How Does a Cell Change Flow Direction Due to a Micro Groove?

The change in direction of a cell flowing over an oblique micro groove has been analyzed in vitro. The micro flow-channel (0.05 mm height x 1 mm width x 25 mm length) with oblique micro grooves (4.5 μm depth) was manufactured on a polydimethylsiloxane (PDMS) disk by the micromachining technique. The angle between the main flow direction and the longitudinal axis of the groove is 45 degrees. The effect of variation of the groove width (0.03 mm, 0.04 mm, and 0.05 mm) was studied. Myoblasts (C2C12: mouse myoblast cell line) were used in the test. The main flow velocity (0.02 mm/s < vx < 0.23 mm/s) of the medium was controlled by the pressure difference between the inlet and the outlet. The shape of each flowing cell was tracked on a movie recorded by the camera attached to the eyepiece of the microscope. The experimental results show that the change of the direction of each cell by each groove depends on the shape of the cell, which depends on both the shape of the cell and the width of the groove.

Movement of Cell Flowing Over Oblique Micro Grooves in Flow Channel

A micro flow-channel with bottom-microgrooves has been manufactured by photolithography technique for cell sorting. The movement of each cell passing over microgrooves has been analyzed in relation to cell deformation and alignment in vitro. The flow path (height 0.05 mm × width 1 mm × length 25 mm) between the two transparent PDMS disks has rectangular microgrooves (4.5 μm deep, 0.2 mm long) on the bottom. Variations are made in groove widths (0.03 mm, 0.04 mm, and 0.05 mm). The angle between the flow direction and the longitudinal axis of the groove is 45 degrees. Myoblasts (C2C12: mouse myoblast line) were used in the flow test. The main flow velocity of the medium (0.02 mm/s &lt; vx &lt; 0.23 mm/s) was controlled by the pressure difference between the inlet and the outlet. The shape of each flowing cell was tracked in a movie recorded by a camera attached to the eyepiece of the microscope. Experimental results show that the movement perpendicular to the main flow direction in the micro-groove can distinguish cells in relation to smaller deformations and larger alignment changes.

Movement of Myoblast Flowing Through Electric Field Perpendicular to Flow Channel

The sorting technology with little invasion to cells would be applied to regenerative medicine and diagnosis. In this study, dielectrophoresis is focused on. The dielectrophoretic effect on the flowing myoblasts was maximized by adjusting several parameters: the shape of the electrodes, the amplitude and frequency of the alternating current. The suspension of C2C12 (mouse myoblast cell line) was injected into the channel, and the movement of each flowing cell was analyzed at the microscopic movie image. A pair of titanium-coated (200 nm thick) asymmetric surface electrodes (a triangular electrode with a tip angle of 0.35 rad and a rectangular reference electrode with a flat edge) was manufactured by photolithography technique. With the alternating square cyclic wave at the frequency of 3 MHz and the amplitude of current of ± 7.5 mA, 70 μm movement along the electric field (perpendicular to the main flow direction) of the cell was obtained. The movement along the electric field is governed by several parameters of the cell: the diameter, the deformation ratio, and the direction of the major axis. The method can be applied to cell sorting.

Large Eddy Simulation of the 7-7-7 Shaped Film Cooling Hole at Axial and Compound Angle Orientations

Shaped film cooling holes are used extensively for film cooling in gas turbines due to their superior performance in keeping coolant attached to the surface, relative to cylindrical holes. However, fewer studies have examined the impact of the orientation of the shaped hole axis relative to the main flow direction, known as a compound angle. A compound angle can occur intentionally due to manufacturing, or unintentionally due to changes in the main flow direction at off-design conditions. In either case, the compound angle causes the film cooling jet to roll up into a strong streamwise vortex that changes the lateral distribution of coolant, relative to the pair of vortices that develop from an axially oriented film cooling hole. In this study, Large Eddy Simulation (LES) using the Wall-Adapting Local Eddy Viscosity (WALE) model was performed on the publicly available 7-7-7 shaped film cooling hole, at two orientations (0°, 30°) and two blowing ratios (M = 1, 3). Laterally-averaged film effectiveness was largely unchanged by a compound angle at a blowing ratio of 1, but improved at a blowing ratio of 3. For both blowing ratios, the lateral distribution of film was more uniform with the addition of a 30° compound angle. Both wall normal and lateral turbulent convective heat transfer was increased by the addition of a compound angle at both blowing ratios.

Heat Transfer Characterization Inside a Rotating Rib Roughened Cooling Channel With Multiple Heated Walls

This work experimentally investigates the effects of walls heating conditions on the heat transfer coefficient distribution inside a rotating cooling channel. The model has a square cross-section with a hydraulic diameter of 50 mm and one ribbed side. The ribs are perpendicular to the main flow direction, the rib pitch-to-height ratio is 10, and the blockage ratio is 10%. Detailed heat transfer measurements were performed employing the liquid crystals thermography in steady-state approach under two thermal boundary conditions: only ribbed wall heated or equal heat fluxes set to ribbed wall and sidewalls. Inlet Reynolds number was 20000 and the tests were conducted in both static and rotating conditions up to a rotation number of 0.18, with the ribbed wall acting as trailing edge side. The results show that the wall heating conditions have a negligible effect on the heat transfer distribution for the stationary case, whereas, they lead to a modification of the heat transfer distribution under rotation with an overall enhancement when three walls are heated.

Research on the silver in the world flowing into Qing Dynasty

In the 80 years of foreign trade in the Qing Dynasty, the main flow direction of silver was from Spain and Britain to Qing Dynasty, which is one of the main contents; the other is the materials, process and variety design of the silver flowing into the Qing Dynasty.

Three-Dimensional Couette Flow of a Second-Grade Fluid Along Periodic Injection/Suction

This work explores the three-dimensional laminar flow of an incompressible second-grade fluid between two parallel infinite plates. The assumed suction velocity comprises a basic steady dispersal with a superimposed weak transversally fluctuating distribution. Because of variation of suction velocity in transverse direction on the wall, the problem turns out to be three-dimensional. Analytic solutions for velocity field, pressure and skin friction are presented and effects of dimensionless parameters emerging in the model are discussed. It is observed that the non-Newtonian parameter plays dynamic part to rheostat the velocity component along main flow direction.

Geomorphological structure and neotectonics of the Lena delta

New data about geomorphological structure and neotectonic movements of the Lena Delta is presented. Thet are based on results of the Russian-German expeditions Lena-2013, Lena-2014, Lena-2015 and Lena-2018 and include geomorphological profiling with high quality satellite instruments and a number of radiocarbon dates on the Sobo-Sise, Kurungnakh, Jangylakh-Sis and Khardang-Sise islands. These islands consist of the Late Pleistocene Ice Complex (IC) remnants eroded by river and sea, and the first terrace of the Delta, which adjoins the remnants. The first terrace started to form 8,000 years ago in the western part of the Delta and was finally formed in the last millennium in the eastern part of the Delta. From the previous works, it is known that the western part of the Delta is higher than the eastern part. In our work, we explain it by the eneven movements of the Earth’s crust in this region. The aim of the paper is to study the quantitative characteristics of the tectonic movements in the Lena River Delta. For this purpose we present the geomorphological schemes and descriptions of the islands mentioned and compare the terraces heights in the different parts of the Delta. In the Late Pleistocene, according to the heights of the IC remnants, the western part of the Delta rose 1 mm per year faster than its eastern part. In the Holocene the speed difference increased to approximately 2 mm per year, which is shown by the terrace surface’s altitude. The amplitude between the western and eastern parts of this surface is about 4 m. Finally, according to 60-years observation period of the water level in the Laptev Sea, the modern speed difference of the western and eastern parts movement in the Lena Delta is 2 mm per year. As a result, we can observe changes in the main flow direction in the Delta channels from the Olenekskaya branch at the beginning of Holocene to the Bykovskaya branch today.

A Numerical Analysis of Pressure Pulsation Characteristics Induced by Unsteady Blood Flow in a Bileaflet Mechanical Heart Valve

The leaflet vibration phenomenon in bileaflet mechanical heart valves (BMHVs) can cause complications such as hemolysis, leaflet damage, and valve fracture. One of the main reasons for leaflet vibration is the unsteady blood flow pressure pulsation induced by turbulent flow instabilities. In this study, we performed numerical simulations of unsteady flow through a BMHV and observed pressure pulsation characteristics under different flow rates and leaflet fully opening angle conditions. The pressure pulsation coefficient and the low-Reynolds k-ω model in CFD (Computational Fluid Dynamics) software were employed to solve these problems. Results showed that the level of pressure pulsation was highly influenced by velocity distribution, and that the higher coefficient of pressure pulsation was associated with the lower flow velocity along the main flow direction. The influence of pressure pulsation near the trailing edges was much larger than the data obtained near the leading edges of the leaflets. In addition, considering the level of pressure pulsation and the flow uniformity, the recommended setting of leaflet fully opening angle was about 80°.

Conditions For Physical Modelling of The Spread of Contaminants in Groundwater

The paper contains a review of dimensionless experimental dependencies determining the coefficient of transverse dispersion Dy. In the course of the dimensional analysis, dimensionless experimental dependencies describing the dispersion of pollutants in groundwater with hydraulic and physical parameters of soil in the case of steady and uniform groundwater motion were presented. The analysis allowed the determination of dimensionless practical relationships defining the dispersion factor in the perpendicular direction to the main flow direction.