The Therapeutic Potential of the Essential Oil of Thymbra capitata (L.) Cav., Origanum dictamnus L. and Salvia fruticosa Mill. And a Case of Plant-Based Pharmaceutical Development

This review performs a comprehensive assessment of the therapeutic potential of three native herbs of Crete (Thymbra capitata (L.) Cav., Salvia fruticosa Mill. and Origanum dictamnus L.), their phytochemical constituents, health benefits and issues relevant to their safety, within a translational context. Issues discussed comprise: 1) Ethnopharmacological uses of the three herbs, reviewed through an extensive search of the literature; 2) Systematic analysis of the major phytochemical constituents of each plant, and their medicinal properties; 3) To what extent could the existing medicinal properties be combined and produce an additive or synergistic effect; 4) Possible safety issues. We conclude with a specific example of the use of a combination of the essential oils of these plants as an effective anti-viral product and the experience gained in a case of a plant-based pharmaceutical development, by presenting the major steps and the continuum of the translational chain.

Social Bacteriophages

Despite their simplicity, viruses can display social-like interactions such as cooperation, communication, and cheating. Focusing on bacteriophages, here we review features including viral product sharing, cooperative evasion of antiviral defenses, prudent host exploitation, superinfection exclusion, and inter-phage peptide-mediated signaling. We argue that, in order to achieve a better understanding of these processes, their mechanisms of action need to be considered in the context of social evolution theory, paying special attention to key population-level factors such as genetic relatedness and spatial structure.

A Viral Product Diffusion Model to Forecast the Market Performance of Products

To investigate the diffusion of products in the market, this paper proposes a viral product diffusion model using an epidemiological approach. This model presents the process of product diffusion through the dynamics of human behaviors. Based on the stability theory of Ordinary Differential Equations, we demonstrate the conditions under which a product in the market persists or dies out eventually. Next, we use Google data to validate the model. Fitting results illustrate that the viral product diffusion model not only depicts the steady growth process of products, but also describes the whole diffusion process during which the products increase at the initial stage and then gradually decrease and sometimes even exhibit multiple peaks. This shows that the viral product diffusion model can be used to forecast the developing tendency of products in the market through early behavior of these products. Moreover, our model also provides useful insights on how to design effective marketing strategies via social contagions.

Designing Viral Product Features for Broader Reach

Companies increasingly rely on “network” and “viral” marketing within their communication strategies. This study showed that providing viral products with specific features can increase their diffusion substantially. Products that were enabled to send automated notifications within a user’s local Facebook network upon adoption generated a 450 % higher adoption rate among Facebook friends compared with products without any viral features. Products that enabled adopters to send personal invitations to install the app generated an increase in the adoption rate by friends by 750 % more than in the control group. Although each personalized referral had a much stronger impact, notifications outperformed invitations in overall adoption. Automated notifications require no effort, and therefore substantially more messages were generated. The number of users who took the effort to send out personalized invitations was much smaller. A simulation of adoption beyond immediate individual networks showed that the passive-broadcast app experienced a 246 % increase in the rate of adoption, whereas adding active-personalized viral messaging capabilities generated only an additional 98 % increase, compared with the group without viral features.

Purification and characterization of FBI-1, a cellular factor that binds to the human immunodeficiency virus type 1 inducer of short transcripts.

The human immunodeficiency virus (HIV-1) promoter directs the synthesis of two classes of RNA molecules, short transcripts and full-length transcripts. The synthesis of short transcripts depends on a bipartite DNA element, the inducer of short transcripts (IST), located in large part downstream of the HIV-1 start site of transcription. IST does not require any viral product for function and is thought to direct the assembly of transcription complexes that are incapable of efficient elongation. Nothing is known, however, about the biochemical mechanisms that mediate IST function. Here, we report the identification and purification of a factor that binds specifically to the IST. This factor, FBI-1, recognizes a large bipartite binding site that coincides with the bipartite IST element. It is constituted at least in part by an 86-kDa polypeptide that can be specifically cross-linked to IST. FBI-1 also binds to promoter and attenuation regions of a number of cellular and viral transcription units that are regulated by a transcription elongation block. This observation, together with the observation that the binding of FBI-1 to IST mutants correlates with the ability of these mutants to direct IST function, suggests that FBI-1 may be involved in the establishment of abortive transcription complexes.