Zona pellucida in the process of fertilization and mammal embryonal development

In mammals, oocytes, fertilized eggs and pre-implantation embryos are surrounded by an acellular zona pellucida (zona pellucida – ZP). This structure has a fibro-spongy character but it undergoes constant modifications throughout its existence depending on many internal and external factors. ZP consists of glycoproteins marked as ZP1, ZP2, ZP3 and ZP4, the presence of which is species different. ZP1 and probably ZP4 molecules stabilize the fibrillary skeleton of the zona pellucida formed of ZP2 and ZP3 protein polymers which are ligands for specific spermatozoid receptors. The oligosaccharide chains of ZP3 are responsible for the primary attachment of the male gamete which induces the acrosomal reaction. ZP2 enhances this connection by secondary binding to an acrosome-free spermatozoid. Additionally, oviductal specific glycoprotein 1 which plays a role in interspecific oocyte-sperm interactions, appears around the postovulatory oocyte surrounded by ZP. In addition, this protein modifies the resistance of ZP to the action of proteases released as a result of the cortical reaction during polyspermia block. After fertilization, ZP not only protects the egg and then the embryo until implantation, but also has an embryotrophic effect. Understanding the molecular basics of the structure and properties of ZP can significantly improve animal fertility as well as reproductive rates.

Odorant and Taste Receptors in Sperm Chemotaxis and Cryopreservation: Roles and Implications in Sperm Capacitation, Motility and Fertility

Sperm chemotaxis, which guide sperm toward oocyte, is tightly associated with sperm capacitation, motility, and fertility. However, the molecular mechanism of sperm chemotaxis is not known. Reproductive odorant and taste receptors, belong to G-protein-coupled receptors (GPCR) super-family, cause an increase in intracellular Ca2+ concentration which is pre-requisite for sperm capacitation and acrosomal reaction, and result in sperm hyperpolarization and increase motility through activation of Ca2+-dependent Cl¯ channels. Recently, odorant receptors (ORs) in olfactory transduction pathway were thought to be associated with post-thaw sperm motility, freeze tolerance or freezability and cryo-capacitation-like change during cryopreservation. Investigation of the roles of odorant and taste receptors (TRs) is important for our understanding of the freeze tolerance or freezability mechanism and improve the motility and fertility of post-thaw sperm. Here, we reviewed the roles, mode of action, impact of odorant and taste receptors on sperm chemotaxis and post-thaw sperm quality.

CDC42 drives RHOA activity and actin polymerization during capacitation

Mammalian sperm cells acquire fertilizing capacity as a result of a process termed capacitation. Actin polymerization is important for capacitation; inhibiting actin polymerization prevents the adhesion and fusion of the sperm with the ovule. The main function of RHO proteins CDC42 and RHOA is to direct actin polymerization. Although these two RHO proteins are present in mammalian sperm, little is known about their role in capacitation, the acrosome reaction, and the way in which they direct actin polymerization. The purpose of this study was to determine the participation of CDC42 and RHOA in capacitation and the acrosome reaction and their relationship with actin polymerization using guinea pig sperm. Our results show that the inhibition of CDC42 and RHOA alters the kinetics of actin polymerization, capacitation, and the acrosome reaction in different ways. Our results also show that the initiation of actin polymerization and RHOA activation depend on the activation of CDC42 and that RHOA starts its activity and effect on actin polymerization when CDC42 reaches its maximum activity. Given that the inhibition of ROCK1 failed to prevent the acrosomal reaction, the participation of RHOA in capacitation and the acrosomal reaction is independent of its kinase 1 (ROCK1). In general, our results indicate that CDC42 and RHOA have different roles in capacitation and acrosomal reaction processes and that CDC42 plays a preeminent role.

The Effect of Sperm Preparation Medium on Malondialdehyde Concentration in the Seminal Plasma of Normozoospermic and Oligoastheno- teratozoospermic patient

Intra uterine insemination (IUI) has been widely used to treat infertility. Infertility may be due to increased levels of reactive oxygen species (ROS) in the seminal plasma. The goal of our study was to confirm that using sperm preparation medium to activate the spermatozoa in IUI might be helpful to decrease the levels of ROS in the seminal plasma, lowering the oxidative stress and the damage effects of the sperm and enhance a good quality sperm capable of capacitation, acrosomal reaction and fertilization. The levels of malondialdehyde (MDA) were measured in the seminal plasma in 40 infertile men of normozoospermia and oligoastheno-teratozoospermia before and after adding medium to the samples. Results showed that there was a significant (P<0.05) decrease in all parameters of semen samples in oligoastheno-teratozoosperia as compared with the other group, there was no significant increase in the level of MDA before adding the medium to the samples and a significant (P<0.05) increase after adding the medium to the same samples in the second group as compared with normozoospermic patients. Results reported in this study, clearly indicated that adding sperm preparation medium to the semen samples would improve the fertilization rates by decreasing the oxidative damages to the spermatozoa. Our results might be need a further studies to find a clue for the treatment of infertility in man.