INTERACTION OF THROMBOLYTIC PROTEINASES COMPLEX LONGOLITIN AND ITS PRODUCER — MYCELIAL FUNGUS ARTHROBOTRYS LONGA — WITH ANTITHROMBOTIC DRUG PIYAVIT

Цель исследования: изучение взаимодействия комплекса протеолитических ферментов препарата лонголитин из анаморфного гриба Arthrobotrys longa с антикоагулянтным и антитромботическим препаратом пиявитом. Материалы и методы. Препарат пиявит смешивали в разных разведениях с различными концентрациями лонголитина и определяли фибринолитическую активность (ФА) смеси. В другой серии опытов пиявит добавляли в погруженную культуру гриба-продуцента. По окончании эксперимента в пробах культуральной жидкости (КЖ) определяли ФА. Антикоагулянтные свойства КЖ измеряли методом рекальцификации и записи тромбоэластограммы при добавлении КЖ к плазме крови здоровых интактных крыс. Результаты. В опытах in vitro пиявит дозозависимо угнетал ФА лонголитина до полного ее подавления при концентрации 16 мг/мл. Добавление пиявита к культуре гриба-продуцента вызывало усиление биосинтеза препарата с увеличением ФА. КЖ при этом обладала высокой антикоагулянтной активностью. Время рекальцификации и показатель R тромбоэластограммы в КЖ были статистически достоверно удлинены, увеличивалась и антиполимеризационная активность КЖ. Заключение. Совместное применение двух антитромботических средства (лонголитина и пиявита) в исследовании in vitro привело к уменьшению фибринолитических, а значит, и тромболитических свойств лонголитина. Таким образом, одновременное использование этих препаратов требует осторожности. Aim: to study the interaction of Longolitin (complex of proteolytic enzymes from anamorphic fungus Arthrobotrys longa) with anticoagulative and antithrombotic drug Piyavit. Materials and methods. We mixed diff erent dilutions of Piyavit with various concentrations of Longolitin and determined fibrinolytic activity (FA) of the mixture. In other experimental series we added Piyavit to submerged culture of the fungus-producer. At the end of the experiment we determined FA in culture liquid samples (CL). Anticoagulative properties of CL were measured by recalcification method and thromboelastograms recording with CL addition to blood plasma of healthy intact rats. Results. In in vitro experiments Piyavit dose-dependently inhibited FA of Longolitin until it’s completely suppression at concentration of 16 mg/ml. Piyavit addition to the culture of fungus-producer caused the enhancement of drug biosynthesis with FA increasing. At the same time CL had a high anticoagulative activity. Recalcifi cation time and parameter R of thromboelastogram in CL were statistically signifi cant elongated, also CL anti-polymerization activity increased. Conclusion. Combined application of two antithrombotic agents (Longolitin and Piyavit) in in vitro study led to decreasing of fibrinolytic, and so thrombolytic properties of Longolitine. Thus, the simultaneous use of these drugs requires caution. Adding piyavit to the culture of the producer fungus led to an increase in the biosynthesis of the preparation. Thus, caution is needed at simultaneous using of these drugs.

Dictyosporium wuyiense sp. nov. from Wuyi Mountain China

Dictyosporium wuyiense, a new anamorphic fungus collected on a dead branch of bamboo in Tongmugou, Wuyi Mountain, China, is described and illustrated. It is characterised by being cheiroid, septate, complanate, and yellow, with two types of conidia composed mostly of five parallel compact rows of cells, sometimes with variable hyaline apical vesical appendages. Type 1 conidia are longer and the three central rows are protruding with swollen apical cells; type 2 conidia are wider and fan-shaped, with rows of apical cells approximately the same length. The conidial morphology on potato dextrose agar plates was variable, most with 2–3 vesical appendages. In phylogenetic analyses of the internal transcribed spacer (ITS) of rDNA, Dictyosporium wuyiense clustered with most Dictyosporium species in Dictyosporiaceae, but was a distinct species. Both morphological and molecular evidence support its description as a new species. A brief review of Dictyosporium and allied species is provided.

Fusarium merismoides Corda. - A New Record of Anamorphic Fungus for Bangladesh

not availableJournal of Bangladesh Academy of Sciences, Vol. 40, No. 2, 207-209, 2016

Association of Pestalotiopsis Guepinii (DESM.) Stay. with Bipolaris Leaf Blight (BpLB) Infected Wheat Leaves-A new Record

Pestalotiopsis guepinii (Desm.) Stay an anamorphic fungus belonging to the class Celomycetes was isolated from Bipolaris leaf blight (BpLB) infected wheat leaf of two wheat varieties namely, Balaka and Saurab during the period of February to March, 2011. Association of Pestalotiopsis guepinii with wheat is a new record.Journal of Bangladesh Academy of Sciences, Vol. 40, No. 1, 87-90, 2016

Isaria takamizusanensis is the anamorph of Cordyceps ryogamimontana, warranting a new combination, Purpureocillium takamizusanense comb. nov.

The entomogenous anamorphic fungus Isaria takamizusanensis has not been resolved clearly in its teleomorphic state. We succeeded in inducing ascostroma formation by incubating conidiomata of I. takamizusanensis on cicada adults in a moist chamber. We observed the ascostroma and conducted a phylogenetic analysis based on ITS rDNA and EF-1α genes. The morphology of the ascostroma was identical to that of Cordyceps ryogamimontana. In the phylogenetic tree inferred from EF-1α, the isolate from the partspores grouped with nine strains derived from conidia of I. takamizusanensis, which was distinct from a clade including Purpureocillium lilacinum. Moreover, a conidial structure identical to that of I. takamizusanensis was rediscovered on the holotype specimen of C. ryogamimontana. As a result, we propose a new name, Purpureocillium takamizusanense, which is a combination of the teleomorph–anamorph connection of C. ryogamimontana–I. takamizusanensis, in accordance with the ‘one fungus, one name’ concept of the International Code of Nomenclature for Algae, Fungi, and Plants (ICN).