scholarly journals Human spermatozoa possess a calcium-dependent chloride channel that may participate in the acrosomal reaction

2012 ◽  
Vol 590 (11) ◽  
pp. 2659-2675 ◽  
Author(s):  
Gerardo Orta ◽  
Gonzalo Ferreira ◽  
Omar José ◽  
Claudia L. Treviño ◽  
Carmen Beltrán ◽  
...  
Keyword(s):  
Chloride Channel ◽  
Human Spermatozoa ◽  
Calcium Dependent ◽  
Acrosomal Reaction

Calmodulin‐dependent activation of the epithelial calcium‐dependent chloride channel TMEM16A

2010 ◽  
Vol 25 (3) ◽  
pp. 1058-1068 ◽  
Author(s):  
Yuemin Tian ◽  
Patthara Kongsuphol ◽  
Martin Hug ◽  
Jiraporn Ousingsawat ◽  
Ralph Witzgall ◽  
...  
Keyword(s):  
Chloride Channel ◽  
Calcium Dependent

scholarly journals Extracellular protons enable activation of the calcium-dependent chloride channel TMEM16A

2017 ◽  
Vol 595 (5) ◽  
pp. 1515-1531 ◽  
Author(s):  
Silvia Cruz-Rangel ◽  
José J. De Jesús-Pérez ◽  
Iván A. Aréchiga-Figueroa ◽  
Aldo A. Rodríguez-Menchaca ◽  
Patricia Pérez-Cornejo ◽  
...  
Keyword(s):  
Chloride Channel ◽  
Calcium Dependent

scholarly journals Microbial Quorum-Sensing Molecules Induce Acrosome Loss and Cell Death in Human Spermatozoa

2009 ◽  
Vol 77 (11) ◽  
pp. 4990-4997 ◽  
Author(s):  
Claudia Rennemeier ◽  
Torsten Frambach ◽  
Florian Hennicke ◽  
Johannes Dietl ◽  
Peter Staib
Keyword(s):  
Quorum Sensing ◽  
Communication Systems ◽  
Homoserine Lactone ◽  
Human Spermatozoa ◽  
Dependent Manner ◽  
Pathogenic Yeast ◽  
Calcium Dependent ◽  
Opportunistic Pathogenic ◽  
Apoptosis And Necrosis ◽  
Dose Dependent

ABSTRACT Infertility in men and women is frequently associated with genital contamination by various commensal or uropathogenic microbes. Since many microorganisms are known to release quorum-sensing signals in substantial amounts, we raised the question whether such molecules can directly affect human spermatozoa. Here we show that farnesol and 3-oxododecanoyl-l-homoserine lactone, employed by the opportunistic pathogenic yeast Candida albicans and the gram-negative bacterium Pseudomonas aeruginosa, respectively, induce multiple damage in spermatozoa. A reduction in the motility of spermatozoa coincided in a dose-dependent manner with apoptosis and necrosis at concentrations which were nondeleterious for dendritic cell-like immune cells. Moreover, sublethal doses of both signaling molecules induced premature loss of the acrosome, a cap-like structure of the sperm head which is essential for fertilization. Addressing their mechanism of action, we found that the bacterial molecule, but not the fungal molecule, actively induced the acrosome reaction via a calcium-dependent mechanism. This work uncovers a new facet in the interaction of microorganisms with human gametes and suggests a putative link between microbial communication systems and host infertility.

scholarly journals ANO1 - a Candidate for Angiotensin-II-Activated Calcium Dependent Chloride Channel in Human Atrial Fibroblasts

2014 ◽  
Vol 106 (2) ◽  
pp. 560a
Author(s):  
Antoun El Chemaly ◽  
Caroline Norez ◽  
Christophe Magaud ◽  
Aurelien Chatelier ◽  
Patrick Bois
Keyword(s):  
Angiotensin Ii ◽  
Chloride Channel ◽  
Calcium Dependent

scholarly journals Proper cytoskeleton α‐tubulin distribution is concomitant to tyrosine phosphorylation during in vitro capacitation and acrosomal reaction in human spermatozoa

Cytoskeleton ◽  
2020 ◽  
Vol 77 (9) ◽  
pp. 333-341
Author(s):  
Paula Sáez‐Espinosa ◽  
Mariola Ferrández‐Rives ◽  
Natalia Huerta‐Retamal ◽  
Laura Robles‐Gómez ◽  
Jon Aizpurua ◽  
...  
Keyword(s):  
Tyrosine Phosphorylation ◽  
Human Spermatozoa ◽  
Acrosomal Reaction

Effect of temperature, nutrients, calcium, and cAMP on motility of human spermatozoa

1982 ◽  
Vol 242 (5) ◽  
pp. C304-C311 ◽  
Author(s):  
F. K. Gorus ◽  
R. Finsy ◽  
D. G. Pipeleers
Keyword(s):  
Seminal Fluid ◽  
Gradient Centrifugation ◽  
Cell Movement ◽  
Human Sperm ◽  
Correlation Spectroscopy ◽  
Human Spermatozoa ◽  
Effect Of Temperature ◽  
Dependent Manner ◽  
Calcium Dependent ◽  
Progressive Motility

The motility of human spermatozoa and its regulation were examined on cells isolated from other seminal components and purified into fractions of uniform progressive motility. The percent motile cells and estimates of their translational speed were determined by visual inspection, by stroboscopy, and by photon correlation spectroscopy; microcinematography and gradient centrifugation were occasionally used to clarify discrepancies. The motility of isolated spermatozoa could be maintained for periods up to 24 h at 4 or 37 degrees C; the presence of seminal fluid was not required and even provoked a reversible inhibition at 4 degrees C. Albumin facilitated cell movement between microscopic glass plates but had no effect on progressive motility per se, as evidenced by other techniques. During incubations of up to 2 h, progressive cell motility occurred independently of extracellular glucose and calcium but responded to variations in adenosine 3',5'-cyclic monophosphate and calcium. Dibutyryl cAMP increased forward motility, whereas ethyleneglycol-bis(beta-aminoethylether)-N,N'-tetraacetic acid reversibly immobilized the spermatozoa in a calcium-dependent manner; phosphodiesterase inhibition resulted in increased vibration of sperm heads without any effect on progressive motility. Longer incubation periods required the presence of extracellular nutrients. These experiments further demonstrate that several motility measuring techniques should be used in parallel to distinguish the various components of cell movement, to exclude aspecific effects, and to supplement the shortcomings of each individual technique. Such procedure could clarify the various discrepancies that have been reported so far and should lead to a better understanding of the regulation of human sperm motility.

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