pheromone bioassay
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1971 ◽  
Vol 61 (2) ◽  
pp. 283-291 ◽  
Author(s):  
Phyllis L. Robertson ◽  
C. J. Orton

Techniques and apparatus developed for pheromone bioassay studies of ants are described. They include a ventilated cage, with a blacked-out nest area and a two-compartment arena, in which colonies are maintained in the laboratory, and in which group experiments are carried out; a prey treatment apparatus, in which a stinging ant is clamped at the junction of head and thorax, with its body supported on a length of black nylon tubing which is used as artificial prey after it has been stung for a specified period; an applicator for introducing into the cage artificial prey which have been treated with ant secretions, either by the latter method, or by the direct application of dissected gland material; and a closed-system, pheromone-testing apparatus, in which an ant is held in a perspex observation chamber with controlled lighting, supplied with air at constant temperature, relative humidity and flow rate, and treated with gland odours introduced into the conditioned air.


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