Early stage of nucleic acid electrochemistry. Detection of DNA damage in X-ray-irradiated rats

First papers on electroactivity of DNA and RNA were published more then 50 years ago. For about 8 years oscillographic polarography at controlled a.c. (OP, proposed by J. Heyrovský already in 1941) was the method of choice for DNA analysis. Since approximately 1954 Robert Kalvoda developed OP for wide application in various fields. It is shown that already before 1960 it was possible to detect damage to DNA in X-ray-irradiated rats by means of OP. DNA samples from irradiated animals produced significantly larger OP anodic guanine signal indicating changes in the DNA structure. At present, radiation-induced strand breaks and damage to bases in DNA can be electrochemically detected at high sensitivity.

Determination of Trace Manganese in the Source Water by Oscillographic Polarography

Manganese in water has low toxicity. The product will form the yellow stain when the industrial water content is high. There are many ways to monitor it. The determination of trace Manganese in the YangjiaoPu source water by oscillographic polarography is introduced in this paper. The result indicate that the standard deviation of this method is 0.001~0.002 mg/L, the relative standard deviation is 2.3~2.5% and the mark-on recovery ranged from 96.8%～105.8%. At the same time several problems needing attention about experiment process are posed in this paper.