Does environmental enrichment improve performance, morphometry, yield and weight of broiler parts at different ages?

ABSTRACT The inclusion of environmental enrichment in conventional broiler rearing can increase mobility, bone mass and muscle. This research aimed to evaluate the use of environmental enrichment in the rearing of broilers at different ages and its influence on performance, morphometry, yield and weight of the parts. It was used the completely randomized design in split-plot scheme, being the plots the treatments presence (T1) and absence (T2) of environmental enrichment and the subplots the broiler ages (1, 7, 14, 21, 28, 35 and 42 days), with 56 chicks in each treatment (T1 and T2). Data obtained were analyzed by the linear effect model of fixed effects and compared by Tukey’s test of means. The animals were raised in a controlled environment, divided into four boxes with dimensions: 1.5 × 1.0 × 0.7 m, containing rice straw bed. In T1 a ladder with a perch on top was used, distributed every 1.5 m2. Environmental enrichment used did not influence broiler’s zootechnical performance. Broilers’ morphometric properties, parts weight and body weight increased due to environmental enrichment and, improvement was observed in chicks rearing’s final phase. The environmental enrichment was beneficial for muscle and bone mass gain in the main commercial parts of the chicken carcass, in addition to reducing the allometric coefficient of the breasts in chickens.

Kajian Cemaran Salmonella spp pada Daging Ayam Broiler di Pasar Tradisional dan Modern Kota Bandar Lampung

Food can be a carrier for pathogenic agents that can disease in consumers (foodborne disease). Chicken meat is one of the products from livestock which has a high consumption rate, because besides being easy to obtain, the growth of chickens is fast, and the price is also more affordable compared to large livestock types. Chicken meat is an excellent medium for microbial growth and makes it a perishable food ingredient. Foodborne illness is a disease caused by pathogenic microorganisms that contaminate food, such as Salmonella spp, Staphylococcus aureus, and Escherichia coli (E.coli). Salmonella spp infection can cause salmonellosis which irritates the digestive tract and many of them result in death. This study aims to assess the presence and number of pathogenic microorganisms Salmonella spp in broiler chicken sold in traditional and modern markets in Bandar Lampung. The research was conducted by purposive sampling of 30 chicken meat samples. Broiler chicken carcass samples were taken as whole and then stored in sterile plastic, labeled and put into a cool box filled with ice. The variables observed in this study were the presence and number of Salmonella bacteria. The data obtained were analyzed descriptively. The results of Salmonella spp examination on chicken meat samples in traditional markets in Bandar Lampung City showed a positive in 1 (one) sample or 6.7%, while in modern markets Salmonella was not found. The presence of Salmonella in chicken meat is thought to be due to contamination from the water used during the process of slaughtering and preparing the carcass, contamination from the carcass slaughtering and selling environment which does not apply good sanitary hygiene and poor personal hygiene of the traders. The conclusion obtained is that the number of Salmonella spp in chicken meat in traditional markets is not in accordance with SNI 7388 of 2009 concerning the Maximum Limit of Microbial Contamination (BMCM) in fresh meat.

Non-invasive Monitoring of Antioxidants and Stress in Captive Indian Leopards

Background: Captive Indian leopards are exposed to different kind of stresses part of which can be alleviated through supplementation of carotenoids. However, invasive monitoring of antioxidants is often pro-act as a stressor itself. Hence, use of non-invasive monitoring of antioxidants and stress would be desirable. However, before recommendation, such non-invasive procedures must be compared with conventional ones. Hence, this experiment was designed to compare the cortisol and total antioxidants (TAA) status measured either in serum or faecal samples. Methods: Three diets were tested on twelve adult leopards (7 M and 5 F, BW ranging from 45-63 kg) in an experiment based on replicated Latin square design comprising of three treatments, three periods and three sequences and four animals in each sequence. The ratio of buffalo meat: chicken carcass was 100:0, 90:10 and 80:10 in groups CON, GI and GII, respectively. Result: Intake and absolute quantity of carotenoids absorbed increased (P less than 0.01) with increased level of chicken carcass; however, efficiency of absorption was lower (P less than 0.05) in GII as compared to other two groups. Faecal concentrations of cortisol decreased (P less than 0.0001) and TAA increased (P less than 0.0001) with increased level of carotenoids in the diet. Irrespective of the dietary treatments, concentration of cortisol was lower (P less than 0.0001) and TAA was higher in faecal as compared to serum samples. Regression analysis revealed positive relationship between serum and faecal sample assay with respect to both TAA and cortisol. Thus, faecal samples could be used to monitor cortisol and antioxidant status in Indian leopards. Assay of faecal samples indicated that replacement of buffalo meat with chicken at 20% in the diet improved the antioxidants and alleviated stress in captive leopards.

A novel radiographic grid aiming device for percutaneous renal puncture

Background This in-vitro study aims to assess the efficacy of a novel radiographic grid aiming device as compared to the conventional “bull’s eye” puncture technique for percutaneous renal puncture. Methods This new device uses two radiopaque rings for targeting, somewhat similar to the concept of gun sights. The device is designed to improve the ease and accuracy of the “bull’s eye” puncture technique. We compared this device to the conventional “bull’s eye” puncture technique using a porcine kidney inside a chicken carcass as the study model. Two first-year residents used both the radiographic grid aiming device in turn, Group A, and the conventional bull’s eye technique, Group B, to perform punctures to reach the target porcine calyces in order. Related data were recorded and analyzed. Results 23 renal calyceal punctures were performed per group. There were no statistical differences in the overall puncture success rate, puncture time, or radiation exposure time and dose between the two groups. The number of punctures required to achieve success was higher in group B and the distance between the puncture site to the center of papillary fornix was shorter in group A, all P < 0.05. Conclusions The novel radiographic grid aiming device showed no advantage in the overall success rate for renal puncture. However, it offered the advantages of more precise puncture with fewer puncture attempts. It may serve as a useful tool for training. It may also offer some benefit to surgeons who are novices at percutaneous renal puncture.

Pseudomonas aeruginosa infection of avian origin: Zoonosis and one health implications

Zoonotic diseases are diseases that are transmitted from animals to humans and vice versa. Pseudomonas aeruginosa (P. aeruginosa) is a pathogen with zoonotic nature. Commercial poultry could be infected with P. aeruginosa, especially at young ages with great losses. Infection of embryos with P. aeruginosa induced death in the shell, while infection of chicks led to septicemia, respiratory and enteric infections, and high mortality. Humans are also highly susceptible to P. aeruginosa infection, and the disease is associated with severe lung damage, especially in immunocompromised patients. Chicken carcass and related poultry retail products play an important role in the transmission of P. aeruginosa to humans, especially after processing in abattoirs. Treatment of P. aeruginosa infection is extremely difficult due to continuous development of antibiotic resistance. The transfer of antibiotic-resistant genes from poultry products to humans creates an additional public health problem. Accordingly, this study focused on avian pseudomonad, especially P. aeruginosa, with respect to infection of poultry, transmission to humans, and treatment and antibiotic resistance.

An Investigation into the Critical Factors Influencing the Spread of Campylobacter during Chicken Handling in Commercial Kitchens in China

Campylobacteriosis is the most common cause of bacterial gastroenteritis worldwide. Consumption of chicken meat is considered the main route for human infection with Campylobacter. This study aimed to determine the critical factors for Campylobacter cross-contamination in Chinese commercial kitchens during chicken handling. Five commercial kitchens were visited to detect Campylobacter occurrence from 2019 to 2020. Chicken samples (n = 363) and cotton balls from the kitchen surfaces (n = 479) were collected, and total bacterial counts and Campylobacter spp. were detected. Genotypic characterization of 57 Campylobacter jejuni isolates was performed by multilocus sequence typing (MLST). In total, 77.41% of chicken carcass samples and 37.37% of kitchen surfaces showed Campylobacter spp. contamination. Before chicken preparation, Campylobacter spp. were already present in the kitchen environment; however, chicken handling significantly increased Campylobacter spp. prevalence (p < 0.05). After cleaning, boards, hands, and knives still showed high bacterial loads including Campylobacter spp., which related to poor sanitary conditions and ineffective handling practices. Poor sanitation conditions on kitchen surfaces offer greater opportunities for Campylobacter transmission. Molecular typing by MLST revealed that Campylobacter cross-contamination occurred during chicken preparation. The most prevalent sequence types, ST693 and ST45, showed strong biofilm formation ability. Consequently, sanitary condition of surfaces and biofilm formation ability of isolates were the critical points contributing to spread of Campylobacter in kitchen environment. These results provide insight into potential targeted control strategies along the farm-to-plate chain and highlight the necessity for improvements in sanitary conditions. The implementation of more effective cleaning measures should be considered to decrease the campylobacteriosis risk.

Kualitas Karkas Ayam Broiler yang Diberikan Pakan Supplemen Limbah Wortel

Carrot waste is carrots with criteria that are not suitable for human consumption. Carrot waste contains various vitamins and minerals. This study aims to evaluate the quality of broiler chicken carcass. The study used a completely randomized design (CRD) with 4 treatments and 6 replications. The treatments given included R0 = 0% additional feed from carrot waste; R1 = 0.5% additional feed waste carrots; R2 = 1% additional feed from carrot waste; R3 = 1.5% additional feed from carrot waste. The data obtained were tested for normality and homogeneity before being analyzed using the Analysis of Variants. If there is a difference then proceed with Duncan's multiple distance test. The results showed that the best results were given additional feed of carrot waste at the level of 1% to increase carcass weight and carcass percentage, carcass color would increase along with the increase in the level of carrot waste addition.

Main causes of chicken carcass condemnations in Espírito Santo, Brazil

Brazil is the third largest producer and the main exporter of chicken meat in the world. In 2019, it produced approximately 13.245 million tons of the product, generating more than six million dollars. According to the Poultry Association of the state of Espírito Santo, broiler production is carried out by 28 independent producers/companies, three integrator businesses, 42 integrated producers and seven slaughterhouses (structures with SIF, SIE/SISBI or SIE certification). Despite the growth of this sector, problems persist regarding the quality of carcasses and the number of condemnations. From the sanitary and industrial point of view, all products of animal origin must undergo prior inspection. The present study was developed using data generated by the Federal Inspection Service (Serviço de Inspeção Federal, SIF) and the State Inspection Service (Serviço de Inspeção Estadual, SIE). The main causes of condemnation of birds as inspected by SIF represented 9.26% of the slaughtered birds. Total condemnations corresponded to 1.35%. Overall, this study recorded a greater number of condemnations of non-pathological origin. In conclusion, there was consistency in the reasons why these products were condemned. Bruises/fractures and contamination were the most frequent causes according to both inspection services (SIF and SIE), indicating a common problem on the slaughter and inspection lines.

The use of audio-visual media in improving Culinary students learning outcomes in Chicken Carcass material

This research aimed to find out the effectiveness of audio-visual media (picture, video, audio) on culinary students’ learning outcomes in Chicken Carcass material. The researcher implemented a quasi-experimental method with a nonequivalent control group design. The subjects of this study were the 10th-grade students of SMK Negeri 8 Medan in the Academic Year of 2016/2017. Cluster random sampling was applied in this study, which consisted of one experimental class and one control class. Meanwhile, some hypothetical tests used in this study were n-gain, independent t-test, and dependent t-test. The n-gain score of learning outcomes in the experimental class is higher than the control class, which is 0.5 and categorized as moderate. Students’ learning outcomes between those who were taught using audio-visual media and without audio-visual media in Chicken Carcass material indicated a significant difference with a significance score of 0.000 ≤ 0.05. The audio-visual media effectively improve the learning outcomes of the 10th-grade students of the culinary department in Chicken Carcass material.