The Rhizobium and calcium fertilizer application to peanut plant in dry land

One of the main problems of peanut planting in dry land is a lack of nitrogen and calcium availability. Efforts to increase the nitrogen and calcium availability for peanut farming in dry land is needed and one possible approach is by application of Rhizobium and calcium fertilizers. This research was conducted to determine the effect of the application of Rhizobium (Nodulin) and calcium fertilizers on growth and yield of peanut plants in dry land. Two experiments were conducted, one in a greenhouse using a completely randomized design and another experiment in the field using a randomized block design. The treatments were with the application of Nodulin, without Nodulin, with calcium fertilizer and without calcium fertilizer. The results showed that the application of calcium and Nodulin was able to increase the number of root nodules and peanut growth. Nodulin and calcium fertilizers increased the dry pod weight of 2.177 g per 6 m2 or 3.7 tons.ha-1. The application of Nodulin and calcium was also able to increase the nitrogen and calcium content of leaf tissue up to 18.9% and 20.9%, respectively.

Calcium Affects Polyphosphate and Lipid Accumulation in Mucoromycota Fungi

Calcium controls important processes in fungal metabolism, such as hyphae growth, cell wall synthesis, and stress tolerance. Recently, it was reported that calcium affects polyphosphate and lipid accumulation in fungi. The purpose of this study was to assess the effect of calcium on the accumulation of lipids and polyphosphate for six oleaginous Mucoromycota fungi grown under different phosphorus/pH conditions. A Duetz microtiter plate system (Duetz MTPS) was used for the cultivation. The compositional profile of the microbial biomass was recorded using Fourier-transform infrared spectroscopy, the high throughput screening extension (FTIR-HTS). Lipid content and fatty acid profiles were determined using gas chromatography (GC). Cellular phosphorus was determined using assay-based UV-Vis spectroscopy, and accumulated phosphates were characterized using solid-state 31P nuclear magnetic resonance spectroscopy. Glucose consumption was estimated by FTIR-attenuated total reflection (FTIR-ATR). Overall, the data indicated that calcium availability enhances polyphosphate accumulation in Mucoromycota fungi, while calcium deficiency increases lipid production, especially under acidic conditions (pH 2–3) caused by the phosphorus limitation. In addition, it was observed that under acidic conditions, calcium deficiency leads to increase in carotenoid production. It can be concluded that calcium availability can be used as an optimization parameter in fungal fermentation processes to enhance the production of lipids or polyphosphates.

Have Sustained Acidic Deposition Decreases Led to Increased Calcium Availability in Recovering Watersheds of the Adirondack Region of New York, USA?

Soil calcium depletion has been strongly linked to acidic deposition in eastern North America and recent studies have begun to document the recovery of soils in response to large decreases in acidic deposition. However, increased calcium availability has not yet been seen in the B horizon, where calcium depletion has been most acute, but mineral weathering is critically important for resupplying ecosystem calcium. This study provides new data in seven watersheds in the Adirondack region (New York, USA), where acidic deposition impacts on soils and surface waters have been substantial and recovery remains slow. Initial sampling in 1997–1998 and 2003–2004 was repeated in 2009–2010, 2014, 2016 and 2017. Exchangeable calcium concentrations increased by an average of 43% in the Oe horizon of three watersheds where this horizon was sampled (10.7–15.3 cmolc kg−1). Changes in calcium were not seen in the individual watersheds of the Oa and B horizons, but as a group, a significant increase in calcium was measured in the upper B horizon. Liming of a calcium-depleted watershed also tripled calcium concentration in the upper B horizon in 5 years. However, stream calcium in unlimed watersheds decreased over the study period. Small increases in B-horizon calcium may be underway.

Function of Large and Small Platelets Differs, Depending on Extracellular Calcium Availability and Type of Inductor

It is widely anticipated that large platelets are more reactive than small platelets. This was mainly shown in Ca2+-poor media albeit extracellular Ca2+ is utilized by platelets for activation. We determined the impact of extracellular Ca2+ on functional differences between large and small platelets in response to thrombin receptor activating peptide 6 (TRAP-6), adenosine diphosphate (ADP), and epinephrine. In Ca2+-poor buffer, large platelets responded stronger to TRAP-6 which equalized in Ca2+ containing buffer. Large platelets contained and mobilized more Ca2+ from their intracellular stores upon TRAP-6 stimulation explaining their better reactivity in Ca2+-poor media. Stronger aggregation of large platelets in response to ADP also equalized in presence of Ca2+, whereas large platelets responded weaker to ADP in flow cytometry (CD62P-expression: 9.7 mean fluorescence intensity [MFI] [4.4–17.9] vs. 17.5 MFI [6.1–45.6], p = 0.0234) and PAC-1 binding (11.1 MFI [5.7–19.6] vs. 20.5 MFI [14.4–35.0], p = 0.0078). Epinephrine response was stronger in large platelets (CD62P-expression: 11.8 MFI [6.8–33.0] vs. 6.8 MFI [2.5–15.2], p = 0.0078; PAC-1 binding 18.9 MFI [13.6–38.4] vs. 13.0 MFI [6.8–22.4], p = 0.0234; max. aggregation 82.9% [58.7–94.8] vs. 77.2% [19.8–88.8], p = 0.0313), which expressed more α2A receptors. Epinephrine further increased phosphatidylserine (PS) exposure especially in large platelets. PS-positive platelets progressively divided into two subpopulations with high or basic intracellular Ca2+ dependent on extracellular Ca2+. Thrombin generation was faster with small, but accelerated by PS exposure and epinephrine-coactivated large platelets. We show that responses of large and small platelets differ depending on extracellular Ca2+ availability and the inductor. Careful control of extracellular Ca2+ is necessary in functional studies with large and small platelets.