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Nature ◽  
2021 ◽  
Author(s):  
Andrew C. Yang ◽  
Fabian Kern ◽  
Patricia M. Losada ◽  
Maayan R. Agam ◽  
Christina A. Maat ◽  
...  

Nature ◽  
2021 ◽  
Author(s):  
Andrew C. Yang ◽  
Fabian Kern ◽  
Patricia M. Losada ◽  
Maayan R. Agam ◽  
Christina A. Maat ◽  
...  

Author(s):  
Andrew C. Yang ◽  
Fabian Kern ◽  
Patricia M. Losada ◽  
Christina A. Maat ◽  
Georges Schmartz ◽  
...  

AbstractThough SARS-CoV-2 primarily targets the respiratory system, it is increasingly appreciated that patients may suffer neurological symptoms of varied severity1–3. However, an unbiased understanding of the molecular processes across brain cell types that could contribute to these symptoms in COVID-19 patients is still missing. Here, we profile 47,678 droplet-based single-nucleus transcriptomes from the frontal cortex and choroid plexus across 10 non-viral, 4 COVID-19, and 1 influenza patient. We complement transcriptomic data with immunohistochemical staining for the presence of SARS-CoV-2. We find that all major cortex parenchymal and choroid plexus cell types are affected transcriptionally with COVID-19. This arises, in part, from SARS-CoV-2 infection of the cortical brain vasculature, meninges, and choroid plexus, stimulating increased inflammatory signaling into the brain. In parallel, peripheral immune cells infiltrate the brain, microglia activate programs mediating the phagocytosis of live neurons, and astrocytes dysregulate genes involved in neurotransmitter homeostasis. Among neurons, layer 2/3 excitatory neurons—evolutionarily expanded in humans4—show a specific downregulation of genes encoding major SNARE and synaptic vesicle components, predicting compromised synaptic transmission. These perturbations are not observed in terminal influenza. Many COVID-19 gene expression changes are shared with those in chronic brain disorders and reside in genetic variants associated with cognitive function, schizophrenia, and depression. Our findings and public dataset provide a molecular framework and new opportunities to understand COVID-19 related neurological disease.


2019 ◽  
Vol 39 (10) ◽  
pp. 1936-1948 ◽  
Author(s):  
Chi Gu ◽  
Xiaodi Hao ◽  
Jianru Li ◽  
Ya Hua ◽  
Richard F Keep ◽  
...  

Hydrocephalus has been reported to occur in spontaneous hypertensive rats (SHRs). The purposes of this study were (1) to use T2 magnetic resonance imaging to examine time of onset, (2) to elucidate potential underlying mechanisms and (3) to determine whether minocycline could prevent hydrocephalus development. Ventriculomegaly was evaluated by T2 imaging in SHRs and Wistar-Kyoto rats from weeks 4 to 7 after birth. Brain histology and transmission electron microscopy were used to assess the periventricular and choroid plexus damage. SHRs were also treated with either vehicle or minocycline. We found that hydrocephalus was observed in SHRs but not in Wistar-Kyoto rats. It occurred at seven weeks of age but was not present at four and five weeks. The hydrocephalus was associated with epiplexus cell (macrophage) activation, choroid plexus cell death and damage to the ventricle wall. Treatment with minocycline from week 5 attenuated hydrocephalus development and pathological changes in choroid plexus and ventricular wall at week 7. The current study found that spontaneous hydrocephalus arises at ∼7 weeks in male SHRs. The early development of hydrocephalus (persistent ventricular dilatation) may result from epiplexus cell activation, choroid plexus cell death and periventricular damage, which can be ameliorated by treatment with minocycline.


2018 ◽  
Vol 315 (3) ◽  
pp. C357-C366 ◽  
Author(s):  
Daniel Preston ◽  
Stefanie Simpson ◽  
Dan Halm ◽  
Alexandra Hochstetler ◽  
Christian Schwerk ◽  
...  

The choroid plexus (CP) epithelium plays a major role in the production of cerebrospinal fluid (CSF). A polarized cell line, the porcine CP-Riems (PCP-R) line, which exhibits many of the characteristics of the native epithelium, was used to study the effect of activation of the transient receptor potential vanilloid 4 (TRPV4) cation channel found in the PCP-R cells as well as in the native epithelium. Ussing-style electrophysiological experiments showed that activation of TRPV4 with a specific agonist, GSK1016790A, resulted in an immediate increase in both transepithelial ion flux and conductance. These changes were inhibited by either of two distinct antagonists, HC067047 or RN1734. The change in conductance was reversible and did not involve disruption of epithelial junctional complexes. Activation of TRPV4 results in Ca2+ influx, therefore, we examined whether the electrophysiological changes were the result of secondary activation of Ca2+-sensitive channels. PCP-R cells contain two Ca2+-activated K+ channels, the small conductance 2 (SK2) and the intermediate conductance (IK) channels. Based on inhibitor studies, the former is not involved in the TRPV4-mediated electrophysiological changes whereas one of the three isoforms of the IK channel (KCNN4c) may play a role in the apical secretion of K+. Blocking the activity of this IK isoform with TRAM34 inhibited the TRPV4-mediated change in net transepithelial ion flux and the increased conductance. These studies implicate TRPV4 as a hub protein in the control of CSF production through stimulation by multiple effectors resulting in transepithelial ion and subsequent water movement.


2016 ◽  
Vol 28 (9) ◽  
Author(s):  
A. R. Costa ◽  
H. Marcelino ◽  
I. Gonçalves ◽  
T. Quintela ◽  
J. Tomás ◽  
...  

2016 ◽  
Vol 65 (4) ◽  
pp. 295-302 ◽  
Author(s):  
Masaoki Takano ◽  
Mieko Otani ◽  
Takahiro Kaji ◽  
Keiji Sano ◽  
Michiko Hamada-Kanazawa ◽  
...  

2013 ◽  
Vol 54 (7) ◽  
pp. 4827 ◽  
Author(s):  
Yuan Lei ◽  
William D. Stamer ◽  
Jihong Wu ◽  
Xinghuai Sun

2008 ◽  
Vol 4 ◽  
pp. T247-T247
Author(s):  
Sandra Marisa Oliveira ◽  
Isabel Longo Alves ◽  
Diogo Trigo ◽  
Maria João Saraiva

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