scholarly journals Macro and Microanatomical Structures of Lizard’s Regenerate Tail (Mabouya multifasciata Kuhl, 1820)

2019 ◽  
Vol 2 ◽  
pp. 25-29
Author(s):  
Ardiana Ardiana ◽  
Muhammad Ja’far Luthfi ◽  
Nyoman Puniawati Soesilo ◽  
Laila Ainun Nisa ◽  
Risti Widyaningsih
Keyword(s):  
Alcian Blue ◽  
Axial Skeleton ◽  
Alizarin Red S ◽  
Alizarin Red ◽  
X Ray ◽  
Regenerate Tail ◽  
Micro Structures ◽  
Hematoxylin Eosin ◽  
Paraffin Method

Lizards are animals that can do autotomy. Research on the autotomy of lizard’s tail has been carried out, but few have examined about axial skeletons that focus on vertebrae caudales or arranged muscles. That is the background of this research. This research is expected to be a database for further research and as a comparison between animals that can perform other autotomies. This research intend to determine the macro and micro structures of the anatomical axial skeleton lizard’s regenerate tail and to know the micro structures of anatomy muscular segmentation lizard’s regenerate tail. The used methods are X-Ray, Alizarin Red S and Alcian Blue, Paraffin method with Hematoxylin-Eosin and Mallory Triple Strain staining. The result showed that the lizard's regenerate tail was darker than the original. On observations using X-Ray and Alizarin staining showed that the original tail is seen broad and have a Processus. The original lizard's tail is composed of bones, because it is red which shows perfectly calcified bones. While the regenerate of the lizard's tail is composed of bones in the form of long pipes which are red-colored because they have calcified from outside. Muscular segmentation of the original lizard's tail when viewed transversely has irregular segments. While the lizard's regenerate tail has 15 muscle segments. Muscles are composed of a collection of myotubes that become myotomes, each of myotomes confined by the myoseptum.

scholarly journals Calcification of Axial Skeleton and Segmentation Muscular Regeneration of Gecko’s Tail (Gekko Gecko Linnaeus, 1758)

2019 ◽  
Vol 2 ◽  
pp. 189-192
Author(s):  
Azkiya Zahrotus Syarifah ◽  
Muhammad Ja’far Luthfi ◽  
Alfiatun Wa’is Khusnul Khatimah ◽  
Aulya Nidaur Rahmah
Keyword(s):  
Data Base ◽  
Axial Skeleton ◽  
Blue Color ◽  
Alizarin Red ◽  
Gekko Gecko ◽  
X Ray ◽  
Tail Autotomy ◽  
Hematoxylin Eosin ◽  
Paraffin Method ◽  
Muscular Regeneration

Gecko is an animal that can carry out autotomy. Research on gecko tail autotomy has been carried out, but there are still few who research the axial skeleton that focuses on vertebrae caudales and segmentation muscular arranged, this is the background of this research. This research is expected to be data base further research and as a comparison between animals that can induce further autotomy. This study aims to determine the macrostructure and microstructure of axial axial gecko tail regenerates and determine the anatomical microstructure of muscular regeneration of gecko tail. The methods used were X-Ray, Alizarin Red S and Alcian Blue, Paraffin method with hematoxylin-eosin staining, and Mallory Triple Stain. The results showed that the color of the gecko tail regenerate was paler compared to the original tail. On observations using radiological rays and alizarin staining showed that the original tail wo uld look segmented and have a process. The original gecko tail is composed of bones, because it is red which shows perfectly calcified bone. While the gecko tail regenerate is composed of cartilage that is shaped like a long pipe glazed red because it has calcified. At the end of the tail there is also a blue color, this indicates that the gecko tail regenerate has not been completely calcified. Segmentation Muscular of the original gecko tail, when viewed longitudinally, indicates a segment that extends from one process to the skin and when viewed from the cross has only four muscle segments separated by the septum. While the gecko tail regenerate when viewed in a longitudinal manner there is no segment and when seen transversely there are 12 muscle segments seen. Muscles are composed of a collection of myotubes that form myotomes, each myotomes limited by myoseptum.

scholarly journals Alizarin Red S-Alcian Blue Staining for Regenerated tail of Common House Gecko (Hemidactylus frenatus)

2018 ◽  
Vol 7 (2) ◽  
pp. 57-59
Author(s):  
Rakhmiyati Rakhmiyati ◽  
Muhammad Ja’far Luthfi
Keyword(s):  
Alcian Blue ◽  
Histochemical Staining ◽  
Alizarin Red S ◽  
Blue Staining ◽  
Alcian Blue Staining ◽  
Tail Regeneration ◽  
Alizarin Red ◽  
Regenerate Tail ◽  
Hemidactylus Frenatus ◽  
Common House

Common House Gecko (Hemidactylus frenatus) is one of reptiles that have ability to autotomy their tails. Tail autotomy is a mechanism to protect it self from predators. After the tail broke, there will be wound healing on the tail which is then followed by a tail regeneration event. Original tail and regenerate tail is very different morphologically and anatomically. The original tail is composed of bones while the tail of the regenerate is composed of cartilage. Histochemical staining using Alizarin Red-S Alcian Blue was done to differentiate bone and cartilage. This method will stained bones red while the cartilage will stained blue.

Double staining technique for rat foetus skeletons in teratological studies

1979 ◽  
Vol 13 (4) ◽  
pp. 309-310 ◽  
Author(s):  
J. Whitaker ◽  
Kathleen M. Dix
Keyword(s):  
Alcian Blue ◽  
Staining Technique ◽  
Alizarin Red S ◽  
Double Staining ◽  
Alizarin Red

Cartilage and bone were differentiated using alcian blue and alizarin red S respectively. Anomalies of both cartilaginous and bony parts of the skeleton could be examined.

scholarly journals Μελέτη έκφρασης γονιδίων αρχέγονων βλαστικών κυττάρων σε τροφοβλαστικά κύτταρα

2016 ◽  
Author(s):  
Ελένη Κατσιάνη
Keyword(s):  
Alcian Blue ◽  
Alizarin Red S ◽  
Cresyl Violet ◽  
Rt Pcr ◽  
Alizarin Red ◽  
Hla Dr

ΕισαγωγήΠαραδοσιακά τα μεσεγχυματικά βλαστικά κύτταρα (MSCs) μπορούν να προκύψουν από πληθώρα ανθρώπινων ιστών. Πρόσφατα, MSCs απομονώθηκαν από εξωεμβρυϊκές πηγές όπως το ομφαλικό αίμα και η γέλη του Wharton, οι αμνιακές μεμβράνες, το αμνιακό υγρό και ο πλακούντας. ΜSCs προερχόμενα από πλακουντιακές χοριακές λάχνες πρώτου τριμήνου φαίνεται να ομοιάζουν, βιολογικά, στα εμβρυϊκά βλαστικά κύτταρα (ESCs), λόγω του πρώιμου σταδίου ανάπτυξης του πλακούντα.Στην παρούσα μελέτη, προσπαθήσαμε να διαλευκάνουμε τα κοινά χαρακτηριστικά μεταξύ των MSCs προερχόμενα από πρώτου τριμήνου χοριακές λάχνες και ESC. Με βάση τα δεδομένα προηγούμενων μελετών, πραγματοποιήθηκαν παρατεταμένες χρονικά καλλιέργειες των MSCs με σκοπό να αξιολογηθούν in vitro, πως διαμορφώνονται το δυναμικό διαφοροποίησης, τα μοριακά χαρακτηριστικά και γενικότερα οι δείκτες πολυδυναμοκότητας καθόλη τη περίοδο καλλιέργειας.Υλικά και ΜέθοδοιMSCs ελήφθησαν από 30 δείγματα χοριακών λαχνών και καλλιεργήθηκαν για να εκτιμηθεί η ικανότητα πολλαπλασιασμού τους και τα μορφολογικά χαρακτηριστικά. Κυτταρομετρία ροής χρησιμοποιήθηκε για να προσδιοριστεί η έκφραση των αντιγόνων επιφανείας των MSCs. Τα MSCs διαφοροποιήθηκαν σε λιποκύτταρα, οστεοβλάστες, χονδροκύτταρα και νευρικά κύτταρα. Μέσω RT-PCR ανάλυθηκε η έκφραση των γονιδίων-δεικτών των ESC και των γονιδίων δεικτών της διαφοροποίησης.ΑποτελέσματαΤα χοριακά λαχνικά κύτταρα, αναπτύσσονται εύκολα, είναι φαινοτυπικά σταθερά με χαρακτηριστική ινοβλαστοειδή μορφολογία. MSCs ανακαλλιεργήθηκαν για 20 φορές κατά τη διάρκεια περιόδου 120 ημερών. Ρυθμός πολλαπλασιασμού των MSCs παρέμενε σταθερός στα πρώιμα στάδια (3η-12η ανακαλλιέργεια) και παρουσίασε πτώση στα μεταγενέστερα στάδια (13η-20η ανακαλλιέργεια). Καθόλη τη διάρκεια της παρατεταμένης χρονικά καλλιέργειας ο καρυότυπος των κυττάρων δεν τροποποιηθήκε.Η ανάλυση κυτταρομετρίας ροής έδειξε ότι τα κύτταρα χοριακών λαχνών ήταν θετικά για CD90, CD73, CD105, CD29, CD44, HLA ABC αντιγόνα και αρνητικά για CD14, CD34, AC133, και HLA DR αντιγόνα, για ολόκληρη την περίοδο καλλιέργειας.Τα από χοριακές λάχνες προερχόμενα MSCs διαφοροποιήθηκαν επιτυχώς σε λιποκύτταρα, οστεοβλάστες, χονδροκύτταρα αλλά και σε νευρικά κύτταρα. Διαφοροποίηση επιβεβαιώθηκε με χρώσεις Oil Red Ο, Alizarin Red S, Alcian Blue και Cresyl Violet αντίστοιχα και έκφραση των γονιδίων PPARγ2, SPP1, Sox9 και Νεστίνης αντίστοιχα.Η έκφραση ESCs γονιδίων-δεικτών POU5F1 (Οct-4) και Nanog παρατηρήθηκε σε πρώιμα στάδια (4-12 περάσματα), ενώ τα τελευταία στάδια (14-20 περάσματα) δεν παρουσίασαν ανιχνεύσιμα επίπεδα έκφρασης. ZFP42 και Sox2 έκφραση δεν παρατηρήθηκε. Επιπλέον, MSCs βρέθηκαν να εκφράζουν το γονίδιο GATA4 αλλά όχι το NES (νεστίνη) σε μη διαφοροποιημένα κύτταρα.ΣυμπεράσματαΤα από χοριακές λάχνες προερχόμενα MSCs έχουν πολυδύναμες ιδιότητες μεταξύ εμβρυϊκών και ενήλικων βλαστικών κύτταρα. Στην παρούσα μελέτη με τη χρήση ενός ευρύτερου φάσματος δεικτών, διαπιστώθηκε ότι MSC από χοριακές λάχνες εμφανίζουν υψηλό ρυθμό πολλαπλασιασμού, διατηρώντας αναλλοίωτο το χρωμοσωμικό τους προφίλ, και την αυτό-ανανεωτική ικανότητα τους, μοιράζονται κοινή έκφραση αντιγόνων επιφάνειας με τα ενήλικα MSCs και εκφράζουν ορισμένα γονίδια μάρτυρες των εμβρυϊκών βλαστικών κυττάρων. Αυτά τα χαρακτηριστικά θέτουν τις χοριακές λάχνες ως μια ελκυστική πηγή MSC για τις ανάγκες της αναγεννητικής ιατρικής.

scholarly journals Modified Alizarin Red S-Alcian Blue Staining for Reptilian Skeleton

2016 ◽  
Vol 5 (1) ◽  
pp. 19
Author(s):  
Muhammad Ja’far Luthfi
Keyword(s):  
Alcian Blue ◽  
Anatomical Study ◽  
Alizarin Red S ◽  
Blue Staining ◽  
Alcian Blue Staining ◽  
Double Staining ◽  
Alizarin Red ◽  
Important Method ◽  
Clearing Method ◽  
And Cartilage

<p>Skeletal staining is an important method in anatomical study. The aim of the research was to develop staining and clearing method of Reptilian skeleton using Alizarin Red S-Alcian Blue. The specimen were eviscerated, fixed, stained, cleared, and keep in glycerine solution. This modified double-staining has successfully stain bone and cartilage of Reptilian.</p>

scholarly journals Hierarchical approach towards adsorptive removal of Alizarin Red S dye using native chitosan and its successively modified versions

2020 ◽  
Vol 82 (4) ◽  
pp. 715-731
Author(s):  
M. A. Khapre ◽  
R. M. Jugade
Keyword(s):  
Alizarin Red S ◽  
Point Of Zero Charge ◽  
Isotherm Models ◽  
X Ray Diffraction ◽  
Adsorption Method ◽  
Alizarin Red ◽  
Kinetics Of Adsorption ◽  
X Ray ◽  
Column Adsorption ◽  
Adsorption Isotherm Models

Abstract In the present work, native chitosan (Ch) along with its chemically and physico-chemically modified versions, namely sulphate cross-linked chitosan (SCC) and sulphate cross-linked chitosan–bentonite composite (SCC-B), were employed as potential adsorbents for the removal of an anionic dye, Alizarin Red S (ARS) from aqueous solutions. All three adsorbents were extensively characterized using techniques such as Fourier-transform infrared spectroscopy, scanning electron microscopy, energy dispersive X-ray, X-ray diffraction, Brunauer–Emmett–Teller analysis, thermogravimetric–differential thermal analysis, and pH point of zero charge. Various parameters were optimized, including pH of dye solution, contact time, adsorbent dose, initial adsorbate concentration and temperature of adsorption. Four adsorption isotherm models were studied and it was found that the Freundlich model was best-fit for all three systems. Maximum adsorption capacities towards adsorption of ARS were found to be 42.48, 109.12 and 131.58 mg g−1 for Ch, SCC and SCC-B, respectively. Kinetics of adsorption was examined by employing three well-known models in order to deduce the mechanism of adsorption. Thermodynamic studies show that the process is spontaneous and exothermic for all adsorbents employed. Furthermore, it was observed that for large sample volumes, the column adsorption method was more effective compared to the batch method.

scholarly journals Anatomical Study of Red-eared TurteTail (Trachemys scripta elegans)

2019 ◽  
Vol 2 ◽  
pp. 137-140
Author(s):  
Putri Nofita ◽  
Muhammad Ja’far Luthfi ◽  
Reza Sukma Dewi
Keyword(s):  
Anatomical Study ◽  
Slow Motion ◽  
Axial Skeleton ◽  
Trachemys Scripta ◽  
Histological Structure ◽  
Fat Tissue ◽  
Trachemys Scripta Elegans ◽  
Alizarin Red ◽  
First Time ◽  
Hematoxylin Eosin

Turtles are reptiles armored backs hard, slow motion, appearing for the first time about 200 million years ago and relative ly unchanged for 150 million years. This study aims to determine the anatomical structure of the axial skeleton and determine the histologic structure of red-eared turtles (Trachemys scripta elegans) females. The method used, among others, Alizarin Red S staining Alcian Blue, methods of paraffin with Hematoxylin-eosin staining (HE) and Mallory acid fuchsin and used 3 adult turtles 3 years old. The results showed that vertebral caudalis red-eared turtles (Trachemys scripta elegans) females composed of true bone, vertebrae-type amphicoelous not have plains autotomy and histology red-eared turtles (Trachemys scripta elegans) females resemble histological structure other tail reptiles with some differences that only have 4 files do not have the muscle and fat tissue.

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