brain tissue section
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2021 ◽  
Vol 1142 ◽  
pp. 38-47
Author(s):  
Erteng Jia ◽  
Ying Zhou ◽  
Huajuan Shi ◽  
Min Pan ◽  
Xiangwei Zhao ◽  
...  

The Analyst ◽  
2021 ◽  
Author(s):  
Ibrahim Kaya ◽  
Eva Jennische ◽  
Stefan Lange ◽  
Per Malmberg

ToF-SIMS and/or MALDI-ToF imaging mass spectrometry of a single brain tissue section followed by classical- or immuno- histochemical staining.


Author(s):  
Hülya Torun ◽  
Buse Bilgin ◽  
Kemal Baysal ◽  
İbrahim Kulaç ◽  
İhsan Solaroğlu ◽  
...  

2019 ◽  
Author(s):  
Christopher Kune ◽  
Andréa Mc Cann ◽  
Raphaël La Rocca ◽  
Anthony Arguelles Arias ◽  
Mathieu Tiquet ◽  
...  

<div> <p>Kendrick mass defect (KMD) analysis is widely used for helping the detection and identification of chemically related compounds based on exact mass measurements. We report here the use of KMD as a criterion for filtering complex mass spectrometry dataset. The method enables an automated, easy and efficient data processing, enabling the reconstruction of 2D distributions of family of homologous compounds from MSI images. We show that the KMD filtering, based on an in-house software, is suitable and robust for high resolution (full width at half-maximum, FWHM, at <i>m/z</i> 410 of 20 000) and very high-resolution (FWHM, at <i>m/z</i> 410 of 160 000) MSI data. This method has been successfully applied to two different types of samples, bacteria co-cultures and brain tissue section</p> </div>


2019 ◽  
Author(s):  
Christopher Kune ◽  
Andréa Mc Cann ◽  
Raphaël La Rocca ◽  
Anthony Arguelles Arias ◽  
Mathieu Tiquet ◽  
...  

<div> <p>Kendrick mass defect (KMD) analysis is widely used for helping the detection and identification of chemically related compounds based on exact mass measurements. We report here the use of KMD as a criterion for filtering complex mass spectrometry dataset. The method enables an automated, easy and efficient data processing, enabling the reconstruction of 2D distributions of family of homologous compounds from MSI images. We show that the KMD filtering, based on an in-house software, is suitable and robust for high resolution (full width at half-maximum, FWHM, at <i>m/z</i> 410 of 20 000) and very high-resolution (FWHM, at <i>m/z</i> 410 of 160 000) MSI data. This method has been successfully applied to two different types of samples, bacteria co-cultures and brain tissue section</p> </div>


2018 ◽  
Author(s):  
Mayeul Collot ◽  
Pichandi Ashokkumar ◽  
Halina Anton ◽  
Emmanuel Boutant ◽  
Orestis Faklaris ◽  
...  

AbstractThe proper staining of the plasma membrane (PM) is critical in bioimaging as it delimits the cell. Herein, we developed MemBright: a family of six cyanine-based fluorescent turn-on PM probes that emit from orange to near-infrared when reaching the PM, and enable homogeneous and selective PM staining with excellent contrast in mono and two-photon microscopy. These probes are compatible with long-term live cell imaging and immunostaining. Moreover, MemBright label neurons in a brighter manner than surrounding cells allowing identification of neurons in acute brain tissue section and neuromuscular-junctions without any use of transfection or transgenic animals. At last, MemBright were used in super-resolution imaging to unravel the dendritic spines’ neck. 3D multicolor dSTORM in combination with immunostaining revealed en-passant synapse displaying endogenous glutamate receptors clustered at the axonal-dendritic contact site. MemBright probes thus constitute a universal toolkit for cell biology and neuroscience biomembrane imaging with a variety of microscopy techniques.


1993 ◽  
Vol 41 (4) ◽  
pp. 489-498 ◽  
Author(s):  
A Trembleau ◽  
D Roche ◽  
A Calas

We describe here a simple method for combining non-radioactive and radioactive in situ hybridization and immunohistochemistry on the same brain tissue section. This approach was first developed on the well-characterized hypothalamo-neurohypophyseal system, facilitating the optimization of the triple-labeling procedure and the verification of labeling specificity. We report the simultaneous detection of vasopressin (VP) mRNA with a digoxigenin-labeled oligonucleotide, oxytocin (OT) mRNA with a 35S-labeled oligonucleotide, and OT peptide in the same 12-microns cryostat section. This was performed on floating sections as follows: first, the two probes were hybridized simultaneously; second, the peptide was detected with an immunoperoxidase-DAB procedure; third, the digoxigenin-labeled probe was detected with an alkaline phosphatase-NBT/BCIP technique; and finally, the 35S-labeled probe was detected by histological autoradiography. We also demonstrate that this approach is suitable for the simultaneous detection of tyrosine hydroxylase and two less abundant mRNAs, vasoactive intestinal peptide and vasopressin mRNAs, in the suprachiasmatic nucleus. The combination of the three techniques did not significantly diminish their specificity or sensitivity. In conclusion, this new method, permitting the simultaneous detection of three different products of gene expression in the same section, could be useful for further analysis of the phenotypic organization and its plasticity in endocrine or neural tissues.


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