Effects of excessive fibrin deposit and polylactide adhesion barrier on wound healing in thyroidectomy murine wound model

Head & Neck ◽  
2018 ◽  
Vol 40 (6) ◽  
pp. 1207-1213
Author(s):  
Yun-Sung Lim ◽  
Hee-Bok Kim ◽  
Joo Hyun Park ◽  
Chang Gun Cho ◽  
Seok-Won Park ◽  
...  
Keyword(s):  
Wound Healing ◽  
Adhesion Barrier ◽  
Wound Model ◽  
Fibrin Deposit

ultrastructural process of intestinal wound healing

1995 ◽  
Vol 53 ◽  
pp. 1024-1025
Author(s):  
Rick L. Vaughn ◽  
Shailendra K. Saxena ◽  
John G. Sharp
Keyword(s):  
Wound Healing ◽  
Epithelial Cells ◽  
Smooth Muscles ◽  
Microscopic Level ◽  
Light Microscopic Level ◽  
Ileal Mucosa ◽  
Wound Model ◽  
Serosal Surface ◽  
Complex Process ◽  
Orderly Fashion

We have developed an intestinal wound model that includes surgical construction of an ileo-cecal patch to study the complex process of intestinal wound healing. This allows approximation of ileal mucosa to the cecal serosa and facilitates regeneration of ileal mucosa onto the serosal surface of the cecum. The regeneration of ileal mucosa can then be evaluated at different times. The wound model also allows us to determine the rate of intestinal regeneration for a known size of intestinal wound and can be compared in different situations (e.g. with and without EGF and Peyer’s patches).At the light microscopic level it appeared that epithelial cells involved in regeneration of ileal mucosa originated from the enlarged crypts adjacent to the intestinal wound and migrated in an orderly fashion onto the serosal surface of the cecum. The migrating epithelial cells later formed crypts and villi by the process of invagination and evagination respectively. There were also signs of proliferation of smooth muscles underneath the migratory epithelial cells.

scholarly journals Injectable Self-Healing Adhesive pH-Responsive Hydrogels Accelerate Gastric Hemostasis and Wound Healing

2021 ◽  
Vol 13 (1) ◽  
Author(s):  
Jiahui He ◽  
Zixi Zhang ◽  
Yutong Yang ◽  
Fenggang Ren ◽  
Jipeng Li ◽  
...  
Keyword(s):  
Wound Healing ◽  
Endoscopic Treatment ◽  
Healing Process ◽  
Gelation Time ◽  
Type I ◽  
Self Healing ◽  
Ph Responsive ◽  
Gastric Hemorrhage ◽  
Wound Model ◽  
Arterial Bleeding

AbstractEndoscopic mucosal resection (EMR) and endoscopic submucosal dissection (ESD) are well-established therapeutics for gastrointestinal neoplasias, but complications after EMR/ESD, including bleeding and perforation, result in additional treatment morbidity and even threaten the lives of patients. Thus, designing biomaterials to treat gastric bleeding and wound healing after endoscopic treatment is highly desired and remains a challenge. Herein, a series of injectable pH-responsive self-healing adhesive hydrogels based on acryloyl-6-aminocaproic acid (AA) and AA-g-N-hydroxysuccinimide (AA-NHS) were developed, and their great potential as endoscopic sprayable bioadhesive materials to efficiently stop hemorrhage and promote the wound healing process was further demonstrated in a swine gastric hemorrhage/wound model. The hydrogels showed a suitable gelation time, an autonomous and efficient self-healing capacity, hemostatic properties, and good biocompatibility. With the introduction of AA-NHS as a micro-cross-linker, the hydrogels exhibited enhanced adhesive strength. A swine gastric hemorrhage in vivo model demonstrated that the hydrogels showed good hemostatic performance by stopping acute arterial bleeding and preventing delayed bleeding. A gastric wound model indicated that the hydrogels showed excellent treatment effects with significantly enhanced wound healing with type I collagen deposition, α-SMA expression, and blood vessel formation. These injectable self-healing adhesive hydrogels exhibited great potential to treat gastric wounds after endoscopic treatment.

scholarly journals Wound Healing Composite Materials of Bacterial Cellulose and Zinc Oxide Nanoparticles with Immobilized Betulin Diphosphate

Nanomaterials ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 713
Author(s):  
Nina Melnikova ◽  
Alexander Knyazev ◽  
Viktor Nikolskiy ◽  
Peter Peretyagin ◽  
Kseniia Belyaeva ◽  
...  
Keyword(s):  
Wound Healing ◽  
Zinc Oxide ◽  
Bacterial Cellulose ◽  
Zinc Oxide Nanoparticles ◽  
Wound Dressings ◽  
Oxide Nanoparticles ◽  
Burn Wound ◽  
Zno Nps ◽  
Wound Model ◽  
And Oxidative Stress

A design of new nanocomposites of bacterial cellulose (BC) and betulin diphosphate (BDP) pre-impregnated into the surface of zinc oxide nanoparticles (ZnO NPs) for the production of wound dressings is proposed. The sizes of crystalline BC and ZnO NPs (5–25%) corresponded to 5–6 nm and 10–18 nm, respectively (powder X-ray diffractometry (PXRD), Fourier-infrared (FTIR), ultraviolet (UV), atomic absorption (AAS) and photoluminescence (PL) spectroscopies). The biological activity of the wound dressings “BC-ZnO NPs-BDP” was investigated in rats using a burn wound model. Morpho-histological studies have shown that more intensive healing was observed during treatment with hydrophilic nanocomposites than the oleophilic standard (ZnO NPs-BDP oleogel; p < 0.001). Treatment by both hydrophilic and lipophilic agents led to increases in antioxidant enzyme activity (superoxide dismutase (SOD), catalase) in erythrocytes and decreases in the malondialdehyde (MDA) concentration by 7, 10 and 21 days (p < 0.001). The microcirculation index was restored on the 3rd day after burn under treatment with BC-ZnO NPs-BDP wound dressings. The results of effective wound healing with BC-ZnO NPs-BDP nanocomposites can be explained by the synergistic effect of all nanocomposite components, which regulate oxygenation and microcirculation, reducing hypoxia and oxidative stress in a burn wound.

Evaluation of Potential Application of Wharton’s Jelly-Derived Human Mesenchymal Stromal Cells and its Conditioned Media for Dermal Regeneration using Rat Wound Healing Model

2021 ◽  
pp. 1-14
Author(s):  
Caroline Mathen ◽  
Mrunal Ghag Sawant ◽  
Raghubansh Gupta ◽  
Wilfrid Dsouza ◽  
Shilpa G. Krishna
Keyword(s):  
Wound Healing ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Therapeutic Potential ◽  
Wound Care ◽  
Free Cell ◽  
Conditioned Media ◽  
Human Umbilical Cord ◽  
Human Mscs ◽  
Wound Model

Mesenchymal stromal cells and the derived conditioned media represent an area of tremendous medical interest and, among other clinical applications, are currently being extensively explored for wound healing. The aim of this study was to comparatively evaluate the wound healing potential of xeno-free human umbilical cord-derived mesenchymal stromal cells (MSCs) and the conditioned media (CM) in a full-thickness excision wound model in rats. The evaluation parameters included rate of wound healing, serum cytokine analyses, collagen content, histopathology, and hyperspectral imaging as an independent qualitative and quantitative tool. Both the cell-based and cell-free approaches scored better in lower inflammation, as evidenced in lower IL-10 and stable IL-6 levels, and improved rate of wound healing (<i>p</i> &#x3c; 0.0001). More importantly, no adverse reaction or rejection was observed although human MSCs and CM were used in a xenogeneic model. The presence of hFGF, hHGF, hGCSF, hIL-1Ra, hVEGF, and hIL-6 in the secretome may elucidate the regenerative potential of the xeno-free cell-based and cell-free approaches which have translational value for advanced wound care. The results revealed the therapeutic potential of both the cell-based and cell-free approaches for wound healing.

Development of a Three-Dimensional Human Skin Equivalent Wound Model for Investigating Novel Wound Healing Therapies

2010 ◽  
Vol 16 (5) ◽  
pp. 1111-1123 ◽  
Author(s):  
Yan Xie ◽  
Simone C. Rizzi ◽  
Rebecca Dawson ◽  
Emily Lynam ◽  
Sean Richards ◽  
...  
Keyword(s):  
Wound Healing ◽  
Human Skin ◽  
Three Dimensional ◽  
Skin Equivalent ◽  
Wound Model ◽  
Human Skin Equivalent

Effects of Carbon Arc Lamp Irradiation on Wound Healing in a Rat Cutaneous Full-Thickness Wound Model

2018 ◽  
Author(s):  
Jian-Xin Yan ◽  
Xuan Liao ◽  
Sheng-Hong Li ◽  
Hong-Wei Liu ◽  
Han-Yu Chang ◽  
...  
Keyword(s):  
Wound Healing ◽  
Full Thickness ◽  
Wound Model ◽  
Carbon Arc ◽  
Full Thickness Wound

scholarly journals An Equine Wound Model to Study Effects of Bacterial Aggregates on Wound Healing

2019 ◽  
Vol 8 (10) ◽  
pp. 487-498 ◽  
Author(s):  
Elin Jørgensen ◽  
Lene Bay ◽  
Lene T. Skovgaard ◽  
Thomas Bjarnsholt ◽  
Stine Jacobsen
Keyword(s):  
Wound Healing ◽  
Wound Model ◽  
Bacterial Aggregates

scholarly journals EFFECT OF HEALOL OIL ON WOUND HEALING IN WISTAR RATS: A NOVEL STUDY

2018 ◽  
Vol 11 (3) ◽  
pp. 152
Author(s):  
Sujith V ◽  
Poornima G ◽  
Balaji O ◽  
Bairy Kl ◽  
Praveen K ◽  
...  
Keyword(s):  
Wound Healing ◽  
Wistar Rats ◽  
Chronic Wounds ◽  
Statistical Significance ◽  
Silver Sulfadiazine ◽  
Histopathological Analysis ◽  
Burn Wound ◽  
Excision Wound ◽  
Burn Wounds ◽  
Wound Model

 Objective: Wound healing is a complex process, and various plant extracts have been used to study the effect of medicinal plants on wound healing. Healol oil is used in some tribal areas for ulcer healing and treatment of burn wounds. There are no animal studies done so far using healol oil to find out its role in the treatment of wounds. Hence, the aim of our study is to find the effect of healol oil in excision as well burn wounds in Wistar rats.Methods: Thirty-six Wistar rats were used, 18 rats in excision wound model and remaining in burn wound model. Each model consists of three groups of six rats each. Povidone-iodine was used as a standard control in excision wound model and silver sulfadiazine was used as a standard control in burn wound model. Healol oil was used as test drug in both the models. Period of epithelization and wound contracture rates were analyzed. Histopathological analysis of the skin tissue was done. One-way analysis of variance was used followed by Tukey’s post hoc test for statistical analysis using SPSS version 23 software, P value of <0.05 was taken as statistical significance.Results: Both standard and test groups showed significantly faster wound healing (p<0.001) compared to control rats treated with paraffin wax in both the models. In excision model standard povidone was significantly (p<0.001) better compared to healol oil whereas in burn model, silver sulfadiazine and healol oil showed comparable results with respect to period of epithelization without statistical significance (p>0.05).Conclusion: Wound healing property of healol oil is proved, and further clinical trial can be done to find out the effect of healol oil on chronic wounds due to various etiologies.

A Method for Quantitative Analysis of the Kinematics of Fibroblast Migration in a Monolayer Wound Model

2011 ◽  
Author(s):  
Gil Topman ◽  
Orna Sharabani-Yosef ◽  
Amit Gefen
Keyword(s):  
Wound Healing ◽  
Cell Migration ◽  
Wound Healing Assay ◽  
Complete Coverage ◽  
Time Intervals ◽  
Fibroblast Migration ◽  
Wound Model ◽  
Regular Time

A wound healing assay is simple but effective method to study cell migration in vitro. Cell migration in vitro was found to mimic migration in vivo to some extent [1,2]. In wound healing assays, a “wound” is created by either scraping or mechanically crushing cells in a monolayer, thereby forming a denuded area. Cells migrate into the denuded area to complete coverage, and thereby “heal” the wound. Micrographs at regular time intervals are captured during such experiments for analysis of the process of migration.

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