Polyhexanide as a new antiseptic composition for a dentist

Aim. To assess systematically the available scientific evidence about clinical response of using polyhexanide as an antiseptic agent in medicine and dentistry. The properties of polyhexanide – excellent antimicrobial activity, chemical stability and low toxicity – have been studied.

Prospects for use of the preparation «cyteal» in patients with periodontal pathology

he modern interpretation of the principles of therapy of periodontal pathology brings individualized treatment of patients to the first place. The basic principle of local therapy of periodontal diseases, in our opinion, is drug therapeutic treatment. The success of the treatment of periodontal diseases, the duration of remission and the prevention of complications largely depends on the quality of the impact on the oral cavity microflora. Recently, antiseptics of complex action have been successfully used. One of the representatives of the group of antiseptics is the drug «Citeal», which contains active substances: hexamidine, chorhexidine, chlorcresol. The purpose of the work is to increase the effectiveness of treatment of patients with periodontal pathology due to the use of three-component antiseptic agent of complex action — «Citeal». The results of a comprehensive examination of patients after the end of treatment show an improvement in oral hygiene, a decrease in the PMA index in comparison with the data before treatment. The Schiller-Pisarev test also became negative after the treatment. It is recommended to carry out professional hygiene with the use of antiseptic solution «Citeal» in a 1:10 dilution in the form of application to the marginal and alveolar part of the gums and irrigation of periodontal pockets, followed by mandatory rinsing of the mouth with water to remove residues. It was found that an individualized and differentiated approach to the treatment of patients with various forms of gingivitis and generalized periodontitis, who have orthopedic structures, prevents exacerbation of the disease during the year.

Pengaruh Paparan Gas Ozon terhadap Jumlah Koloni Jamur Candida albicans

Candida albicans is one of the pathogenic fungal species which causes oral infections. One of the methods to minimize the risk of fungal infection is by application of asepsis procedure through gargling with antiseptics. Ozone has high oxidative properties, therefore, it is considered as an antiseptic agent. Plasma Study Center of Diponegoro University has developed an ozone generator machine which opens up an opportunity to identify ozone properties as an antiseptic in controlling the number of fungal colonies, especially Candida albicans. This study was aimed to identify the effects of ozone exposure on the number of fungal colonies of Candida albicans. This was a laboratory experimental study with one-group pretest-posttest design. A total of 32 samples of Candida albicans suspensions were divided into two groups (16 each). Group 1 was not treated with ozonation, while group 2 were ozonated. Then, the two groups were cultured in SDA media using the spread plate method and were incubated for 48 hours. The data were obtained by calculating the number of fungal colonies growing in SDA media. The Wilcoxon test obtained a p-value <0.05, meaning that there were differences in the colony number of Candida albicans before and after ozonation. In conclusion, ozone exposure has a significant effect on the number of fungal colonies of Candida albicans. Further studies are needed adding variables or parameters. Keywords: ozone; fungal colony; Candida albicans Abstrak: Candida albicans merupakan salah satu spesies jamur patogen yang menyebabkan infeksi oral. Salah satu metode untuk meminimalkan risiko infeksi jamur ialah melalui penerapan prosedur asepsis dengan berkumur menggunakan antiseptik. Ozon memiliki kemampuan oksidasi tinggi sehingga dipertimbangkan untuk menjadi bahan antiseptik. Pusat Penelitian Plasma Universitas Diponegoro telah mengembangkan mesin generator ozon yang membuka peluang untuk mengetahui kemampuan ozon sebagai antiseptik dalam mengendalikan jumlah koloni jamur terutama Candida albicans. Penelitian ini bertujuan untuk mengetahui adanya pengaruh paparan gas ozon terhadap jumlah koloni jamur (Candida albicans). Jenis penelitian ialah eksperimental laboratorik dengan one group pretest-posttest design. Sebanyak 32 sampel suspensi jamur Candida albicans dibagi menjadi dua kelompok (masing-masing 16 sampel). Kelompok 1 tidak dilakukan ozonasi dan kelompok 2 dilakukan ozonasi kemudian kedua kelompok dikultur dalam media SDA dengan metode spread plate dan diinkubasi 48 jam. Data diperoleh dengan menghitung jumlah koloni jamur yang tumbuh pada media SDA. Hasil uji statistik Wilcoxon mendapatkan nilai p<0,05 yang berarti terdapat perbedaan jumlah koloni jamur (Candida albicans) sebelum dan setelah pemberian gas ozon. Simpuolan penelitian ini ialah paparan gas ozon berpengaruh secara bermakna terhadap jumlah koloni jamur (Candida albicans). Penelitian lanjutan diperlukan dengan penambahan variabel atau parameter.Kata kunci: gas ozon; koloni jamur; Candida albicans

Problematic issues of diagnosis and treatment of vulvovaginitis of mixed bacterial-candidiasis etiology

The aim of the study is to evaluate the effectiveness of modern antiseptic agent Gynodek in the treatment of mixed bacterial-candidiasis vulvovaginitis.Materials and methods. There were 67 patients with bacterial-candidiasis vulvovaginitis under observation. The diagnosis of candidiasis infection was verified according to the data of clinical and laboratory research methods. Patients received vaginal gel Gynodek 5 ml once a day for 7 days. The effectiveness of treatment was assessed according to the data of the clinical examination before treatment and on the 7th day of observation, and according to the laboratory (bacterioscopic, bacteriological) data before and after treatment. Complete clinical and bacterial debridement was criteria of the treatment effectiveness.Results and discussion. The clinical picture of bacterial-fungal vulvovaginitis was characterized by a decrease in the number and severity of symptoms such as hyperemia and edema of the mucous membrane, discharge from the genital tract was not typical.There were no complaints in 95.5% of the examined women after end of therapy, there were no objective symptoms in all women on the 7th day of treatment. Bacteriological examination after 2 weeks confirmed the therapy effectiveness in 86.6% of women. Peptostreptococcus spp. after treatment sowed at significant concentrations 106–107 CFU/ml only in 1.5% of patients, Streptococcus spp. in 8.9% of women, Candida albicans in 13.4%. Lactobacilli in the amount of 107 CFU/ml were in 86.6% of patients, which indicates a rapid restoration of normal vaginal microflora.Conclusions. Bacterial-fungal associations of microorganisms in the vaginal biotope lead to a complex clinical symptoms, which makes it difficult to make a timely diagnosis. Gynodek vaginal gel is highly effective against gram-positive, gram-negative and fungal microflora, which makes it the drug of choice in the treatment of bacterial-candidiasis vulvovaginitis. Gynodek creates a comfortable pH, promotes the rapid restoration of own lactoflora, and increases the functional activity of the vaginal mucosa epithelium, which ensures the prevention of vaginal infection recurrence.

Triclosan: Electrochemistry, Spontaneous Degradation and Effects on Double-Stranded DNA

Triclosan (TCS) is an antiseptic agent widely used mainly in personal care products and an important contaminant, which degrades in the environment causing toxicity on health, including negative effects on DNA. In this context, an electrochemical investigation of TCS in aqueous solution was studied by voltammetric techniques. The TCS underwent irreversible oxidation in a pH-dependent process, leading to the formation of two reversibly oxidized and pH-dependent oxidation products. An oxidation mechanism for TCS and its oxidation products in neutral aqueous medium was proposed. Besides that, the TCS spontaneously degraded into supporting eletrolytes with 3.4 ≤ pH ≤ 12.04 over the incubation time and the degraded TCS in solution was detected by electrochemical and spectrophotometric techniques. A higher degradation of TCS was observed in alkaline medium. In addition, the interaction in situ of this anti-microbial with DNA was investigated using dsDNA incubated solutions and dsDNA electrochemical bisosensor, by voltammetry. TCS and degraded TCS interacted with dsDNA causing the condensation of the double helix structure, release of guanine (by TCS and dedraded TCS) and adenine (by degraded TCS) bases from dsDNA and a possible intercalation of degraded TCS in the polynucleotide chain. No dsDNA oxidative damage was detected.

Povidone iodine hookah therapy for the treatment of COVID-19 and other respiratory virus: A hypothesis we must test

Povidone – Iodine has been known to have strong bactericidal and virucidal properties for a long time. It has been in clinical use as an antiseptic agent for over fifty years. Now a new indication for this old drug is recommended as Hookah therapy. It is hypothesized that in this form it will be an effective therapeutic agent to kill the coronavirus (SARS-COV2) or significantly inhibit its multiplication to achieve a cure. Details of treatment and dosage form are presented.

Gas embolism after periproctal abscess incision and lavage with hydrogen peroxide − a case report Should the use of hydrogen peroxide in surgery be continued?

Introduction: Hydrogen peroxide is an antiseptic solution still often used in surgical departments for lavage of wounds. Its use is nevertheless linked to an important risk of gas embolism. Such a case report has not yet been published in the Czech literature and awareness of this danger is low among surgeons. Case report: The author describes the case of gas embolism in a 40 years old patient after lavage of a periproctal abscess incision with 3% hydrogen peroxide. The lavage resulted in a cardiopulmonary arrest with the need of cardiopulmonary resuscitation. Fortunately, the patient recovered without any health consequences. Conclusion: In the view of important risks and questionable and insufficiently proven benefits it may be the time to possibly reconsider the use of hydrogen peroxide in surgery and replace it with a different antiseptic agent.

Comparative Study of Bacterial Growth Inhibitory Effect of Three Types of Mouthwashes Marketed in Japan Containing Povidone Iodine, Benzethonium Chloride, and Chlorhexidine Gluconate by Bacterial Culture

Purpose: Pathogenic microorganisms in saliva are thought to be a major cause of surgical site infection in patients who have undergone surgery for head and neck or upper digestive tract cancers, and a common cause of postoperative pneumonia and aspiration pneumonia in elderly people. Gargling with a mouthwash containing an antiseptic agent is recommended to control salivary pathogen populations, but it is not clear which antiseptic agents are most effective. The purpose of this study is to examine the potencies of three antiseptic mouthwashes marketed in Japan against salivary bacteria.

Pharmacokinetics Of Chitosan-Ethacridine Adduct In Rats

The objective of this study is to investigate the pharmacokinetics of the chitosan-ethacridine adduct in white rats. It was demonstrated that the kinetics of ethacridine release from adduct are significantly different from that for intraperitoneally introduced ethacridine. When free ethacridine was introduced, 80% of the administered amount was detected in blood of rats in 30 min. This quantity decreased gradually until 20 h, when 1.25% of the administered amount was detected. Contrary, for chitosan-ethacridine adduct, the ethacridine content in the blood slowly increased for 8 h and then decreased. Even after 72 h of the injection, 3% of the injected amount of ethacridine was detected. The experiment demonstrates a significant delay in the release of ethacridine from the chitosan-ethacridine adduct in the blood of treated rats. Thus, a possibility of using chitosan for prolongation of action of the ethacridine as an antiseptic agent was shown.