Sex Hormones Protect Against Amyloid-β Induced Oxidative Stress in the Choroid Plexus Cell Line Z310

2016 ◽  
Vol 28 (9) ◽  
Author(s):  
A. R. Costa ◽  
H. Marcelino ◽  
I. Gonçalves ◽  
T. Quintela ◽  
J. Tomás ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Choroid Plexus ◽  
Cell Line ◽  
Sex Hormones ◽  
Amyloid Β ◽  
Plexus Cell

Serotonin, bradykinin and endothelin signalling in a sheep choroid plexus cell line

Life Sciences ◽  
1999 ◽  
Vol 64 (10) ◽  
pp. 859-867 ◽  
Author(s):  
Olivier Albert ◽  
Nicolas Ancellin ◽  
Laurence Preisser ◽  
Alain Morel ◽  
Bruno Corman
Keyword(s):  
Choroid Plexus ◽  
Cell Line ◽  
Plexus Cell

scholarly journals Activation of TRPV4 stimulates transepithelial ion flux in a porcine choroid plexus cell line

2018 ◽  
Vol 315 (3) ◽  
pp. C357-C366 ◽  
Author(s):  
Daniel Preston ◽  
Stefanie Simpson ◽  
Dan Halm ◽  
Alexandra Hochstetler ◽  
Christian Schwerk ◽  
...  
Keyword(s):  
Choroid Plexus ◽  
Cell Line ◽  
Transient Receptor Potential ◽  
Water Movement ◽  
Receptor Potential ◽  
Ion Flux ◽  
Transient Receptor Potential Vanilloid ◽  
Junctional Complexes ◽  
Plexus Cell ◽  
Transient Receptor

The choroid plexus (CP) epithelium plays a major role in the production of cerebrospinal fluid (CSF). A polarized cell line, the porcine CP-Riems (PCP-R) line, which exhibits many of the characteristics of the native epithelium, was used to study the effect of activation of the transient receptor potential vanilloid 4 (TRPV4) cation channel found in the PCP-R cells as well as in the native epithelium. Ussing-style electrophysiological experiments showed that activation of TRPV4 with a specific agonist, GSK1016790A, resulted in an immediate increase in both transepithelial ion flux and conductance. These changes were inhibited by either of two distinct antagonists, HC067047 or RN1734. The change in conductance was reversible and did not involve disruption of epithelial junctional complexes. Activation of TRPV4 results in Ca2+ influx, therefore, we examined whether the electrophysiological changes were the result of secondary activation of Ca2+-sensitive channels. PCP-R cells contain two Ca2+-activated K+ channels, the small conductance 2 (SK2) and the intermediate conductance (IK) channels. Based on inhibitor studies, the former is not involved in the TRPV4-mediated electrophysiological changes whereas one of the three isoforms of the IK channel (KCNN4c) may play a role in the apical secretion of K+. Blocking the activity of this IK isoform with TRAM34 inhibited the TRPV4-mediated change in net transepithelial ion flux and the increased conductance. These studies implicate TRPV4 as a hub protein in the control of CSF production through stimulation by multiple effectors resulting in transepithelial ion and subsequent water movement.

Vasopressin V1a Receptor Signaling in a Rat Choroid Plexus Cell Line

2000 ◽  
Vol 275 (2) ◽  
pp. 322-327 ◽  
Author(s):  
T. Battle ◽  
L. Preisser ◽  
V. Marteau ◽  
G. Meduri ◽  
M. Lambert ◽  
...  
Keyword(s):  
Choroid Plexus ◽  
Cell Line ◽  
Receptor Signaling ◽  
V1a Receptor ◽  
Plexus Cell ◽  
Vasopressin V1a Receptor

Proteomic analysis of mouse choroid plexus cell line ECPC-4 treated with lipid A

2016 ◽  
Vol 65 (4) ◽  
pp. 295-302 ◽  
Author(s):  
Masaoki Takano ◽  
Mieko Otani ◽  
Takahiro Kaji ◽  
Keiji Sano ◽  
Michiko Hamada-Kanazawa ◽  
...  
Keyword(s):  
Choroid Plexus ◽  
Cell Line ◽  
Proteomic Analysis ◽  
Lipid A ◽  
Plexus Cell

Lipopolysaccharide activates the kallikrein–kinin system in mouse choroid plexus cell line ECPC4

2008 ◽  
Vol 434 (3) ◽  
pp. 310-314 ◽  
Author(s):  
Masaoki Takano ◽  
Chieko Satoh ◽  
Naomi Kunimatsu ◽  
Mieko Otani ◽  
Michiko Hamada-Kanazawa ◽  
...  
Keyword(s):  
Choroid Plexus ◽  
Cell Line ◽  
Kinin System ◽  
Plexus Cell ◽  
Kallikrein Kinin System

Berberine nanoencapsulation attenuates hallmarks of scoplomine induced Alzheimer's-like disease in rats

2020 ◽  
Vol 15 ◽  
Author(s):  
Samar R. Saleh ◽  
Mariam M. Abady ◽  
Mohammed Nofal ◽  
Nashwa W. Yassa ◽  
Mohamed S. Abdel-latif ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Histopathological Examination ◽  
Memory Function ◽  
Amyloid Β ◽  
Active Pharmaceutical Ingredient ◽  
Isoquinoline Alkaloid ◽  
Drug Uptake ◽  
Male Rats ◽  
Morris Water Maze Test ◽  
Neuroprotective Effects

Background: Berberine (BBR), an isoquinoline alkaloid, acts as a multipotent active pharmaceutical ingredient to counteract several types of dementia based on its numerous pharmacological actions including antioxidant, antiinflammatory, cholesterol-lowering effect, and inhibition of Aβ production and AChE. However, BBR suffers from poor absorption, bioavailability and brain drug uptake. The present study is directed for the formulation and characterization of Chitosan BBR-nanoparticles (BBR-NPs) as well as the estimation of its neuroprotective effects against scopolamine induced cognitive impairments. Methods: BBR-NPs were formulated using ionic gelation method and tripolyphosphate was chosen as a cross linker. Nanoparticles size, zeta potential, encapsulation efficiency and releasing profile were estimated. To investigate the neuroprotective effects, adult fifty six Wistar male rats were randomly distributed into: three control groups, received saline, polyethylene glycol or chitosan- NPs respectively; induced group, received scopolamine (2 mg/ kg, i.p.) and three treated groups were orally administrated BBR (50 mg/ kg), BBR- NP (7 mg/ kg) and donepezil (2.25 mg/ kg, as positive control) followed by scopolamine injection after 40 min, daily for 4 weeks. Morris water maze test, oxidative stress parameters, cholinergic and amyloid-β processing intermediates as well as neuroplasticity markers and histopathological examination were assessed. Results: Our results showed that BBR- NPs were better than BBR and donepezil as BBR- NPs were powerful inhibitory ligands toward AChE and Aβ42 formation and significantly down regulated Tau, iNOS and BACE gene expression in rats’ hippocampus. BBR-NPs administration, at 1/6 of BBR therapeutic recommended dose, significantly improved learning and memory function. This could be accredited to the diminution of oxidative stress and amyloid-β toxicity in addition to the improvement of the neuroplasticity markers. Conclusions: The enhancing effect of BBR- NPs could be related to the enhancing of its bioavailability, absorption and brain drug uptake which need more investigation in future work.

Characterizing effects of excess copper levels in a human astrocytic cell line with focus on oxidative stress markers

2021 ◽  
Vol 65 ◽  
pp. 126711
Author(s):  
Barbara Witt ◽  
Michael Stiboller ◽  
Stefanie Raschke ◽  
Sharleen Friese ◽  
Franziska Ebert ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cell Line ◽  
Oxidative Stress Markers ◽  
Stress Markers ◽  
Excess Copper
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