Mayetiola destructor (Diptera: Cecidmyiidae) host preference and survival on small grains with respect to leaf reflectance and phytohormone concentrations

The Hessian fly Mayetiola destructor (Diptera: Cecidmyiidae) is a major pest of wheat, globally. We conducted a series of laboratory choice and no-choice assays to quantify Hessian fly host preference for barley (cv. Champion), oat (cv. Cayuse), susceptible (cv. Alturas), and resistant (cv. Hollis) wheat. In addition, larval survivorship and adult emergence were compared among the evaluated host plants. We then examined whether insect preference for a host can be explained by differences in plant spectral reflectance. Further, larval survivorship and adult emergence were compared among host plants in relation to phytohormone concentrations. Hessian flies laid more eggs on wheat compared to either oat or barley. Spectral reflectance measurements of leaves were similar between susceptible and resistant wheat cultivars but different from those of barley and oat. Our results suggested that higher reflectance in the near-infrared range and lower reflectance in the visible range may be used by females for host selection. Hessian fly larvae were unable to develop into the pupal stage on resistant wheat and oat. No significant difference in larval survivorship was detected between the susceptible wheat and barley. However, adult emergence was significantly higher on barley than the susceptible wheat. Phytohormonal evaluations revealed that salicylic acid (SA) may be an important contributor to plant defense response to larval feeding as relatively higher concentrations of SA were present in oat and resistant wheat. While resistance in the resistant wheat is achieved only through antibiosis, both antibiosis and antixenosis were in effect rendering oat as a non-host for Hessian flies.

Detection of Wolbachia Infections in Natural and Laboratory Populations of the Moroccan Hessian Fly, Mayetiola destructor (Say)

Mayetiola destructor (Hessian fly) is a destructive pest of wheat in several parts of the world. Here, we investigated the presence of reproductive symbionts and the effect of the geographical location on the bacterial community associated to adult Hessian flies derived from four major wheat producing areas in Morocco. Using specific 16S rDNA PCR assay, Wolbachia infection was observed in 3% of the natural populations and 10% of the laboratory population. High throughput sequencing of V3-V4 region of the bacterial 16S rRNA gene revealed that the microbiota of adult Hessian flies was significantly influenced by their native regions. A total of 6 phyla, 10 classes and 79 genera were obtained from all the samples. Confirming the screening results, Wolbachia was identified as well in the natural Hessian flies. Phylogenetic analysis using the sequences obtained in this study indicated that there is one Wolbachia strain belonging to supergroup A. To our knowledge, this is the first report of Wolbachia in Hessian fly populations. The observed low abundance of Wolbachia most likely does not indicate induction of reproductive incompatibility. Yet, this infection may give a new insight into the use of Wolbachia for the fight against Hessian fly populations.

Parasitism of Mayetiola destructor (Diptera: Cecidomyiidae) by Platygaster hiemalis (Hymenoptera: Platygasteridae) on Hessian Fly-Resistant Wheats2

Laboratory studies were conducted to determine the effect of three wheat cultivars, ‘Knox 62,’ ‘Caldwell,’ and ‘Monon,’ on the Hessian fly; Mayetiola destructor (Say), and its parasitoid, Platygaster hiemalis Forbes. The number of Hessian flies that survived from the egg to the “flaxseed” stage, the number of Hessian flies parasitized by P. hiemalis, and the average number of parasitoids produced within each parasitized puparium were used to determine the effect of the cultivar on the Hessian fly and indirectly on the parasitoid. The fewest surviving Hessian flies, and the least level of parasitism were found on Knox 62. Cultivars which had high antibiosis against the Hessian fly also reduced the effectiveness of this parasitoid. Furthermore, resistance and parasitism complemented each other in reducing the numbers of Hessian flies.