European Corn Borer (ECB). The current and previous studies suggest that most of the benefits manifest themselves rather on farms under high ECB pressure. Seen from an economic viewpoint, the cultivation of Bt maize could be recommended in areas with persistent high corn borer pressure. However, the benefit to the environment is challenged and it is suggested that farmers should employ a complex of cultural control methods.

Pathways of Conflict: Lessons from the Cultivation of MON810 in Germany in 2005–2008 for Emerging Conflicts over New Breeding Techniques

The paper uses qualitative interviews and document analysis to examine conflicts over plant and animal breeding techniques from the perspectives of Social and Political Ecology. It asks how past conflicts over genetically modified organisms (GMOs) can inform understandings of possible trajectories of emerging conflicts over new breeding techniques (NBTs) such as CRISPR/Cas genome editing. Case studies of conflicts in three areas where the transgenic maize MON810 was cultivated in Germany from 2005–2008 show that the escalation of conflict coincided with the first tangible presence of these already controversial organisms in the rural landscape. Location-specific interlinkages between discursive and material dimensions gave rise to different pathways of conflict in the three areas studied. These empirical results inform the analysis of emerging conflicts over NBTs in Germany and the United Kingdom. The future of NBTs in both countries is still open, and the divergence of regulatory frameworks in Europe could lead to the development of ‘NBT hotspots’ located in particular European countries, provoking an escalation of conflict in areas where commercial application takes place. The paper concludes by examining the potential for a politicization of future conflicts to encompass wider issues related to the transformation of agricultural systems towards sustainability.

Expression profiling of key pathways in rat liver after a one-year feeding trial with transgenic maize MON810

In a recent one-year feeding study, we observed no adverse effects on tissue level in organs of rats fed with the genetically-modified maize MON810. Here, we assessed RNA expression levels of 86 key genes of the apoptosis-, NF-кB-, DNA-damage response (DDR)-, and unfolded-protein response (UPR) pathways by RT-qPCR in the rat liver. Male and female rats were fed either with 33% MON810 (GMO), isogenic- (ISO), or conventional maize (CONV) and RNAs were quantified from eight rats from each of the six feeding groups. Only Birc2 transcript showed a significant (p ≤ 0.05) consistent difference of ≥1.5-fold between the GMO and ISO groups in both sexes. Unsupervised cluster analysis showed a strong separation of male and female rats, but no clustering of the feeding groups. Individual analysis of the pathways did not show any clustering of the male or female feeding groups either, though transcript levels of UPR pathway-associated genes caused some clustering of the male GMO and CONV feeding group samples. These differences were not seen between the GMO and ISO control or within the female cohort. Our data therefore does not support an adverse effect on rat liver RNA expression through the long-term feeding of MON810 compared to isogenic control maize.

Annual replication is essential in evaluating the response of the soil microbiome to the genetic modification of maize in different biogeographical regions

The importance of geographic location and annual variation on the detection of differences in the rhizomicrobiome caused by the genetic modification of maize (Bt-maize, event MON810) was evaluated at experimental field sites across Europe including Sweden, Denmark, Slovakia and Spain. DNA of the rhizomicrobiome was collected at the maize flowering stage in three consecutive years and analyzed for the abundance and diversity of PCR-amplified structural genes of Bacteria, Archaea and Fungi, and functional genes for bacterial nitrite reductases (nirS, nirK). The nirK genes were always more abundant than nirS. Maize MON810 did not significantly alter the abundance of any microbial genetic marker, except for sporadically detected differences at individual sites and years. In contrast, annual variation between sites was often significant and variable depending on the targeted markers. Distinct, site-specific microbial communities were detected but the sites in Denmark and Sweden were similar to each other. A significant effect of the genetic modification of the plant on the community structure in the rhizosphere was detected among the nirK denitrifiers at the Slovakian site in only one year. However, most nirK sequences with opposite response were from the same or related source organisms suggesting that the transient differences in community structure did not translate to the functional level. Our results show a lack of effect of the genetic modification of maize on the rhizosphere microbiome that would be stable and consistent over multiple years. This demonstrates the importance of considering annual variability in assessing environmental effects of genetically modified crops.

Diversity of insects under the effect of Bt maize and insecticides

ABSTRACT: The genetically modified maize to control some caterpillars has been widely used in Brazil. The effect of Bt maize and insecticides was evaluated on the diversity of insects (species richness and abundance), based on the insect community, functional groups and species. This study was conducted in genetically modified maize MON810, which expresses the Cry1Ab protein from Bacillus thuringiensis Berliner, and conventional maize with and without insecticide sprays (lufenuron and lambda-cyhalothrin) under field conditions in Ponta Grossa (Paraná state, Brazil). Insect samplings were performed by using pitfall trap, water tray trap and yellow sticky card. A total of 253,454 insects were collected, distributed among nine orders, 82 families and 241 species. No differences were observed in the insect community based on the richness, diversity and evenness indices. Predators and pollinators were more abundant in genetically modified maize. Parasitoids, detritivores, sap-sucking herbivores and chewing herbivores were more abundant in conventional maize with insecticide sprays. Significant differences were found for the species Colopterus sp., Colaspis occidentalis (L.) and Nusalala tessellata (Gerstaecker) which were most abundant in Bt maize, and Dalbulus maidis and Condylostylus sp.2 in conventional maize.