Histological assessment of the efficiency of rabbit serum in healing skin wounds

Aim: This study aimed to investigate the impact of rabbit serum on skin wound healing with the help of histological examination. Materials and Methods: A total of ten indigenous rabbits were used in this study. The animals were divided into two groups: control and serum- treated. The histological assessment was done with a paraffin embedding technique and the histological sections were stained with H&E stain. Results: Severe infiltration of polymorphonuclear leukocytes with severe fibrin deposits were seen in serum treated group at 2 days post-injury; at 7 days post-injury the changes revealed moderate fibroplasia, fibrin deposit and severe infiltration of both mononuclear and polymorphonuclear leukocytes; at 14 days post-injury, there were marked epithelization and dermal deposition of collagen fibers; and at 21 days post-injury, the epidermis completed epithelization and the dermis showed neither fibroplasia nor infiltration of mononuclear and polymorphonuclear leukocytes. Conclusion: The results indicated that rabbit's serum can prevent wound infection, accelerate epithelialization and cutaneous regeneration with less granulation.

Development of a porcine model for assessment of mucosal repair following endoscopic resection of the lower gastrointestinal tract

Background and aims Endoscopic mucosal resection (EMR) is widely performed for the treatment of colorectal polyps. However, the pathophysiological mechanisms of mucosal repair, including in situations at high risk of post-polypectomy bleeding, remain largely unknown. The objective of our study was to develop a porcine model of EMR in the lower gastrointestinal tract to monitor mucosal wound healing over time. Methods Under general anesthesia, five large wounds were created in the lower gastrointestinal tract at different times, i. e. at day 0, 3, 7, 10, and 14, by multiband EMR, in each of the six pigs in the study. A colorectal resection was performed at day 14 and the animal euthanized. Repeated endoscopic and endomicroscopic examination, and histological analysis were performed. Results No complications occurred and all animals reached the study end point. The endoscopic aspect of wound healing evolved into different phases with first a fibrin deposit covering the wounds which then gave way to granulomatous tissue. The size of the wound regressed significantly as early as day 3. Re-epithelialization of the wound started from day 7, and neo-mucosal crypts appeared from day 10. The endomicroscopic analysis described a ‘ground glass appearance’ from day 3 and irregular crypts from day 10, which was consistent with histological data. Good agreement between macroscopic, endomicroscopic, and histological parameters of mucosal wound healing was observed in vivo. Conclusion This study demonstrates for the first time the feasibility of an experimental in vivo porcine model of lower gastrointestinal endoscopic resections to monitor tissue repair. This model might be helpful to document pharmacological approaches for preventing complications of endoscopic procedures performed in humans.

Tissue Characterization with Gray-level Histogram Width in Obstetrics and Gynecology

ABSTRACT Aim Clinical ultrasound tissue characterization, using usual B-mode devices. Materials and methods Malignant neoplasia in ovary, uterine cervix, and endometrium; placental intervillous space fibrin deposit; fetal growth restriction; fetal brain, fetal lung immaturity; meconium-stained amniotic fluid and healthy adult liver; Tissue was characterized by gray-level histogram width (GLHW) divided by full gray scale length. Results Malignant GLHW was higher than in benign one (it was malignant if the GLHW was 50% or more in ovary, uterine cervix, and endometrium). The GLHW of placental fibrin deposit was higher than normal placenta. It was reduced by heparin and normal neonate was obtained. Fetal brain echo density, immature fetal lung, and meconium-stained amniotic fluid were diagnosed by GLHW, and normal adult liver GLHW was studied. Helsinki declaration was followed. Conclusion The GLHW tissue characterization objectively diagnosed ultrasound B-mode image in obstetrics and gynecology; thus, it would also be applied in common adult human cases. How to cite this article Maeda K, Kihaile PE, Ito T, Utsu M, Yamamoto N, Serizawa M. Tissue Characterization with Gray-level Histogram Width in Obstetrics and Gynecology. Donald School J Ultrasound Obstet Gynecol 2017;11(1):7-10.