PREVALENSI DAN KELIMPAHAN PENYAKIT KARANG DI PERAIRAN DESA BUTON KABUPATEN MOROWALI SULAWESI TENGAH

Penyakit karang sekarang ini sudah menjadi perhatian utama para peneliti karang. Banyak kasus dilaporkan penyakit karang menjadi penyumbang terbesar kematian karang di sebuah perairan. Penelitian ini bertujuan untuk mengetahui prevalensi dan kelimpahan penyakit karang di Perairan Desa Buton, Kabupaten Morowali, Sulawesi Tengah. Metode pengambilan data prevalensi dan kelimpahan penyakit karang menggunkan belt transect (transek sabuk) dengan luas 180m2 yang ditarik sejajar garis pantai pada 3 (tiga) titik stasiun. Hasil penelitian menunjukan ditemukan sebanyak 5 (lima) jenis penyakit karang (White Band Disease, Brown Band Disease, Ulcerative White Spot, Bleaching, dan Black Band Disease dan 3 (tiga) jenis gangguan kesehatan karang (Fish bites, Crown-of-Thorn-Starfish (COTS), dan Tube formers). Secara umum total prevalensi penyakit karang di lokasi penelitian sebesar 78,7% (52,3% penyakit karang dan 26,4% berupa gangguan kesehatan karang) dengan kasus tertinggi terdapat di stasiun 1 (satu). lebih lanjut kelimpahan rata-rata penyakit karang sebesar 0,65 koloni/m2. Kelimpahan penyakit karang tertinggi ditemukan pada stasiun I yaitu sebesar 0,32 koloni/m2.Kata Kunci: Penyakit Karang, Prevalensi, Kelimpahan, Perairan Desa Buton

LAJU INFEKSI PENYAKIT WHITE PLAGUE DAN WHITE BAND DISEASE DI PERAIRAN PULAU TIKUS, KOTA BENGKULU

Penyakit karang adalah gangguan terhadap kesehatan karang yang menyebabkan gangguan secara fisiologis bagi biota karang. Penyakit karang dapat memberikan dampak buruk terhadap proses pertumbuhan karang hingga dapat menyebabkan kematian karang disuatu perairan. Dampak dari penyakit karang terhadap ekosistem terumbu karang sangat besar, selain mengakibatkan kematian karang dalam skala yang luas, penyakit karang juga berdampak pada berkurangnya tingkat keanekaragaman sumberdaya alam. Penelitian ini bertujuan untuk mengetahui dan menganalisis laju infeksi penyakit White Plague dan White Band Disease di perairan Pulau Tikus, Kota Bengkulu. Penelitian ini dilaksanakan pada bulan September-Oktober 2019 di Perairan Pulau Tikus, Kota Bengkulu. Metode yang digunakan dalam penelitian ini adalah metode survei, pengamatan dan pengukuran langsung di Lapangan. Hasil Penelitian ini didapatkan bentuk pertumbuhan yang terinfeksi penyakit White Plague di Perairan Pulau Tikus Kota Bengkulu adalah bentuk pertumbuhan karang Massive dan penyakit White Band Disease pada karang Acropora. Metode yang digunakan dalam penelitian ini adalah metode survei, pengamatan dan pengukuran langsung di lapangan.Laju infeksi penyakit White Plague di perairan Pulau Tikus Kota Bengkulu adalah 0,009 - 0,023 cm/hari. Laju infeksi penyakit White Plague mengalami kenaikan setiap minggu dengan rata rata laju infeksi 0,015 cm/hari dan nilai laju infeksi penyakit White Band Disease yang terdapat pada karang Acropora di Perairan Pulau Tikus, Bengkulu yaitu 0,013 cm/hari – 0,030 cm/hari. Rata – rata yang diperoleh yaitu 0,024 cm/hari. Nilai laju infeksi karang menandakan bahwa sebagian besar kerusakan terumbu karang diakibatkan oleh penyakit karang.THE RATE OF WHITE PLAGUE AND WHITE BAND DISEASE IN TIKUS ISLAND, BENGKULU CITY. Coral disease is a disorder of coral health that causes physiological disruption to coral biota. The coral disease can adversely affect the coral growth process until it can lead to coral death in a waterway. The impact of coral disease on coral ecosystems is very large. In addition to resulting in coral death on a wide scale, a coral disease also affects natural resources' reduced diversity. This research aimed to determine and analyze the White Plague and White Band Disease's infection rate in Tikus Island, Bengkulu City. The research was conducted in September-October 2019, located in Tikus Island Water, Bengkulu City. The methods used in this study are direct survey, observation, and measurement methods in the Field. This study obtained a form of growth infected with White Plague disease in the Waters of Tikus Island Bengkulu City. The infection rate of White Plague Disease in Tikus Island was 0.009-0.023 cm/day. The quality of the condition of White Plague disease increased every week with an average infection rate of 0.015 cm/day, and the rate of infection rate of White Band Disease found in Acropora coral in the Waters of Tikus Island, Bengkulu which was 0.013 cm/day – 0.030 cm/day. The average obtained 0.024 cm/day. The coral infection rate indicates that most of the damage to coral reefs was caused by coral disease.

Comparative analysis of bacterial communities associated with healthy and diseased corals in the Indonesian sea

Coral reef ecosystems are impacted by climate change and human activities, such as increasing coastal development, overfishing, sewage and other pollutant discharge, and consequent eutrophication, which triggers increasing incidents of diseases and deterioration of corals worldwide. In this study, bacterial communities associated with four species of corals: Acropora aspera, Acropora formosa, Cyphastrea sp., and Isopora sp. in the healthy and disease stages with different diseases were compared using tagged 16S rRNA sequencing. In total, 59 bacterial phyla, 190 orders, and 307 genera were assigned in coral metagenomes where Proteobacteria and Firmicutes were pre-dominated followed by Bacteroidetes together with Actinobacteria, Fusobacteria, and Lentisphaerae as minor taxa. Principal Coordinates Analysis (PCoA) showed separated clustering of bacterial diversity in healthy and infected groups for individual coral species. Fusibacter was found as the major bacterial genus across all corals. The lower number of Fusibacter was found in A. aspera infected with white band disease and Isopora sp. with white plaque disease, but marked increases of Vibrio and Acrobacter, respectively, were observed. This was in contrast to A. formosa infected by a black band and Cyphastrea sp. infected by yellow blotch diseases which showed an increasing abundance of Fusibacter but a decrease in WH1-8 bacteria. Overall, infection was shown to result in disturbance in the complexity and structure of the associated bacterial microbiomes which can be relevant to the pathogenicity of the microbes associated with infected corals.

KELIMPAHAN DAN PREVALENSI PENYAKIT KARANG DI PERAIRAN LANGARA, KONAWA KEPULAUAN, SULAWESI TENGGARA

Penyakit karang adalah gangguan terhadap kesehatan karang yang menyebabkan hilang atau rusaknya jaringan karang. Biota karang yang terinfeksi penyakit telah diidentifikasikan sebagai salah satu faktor utama yang memperburuk tutupan terumbu karang secara global. Tujuan dari penelitian ini untuk mengetahui kelimpahan dan prevalensi penyakit karang di Perairan Langara, Konawe Kepulauan, Sulawesi Tenggara. Pengambilan data dilakukan di bulan April 2018 pada tiga stasiun penelitian. Metode pengambilan data kelimpahan dan prevalensi penyakit karang menggunakan metode belt transect (transek sabuk) dengan luas area transek 350 m2, yang ditarik sejajar garis pantai. Hasil penelitian menyebutkan bahwa penyakit karang yang ditemukan di lokasi penelitian sebanyak 8 jenis [Black Band Disease (BBD), Dark Spots Disease (DSD), Ulcerative White Spots (UWS), White Band Disease (WBD), Explained Growth Anomalies (EGA), White Syndrom (WS), Bleacing (BL) dan Yellow Band Disease (YBD)]. Kelimpahan dan prevalensi penyakit karang yang tertinggi terdapat di stasiun II, dimana nilai kelimpahan yaitu 0.063 koloni/m2 dan nilai prevalensi yaitu 92.187%. Jenis penyakit karang yang mendominasi di lokasi penelitian secara berturut-turut adalah UWS, BBD dan WS.Kata Kunci: Kelimpahan; Penyakit Karang; Perairan Langara; Prevalensi

KARAKTERISTIK PENYAKIT WHITE BAND DISEASE DAN WHITE SYNDROME SECARA VISUAL DAN HISTOLOGI PADA KARANG Acropora sp. DARI PULAU GILI LABAK SUMENEP MADURA

ABSTRAKPenyakit white band disease dan white syndrome yang menyerang karang Acropora sp. di Pulau Gili Labak merupakan salah satu masalah yang perlu diperhatikan, sebab terjadinya kematian terumbu karang yang disebabkan oleh penyakit karang bukan hanya akan berpengaruh pada fungsi ekologis terumbu karang namun juga akan mempengaruhi fungsi ekonomis. Tujuan penelitian ini adalah untuk mendeteksi penyakit karang dengan metode histologi dengan studi kasus di Pulau Gili Labak, Poteran, Sumenep-Madura. Pengambilan sampel penelitian ini dilaksanakan pada bulan April 2017 di perairan Pulau Gili Labak Kabupaten Sumenep. Penelitian deteksi penyakit pada jaringan karang dengan metode histologi dilakukan pada Laboratorium Histologi Fakultas Kedokteran Universitas Brawijaya Malang. Analisa data dilakukan dengan metode analisa deskriptif. Ditemukan jaringan karang Acropora sp. yang terserang penyakit white band disease dan white syndrome yang terjadi banyak perbedaan antara jaringan karang yang sehat dengan yang sakit. Umumnya pada jaringan karang yang sehat terlihat susunan sel pada jaringan karang terlihat masih baik dan utuh, sedangkan pada jaringan karang yang sakit menunjukan bahwa jaringan mengalami degradasi disebabkan oleh jaringan yang lisis dan nekrosis, jaringan sakit terlihat hilang dan mulai hancur. Penyakit white band disease dicirikan dengan adanya jaringan yang mengelupas dari skeleton karang, sedangkan penyakit white syndrome hilangnya jaringan dimulai pada epidermis terlebih dahulu lalu merambat kedalam skeletonnya. ABSTRACTWhite band disease and white syndrome attacking Acropora sp. on the island of Gili Labak is one of the issues that need to be considered, because the loss of coral caused by disease will not only affect the ecological function of coral reefs but will also affect the economic function. The sampling of this research was conducted in April 2017 in the waters of Gili Labak Island of Sumenep Regency. Research on detection of coral tissue disease by histology method was done at Histology Laboratory Faculty of Medicine Universitas Brawijaya Malang. Data analysis was done by descriptive analysis method. Acropora sp. who are affected by white band disease and white syndrome showed the difference between healthy and affected coral tissues. Generally, on healthy coral tissue, the structure of the cells in coral tissue looks good and intact, whereas in diseased coral tissue show tissue degradation caused by lysis tissue and necrosis, tissue is lost and starts to disintegrate. The white band disease is characterized by the presence of peeling tissue from coral skeleton. While white syndrome disease loss of tissue begins in the epidermis first then propagate into their skeleton.

Bleaching causes loss of disease resistance within the threatened coral species Acropora cervicornis

Determining the adaptive potential of foundation species, such as reef-building corals, is urgent as the oceans warm and coral populations decline. Theory predicts that corals may adapt to climate change via selection on standing genetic variation. Yet, corals face not only rising temperatures but also novel diseases. We studied the interaction between two major stressors affecting colonies of the threatened coral, Acropora cervicornis: white-band disease and high water temperature. We determined that 27% of A. cervicornis were disease resistant prior to a thermal anomaly. However, disease resistance was largely lost during a bleaching event because of more compromised coral hosts or increased pathogenic dose/virulence. There was no tradeoff between disease resistance and temperature tolerance; disease susceptibility was independent of Symbiodinium strain. The present study shows that susceptibility to temperature stress creates an increased risk in disease-associated mortality, and only rare genets may maintain or gain infectious disease resistance under high temperature. We conclude that A. cervicornis populations in the lower Florida Keys harbor few existing genotypes that are resistant to both warming and disease.

Zooplankton as a potential vector for white band disease transmission in the endangered coral,Acropora cervicornis

Coral diseases are a leading factor contributing to the global decline of coral reefs, and yet mechanisms of disease transmission remain poorly understood. This study tested whether zooplankton can act as a vector for white band disease (WBD) inAcropora cervicornis. Natural zooplankton communities were collected from a coral reef in Bocas del Toro, Panama. Half of the zooplankton were treated with antibiotics for 24 h after which the antibiotic-treated and non-antibiotic-treated zooplankton were incubated with either seawater or tissue homogenates from corals exhibiting WBD-like symptoms. A total of 15 of the 30 asymptomaticA. cervicorniscolonies exposed to zooplankton incubated in disease homogenate in tank-based experiments showed signs of WBD, regardless of prior antibiotic incubation. These results indicate that in our experimental conditions zooplankton were a vector for coral disease after exposure to disease-causing pathogens. Given the importance of heterotrophy on zooplankton to coral nutrition, this potential mode of disease transmission warrants further investigation.