Macro and Microanatomical Structures of Lizard’s Regenerate Tail (Mabouya multifasciata Kuhl, 1820)

Lizards are animals that can do autotomy. Research on the autotomy of lizard’s tail has been carried out, but few have examined about axial skeletons that focus on vertebrae caudales or arranged muscles. That is the background of this research. This research is expected to be a database for further research and as a comparison between animals that can perform other autotomies. This research intend to determine the macro and micro structures of the anatomical axial skeleton lizard’s regenerate tail and to know the micro structures of anatomy muscular segmentation lizard’s regenerate tail. The used methods are X-Ray, Alizarin Red S and Alcian Blue, Paraffin method with Hematoxylin-Eosin and Mallory Triple Strain staining. The result showed that the lizard's regenerate tail was darker than the original. On observations using X-Ray and Alizarin staining showed that the original tail is seen broad and have a Processus. The original lizard's tail is composed of bones, because it is red which shows perfectly calcified bones. While the regenerate of the lizard's tail is composed of bones in the form of long pipes which are red-colored because they have calcified from outside. Muscular segmentation of the original lizard's tail when viewed transversely has irregular segments. While the lizard's regenerate tail has 15 muscle segments. Muscles are composed of a collection of myotubes that become myotomes, each of myotomes confined by the myoseptum.

Alizarin Red S-Alcian Blue Staining for Regenerated tail of Common House Gecko (Hemidactylus frenatus)

Common House Gecko (Hemidactylus frenatus) is one of reptiles that have ability to autotomy their tails. Tail autotomy is a mechanism to protect it self from predators. After the tail broke, there will be wound healing on the tail which is then followed by a tail regeneration event. Original tail and regenerate tail is very different morphologically and anatomically. The original tail is composed of bones while the tail of the regenerate is composed of cartilage. Histochemical staining using Alizarin Red-S Alcian Blue was done to differentiate bone and cartilage. This method will stained bones red while the cartilage will stained blue.

Histological Study of Common House Gecko (Hemidactylus frenatus) Regenerated Tail

Common house gecko<strong> </strong>(<em>Hemidactylus frenatus</em>) belongs to suborder Lacertilia that has capacity to shed its tail (autotomy) as a self-defense mechanism. After autotomy, tail regeneration occurs. Axiale skeleton of an original tail is composed of bony vertebrae, whereas the the regenerate one is comprised of cartilaginous tube. The purpose of this study was to determine the histological difference between axial skeleton of the original tail and the regenerate one of the common house gecko. Twenty four individuals consist of twelve common house gecko with original tail and twelve with regenerate tail were used. Microanatomical observations were carried on histological slide of original and regenerated tail stained with Hematoxylin-Eosin and Mallory Acid Fuchsin. The results showed that the original tail comprised of bony vertebrae whereas regenerated tail supported by cartilaginous tube.