A High Separation Factor for 165Er from Ho for Targeted Radionuclide Therapy

Background: Radionuclides emitting Auger electrons (AEs) with low (0.02–50 keV) energy, short (0.0007–40 µm) range, and high (1–10 keV/µm) linear energy transfer may have an important role in the targeted radionuclide therapy of metastatic and disseminated disease. Erbium-165 is a pure AE-emitting radionuclide that is chemically matched to clinical therapeutic radionuclide 177Lu, making it a useful tool for fundamental studies on the biological effects of AEs. This work develops new biomedical cyclotron irradiation and radiochemical isolation methods to produce 165Er suitable for targeted radionuclide therapeutic studies and characterizes a new such agent targeting prostate-specific membrane antigen. Methods: Biomedical cyclotrons proton-irradiated spot-welded Ho(m) targets to produce 165Er, which was isolated via cation exchange chromatography (AG 50W-X8, 200–400 mesh, 20 mL) using alpha-hydroxyisobutyrate (70 mM, pH 4.7) followed by LN2 (20–50 µm, 1.3 mL) and bDGA (50–100 µm, 0.2 mL) extraction chromatography. The purified 165Er was radiolabeled with standard radiometal chelators and used to produce and characterize a new AE-emitting radiopharmaceutical, [165Er]PSMA-617. Results: Irradiation of 80–180 mg natHo targets with 40 µA of 11–12.5 MeV protons produced 165Er at 20–30 MBq·µA−1·h−1. The 4.9 ± 0.7 h radiochemical isolation yielded 165Er in 0.01 M HCl (400 µL) with decay-corrected (DC) yield of 64 ± 2% and a Ho/165Er separation factor of (2.8 ± 1.1) · 105. Radiolabeling experiments synthesized [165Er]PSMA-617 at DC molar activities of 37–130 GBq·µmol−1. Conclusions: A 2 h biomedical cyclotron irradiation and 5 h radiochemical separation produced GBq-scale 165Er suitable for producing radiopharmaceuticals at molar activities satisfactory for investigations of targeted radionuclide therapeutics. This will enable fundamental radiation biology experiments of pure AE-emitting therapeutic radiopharmaceuticals such as [165Er]PSMA-617, which will be used to understand the impact of AEs in PSMA-targeted radionuclide therapy of prostate cancer.

Enhanced XUV harmonics generation from diatomic gases using two orthogonally polarized laser fields

Enhanced high repetition rate coherent extreme ultraviolet (XUV) harmonics represent efficient probe of electron dynamics in atoms, molecules and solids. In this work, we used orthogonally-polarized two-color laser field to generate strong even and odd high order harmonics from molecular gas targets. The dynamics of odd and even harmonics from O2, and N2 gases were investigated by employing single- and two-color laser fields using the fundamental radiation and second harmonic of 1030 nm, 37 fs, 50 kHz pulses. The relative efficiencies of harmonics were analyzed as a function of the thickness of the barium borate crystal used for second harmonic generation. Defocusing-assisted phase matching conditions were achieved in N2 gas for different groups of XUV harmonics.

Об излучении гармоник в рентгеновских лазерах на свободных электронах с изменяемым параметром дипольности ондуляторов

X-ray free electron lasers (FELs) produce ultra short bursts of coherent radiation at the wavelengths ~1–100 . In the absence of ready seed in this range, the fundamental tone is usually radiated in self-amplified spontaneous emission (SASE) FELs. With the goal to achieve maximum performance in minimum size and cost of a FEL, we study the harmonic multiplication in resonating undulator cascades as viable alternative to the SASE fundamental radiation. The harmonic power evolution along FELs is analyzed for the SASE and the harmonic self-seed. The analytical results are compared with available data for soft X-rays FLASH2. The possibility of harmonic radiation is studied for FELs with variable deflection parameter k: FLASH2, SwissFEL and currently built LCLS-II. We demonstrate earlier growth of the harmonic power in a buncher as compared with the SASE regime at the same wavelength. The difference is explained theoretically by the harmonic bunching growth in the buncher, as compared with that in the SASE FEL at the same wavelength. Excellent characteristics of the SwissFEL allow amplification of its third harmonic. We propose the harmonic self-seed, which would allow 10 m shorter undulator length for saturation at the same power, and up to 30% shorter radiation wavelength with the existing electron beam. At the LCLS-II, good bunching at the third harmonic wavelength in a dedicated buncher allows early and strong third harmonic growth. However, further resonating amplifier requires small value of k in LCLS-II undulators, which makes the amplification slow. We propose using the full amplification of the third FEL harmonic in LCLS-II undulators with bunching disruption between them at the wavelength of the fundamental. We show that this scheme allows >1 GW FEL power at λ=0.25 nm wavelength achievable already at ~40 m of the undulators with the electron beam energy E=4 GeV.

LONG-TERM GENETIC AND EPIGENETIC DISORDERS IN PERSONS EXPOSED TO IONIZING RADIATION AND THEIR DESCENDANTS (review)

The review is devoted to long-term genetic and epigenetic disorders in exposed individuals and their descendants, namely to cytogenetic effects in the Chornobyl NPP accident clean-up workers and their children, DNA methylation as an epigenetic modification of human genome. Data presented in review expand the understanding of risk of the prolonged exposure for the present and future generations, which is one of key problems posed by fundamental radiation genetics and human radiobiology. The Scientific Council meeting of NAMS approved the NRCRM Annual Report. Key words: Chornobyl NPP accident, cytogenetic effects, DNA methylation.

Spectroscopic Investigation of Laser-Induced Graphene Plasma

In this paper, graphene plasma was obtained through the interaction of the fundamental radiation from a pulsed Nd:YAG laser at the fundamental wavelength of 1064 nm focused onto a solid plane of graphene material. This reaction was carried out under conditions of an atmospheric status. The resulting plasma was tested using an optical emission spectroscopy technique. The temperature of the electrons is calculated by the tow line ratio of C I and C II emission lines singly ionised, and the density of the plasma electron is calculated with Saha-Boltzmann equation. The upper limit of the electron temperature was approximately 1.544 eV. The corresponding electron density was 11.5×1015 cm-3. Then the electron temperature decreased when the energy was 300 mJ and it was near 1.462 eV, corresponding to the density of those electrons 8.7×1015 cm-3.

“Shutdown” of the Proton Exchange Channel Waveguide in the Phase Modulator under the Influence of the Pyroelectric Effect

It is shown that the termination of the channeling of the fundamental radiation mode in the waveguide can be observed upon heating of an optical integrated circuit based on proton exchange channel waveguides formed in a lithium niobate single crystal. This process is reversible, but restoration of waveguide performance takes tens of minutes. The effect of the waveguide disappearance is observed upon rapid heating (5 K/min) from a low temperature (minus 40 °C). This effect can lead to a temporary failure of navigation systems using fiber optic gyroscopes with modulators based on a lithium niobate crystal.

Light source based on a 100 mm-long monolithic undulator magnet with a very short 4 mm-period length

A novel method to fabricate undulator magnets of a-few-millimetre-period length is being explored. Plate-type magnets, 100 mm-long with 4 mm-period length, have been successfully fabricated. They produce an undulator field of approximately 3 kG at a gap of 1.6 mm. Prototype undulators based on this technology have been constructed. Field measurements and characterization show that the quality of the undulator field of these plate magnets is sufficient for an undulator light source, and the calculated spectrum shows that the fundamental radiation emitted from this field is quite satisfactory. Test experiments for light generation using a real electron beam have been carried out at a test accelerator at the Research Center for Electron Photon Science (ELPH), Tohoku University, Japan, which is able to realize optics conditions to accept a very short gap of ∼1.6 mm. First observation and characterization of blue light was successfully accomplished.

"Shutdown" of the Proton Exchange Channel Waveguide in the Phase Modulator under the Influence of the Pyroelectric Effect

It is shown that the termination of the channeling of the fundamental radiation mode in the waveguide can be observed upon heating of an optical integrated circuit based on proton exchange channel waveguides formed in a lithium niobate single crystal. This process is reversible, but restoration of waveguide performance takes tens of minutes. The effect of the waveguide disappearance is observed upon rapid heating (5 °C/min) from a low temperature (minus 40 °C). This effect can lead to a temporary failure of navigation systems using fiber optic gyroscopes with modulators based on a lithium niobate crystal