Comparative prevalence and diversity of Campylobacter jejuni strains in water and human beings over a 1-year period in southwestern Alberta, Canada

Campylobacter jejuni was isolated from diarrheic people, river water (Oldman River watershed), wastewater, and drinking water over a 1-year period in southwestern Alberta (2008-2009). High rates of campylobacteriosis were observed during the study period (≥115 cases/100K). Infections occurred throughout the year, with a peak in late summer and early autumn. The majority of infections occurred in people living in Lethbridge. Campylobacter jejuni was not isolated from municipal drinking water. In contrast, the bacterium was isolated from untreated and treated wastewater, and river water (all sites). There were no correlations between C. jejuni recovery/detection from water and river flow rates, water turbidity, or fecal coliforms. Campylobacter jejuni recovery from water did not correspond with the peak periods of campylobacteriosis. The bacterium was most commonly isolated downstream of wastewater outfalls; waterfowl congregated at these sites, particularly during the winter months. A comparison of subtypes from people and water revealed that the vast majority of subtypes in water did not correspond to subtypes recovered from diarrheic people, and were linked to waterfowl and other non-human animal sources. We conclude that water-borne C. jejuni did not contribute significantly to the high rates of campylobacteriosis observed in people during the study period.

“Having Walked Alongside You”: A Conversational Exchange on Territory and Sound in Motion

As two artist-scholars engaged in research-creation, our goal with this project was to enact a performance/discussion regarding settler-colonialism, sound, performance, and our relationships to land, body and time. During the initial “lockdown phase” of the COVID-19 pandemic in March 2020, we carried out a mediated conversation via WhatsApp voice memos, hiking towards each other along and across the Oldman River, then retracing the other’s path back to our respective points of origin. This collaborative project aims to decolonize the academic paper through a process which combines textual analysis, experiential learning, improvisational performance, and activated writing to ask questions of the relationships between sound, land, and colonial institutions. How does movement through space, and hearing the land, affect our experience of discussing texts? How is discussion informed by, say, the participants being separated (or connected) by a river? What richness exists in oral/aural exchanges that are lost in the textualizing process? What does it mean to move through occupied Blackfoot territory while discussing decolonialism? The structure of this conversational exchange unfolds in loops rather than in the linear standard of academic writing. This “essay” was originally devised as an audiovisual text, but during further revisions, we continued our experimentations with form. The result has taken shape in dual outputs of both sound and text, each form containing affective and sensorial elements not found in the other, creating parallel yet distinct “texts.” While this is an imperfect strategy for those who experience sight/hearing related disabilities, we also recognize that some sensorial experiences are untranslatable into the language of the other senses. We hope that we have encoded each experience of sound and text with enough richness for them to be enjoyed individually, and we invite those who can, to experience how the two forms play off of each other. To this effect, the form of this text focuses on the relationship between the content of our conversation and how it is presented, and between our conversation and the writers, artists and movements we have referenced. We also hope to emphasize our relationality with the land we walked through, the creators of the sounds we listened to, between us, the co-creators of this “text,” as well as the relationship we create with you, the listener/reader experiencing it. As artists/writers/curators, it is a challenge to find alternative textual formats that appropriately reflect artistic research-creation methodologies while also satisfying the demands of academic knowledge dissemination. This collaboration explores the possibility for academic conversations to escape the confines of learning institutions into a space of praxis and embodied experience in motion.

Prevalence and diversity of waterborne Arcobacter butzleri in southwestern Alberta, Canada

Arcobacter butzleri is a potential enteric pathogen to human beings, but its reservoirs and modes of transmission are largely unverified. Microbiological and molecular detection and subtyping techniques can facilitate surveillance of A. butzleri in hosts and environmental reservoirs. We isolated A. butzleri from 173 surface water samples (25.6%) and 81 treated wastewater samples (77.9%) collected in southwestern Alberta over a 1-year period. Arcobacter butzleri isolates (n = 500) were genotyped and compared to determine diversity of A. butzleri in southwestern Alberta. Culture methods affected the frequency of detection and genotype diversity of A. butzleri, and isolation comprehensiveness was different for surface waters and treated wastewaters. Detection of A. butzleri in the Oldman River Watershed corresponded with season, river flow rates, and fecal coliform densities. Arcobacter butzleri was detected most frequently in treated wastewater, in the Oldman River downstream from treated wastewater outfalls, and in tributaries near areas of intensive confined feeding operations. All sample sources possessed high genotype diversity, and A. butzleri isolates from treated wastewaters were genetically similar to isolates from the Oldman River downriver from treated wastewater outfall sites. In southwestern Alberta, municipal and agricultural activities contribute to the density and genotype diversity of A. butzleri in surface waters.

Improving Allocative Efficiency of Scarce Water in Southern Alberta

Using a positive mathematical programming (PMP) model with improved ‘wide-scope’ calibration, this study demonstrates how allocative efficiency of scarce water could be improved in the Bow- and Oldman River Sub Basins (BRSB and ORSB) of Southern Alberta, where 12 irrigation districts, two cities and three major industrial/commercial users withdraw bulk of the surface water for irrigation, municipal, industrial and commercial needs. Earlier studies ironically neglected the larger ORSB even though it is subject to the same water licensing and regulation policies as the BRSB. The inclusion of nine irrigation districts and non-irrigation users of ORSB enables this model to estimate allocative efficiency gains in a more comprehensive manner than before. Results indicate that ORSB has a relatively less elastic water demand curve primarily due to its more reliance on irrigation and less water saving/supply options. It is also less responsive to allocations with alternative policies as reflected in net returns, land use and cropping pattern changes due to its less elastic water demand.

Elevated mercury levels in biota along an agricultural land use gradient in the Oldman River basin, Alberta

This study examines relationships between anthropogenic influence and mercury concentrations in biota along an elevational river gradient with intensifying agricultural and urban land use in the Oldman River basin, Alberta, Canada. We use nitrogen stable isotope signatures (δ15N) indicative of anthropogenic sources of N to indicate the extent of land use influence on the river ecosystem. δ15N values in biota increased by 4.2‰ along the river gradient, consistent with increasing nitrogen sources from sewage and manure. Mercury concentrations in longnose dace ( Rhinichthys cataractae ), suckers ( Catostomus catostomus , Catostomus commersonii ), and net-spinning caddisfly larvae, the most abundant macroinvertebrates, all increased downstream; dace ranged from 0.023 ppm total mercury below the Oldman reservoir to 0.10 ppm total mercury downstream of Lethbridge. Dace consumed mostly insect larvae, and no increase in trophic position (as estimated by δ15N) was observed along the gradient. Fish directly exposed to agricultural and urban effluents had significantly lower mercury levels, or showed no difference, relative to reference sites, which suggests that these effluents play no significant role in elevating mercury levels in river food webs.

Feminization of Longnose Dace (Rhinichthys cataractae) in the Oldman River, Alberta, (Canada) Provides Evidence of Widespread Endocrine Disruption in an Agricultural Basin

We sampled an abundant, native minnow (Longnose dace—Rhinichthys cataractae) throughout the Oldman River, Alberta, to determine physiological responses and possible population level consequences from exposure to compounds with hormone-like activity. Sex ratios varied between sites, were female-biased, and ranged from just over 50% to almost 90%. Histological examination of gonads revealed that at the sites with >60% females in the adult population, there was up to 38% occurrence of intersex gonads in fish identified through visual examination of the gonads as male. In the majority of intersex gonad cases, there was a large proportion (approx., 50%) of oocytes within the testicular tissue. In male dace, vitellogenin mRNA expression generally increased with distance downstream. We analyzed river water for 28 endocrine disrupting compounds from eight functional classes, most with confirmed estrogen-like activity, including synthetic estrogens and hormone therapy drugs characteristic of municipal wastewater effluent, plus natural hormones and veterinary pharmaceuticals characteristic of livestock production. The spatial correlation between detected chemical residues and effects to dace physiology indicate that multiple land uses have a cumulative impact on dace in the Oldman River and effects range from altered gene regulation to severely female-biased sex ratios.