fluorescence label
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2020 ◽  
Vol 130 ◽  
pp. 106345
Author(s):  
Regina Sisika A. Sonthanasamy ◽  
Azwan Mat Lazim ◽  
Siti Nur Syazni Mohd Zuki ◽  
Doris Huai Xia Quay ◽  
Ling Ling Tan

2020 ◽  
Vol 32 (7) ◽  
pp. 1799-1805
Author(s):  
Sheikha A. Alkhursani ◽  
Mohamed Mohamady Ghobashy ◽  
Mohamed Madani

Fluorescence label preparation, being the core of sensing and imaging, is the most interesting aspect of label technology. Using the gamma irradiation technique, a facial method is proposed to prepare organostarch consisting of polyaniline and starch. Polyaniline was introduced into starch molecules to form an inclusion complex between V-type starch and aniline monomer. The inclusion complex thus formed consisted of starch-aniline crosslink caused by gamma irradiation through organostarch crosslinks. Thus, organostarch develops fluorescence property at 470 nm possibly through the interaction of aniline and starch, which are both fluorophores. A comparative analysis of variations is performed in common fluorescent labels of starch and organostarch based on their physico-chemical properties. X-ray diffraction (XRD) and Fourier transform infrared (FTIR) spectrometry were utilized to confirm the inclusion of polyaniline into starch molecules. Furthermore, using a fluorescence microscope, the positive implementation of fluorescent organostarch was verified. Fluorescent organostarch can be synthesized through this simple method and can be widely used for developing biomarkers and biosensors in food and biomedical industries. Organostarch produces florescence under mild conditions even without complicated preparations, such as additives for labelling with dye fluorescence. The intensity of fluorescence of organostarch was 17,000 times that of natural starch.


Author(s):  
George J. Tserevelakis ◽  
Kostas Mavrakis ◽  
Danai Pantazopoulou ◽  
Eleni Karamouzi ◽  
Stella Avtzi ◽  
...  

Polymers ◽  
2019 ◽  
Vol 11 (7) ◽  
pp. 1196 ◽  
Author(s):  
Yuriko Matsumura ◽  
Kaoru Iwai

In order to clarify the local environment during interpolymer complex formation between poly(carboxylic acids) and poly(acrylamide derivatives) with different N-substitutions, a fluorescence label technique was used. 3-(2-propenyl)-9-(4-N,N-dimethylaminophenyl) phenanthrene (VDP) was used as an intramolecular fluorescence probe. All polymers were synthesized by free radical polymerization. Interpolymer complexation was monitored by charge transfer emission from the VDP unit. Both of the poly(carboxylic acids) formed interpolymer complexes with poly(N,N-dimethylacrylamide) (polyDMAM). The micro-environments around the VDP unit in the acidic pH region for the poly(methacrylic acid) (polyMAAc) and polyDMAM mixed systems were more hydrophobic than those of the poly(acrylic acid) (polyAAc) and polyDMAM mixed systems, as the α-methyl group of the MAAc unit contributed to hydrophobicity around the polymer chain during hydrogen bond formation. This suggests that, when the poly(carboxylic acids) and poly(acrylamide derivatives) were mixed, with a subsequent decrease in the solution pH, a hydrogen bond was partially formed, following which the hydrophobicity of the micro-environment around the polymer chains was changed, resulting in the formation of interpolymer complexes. Moreover, the electron-donating ability of the carbonyl group in the poly(acrylamide derivatives) had an effect on complexation with poly(carboxylic acids).


2016 ◽  
Vol 211 ◽  
pp. 611-618 ◽  
Author(s):  
Mathilde Lepoitevin ◽  
Mikhael Bechelany ◽  
Emmanuel Balanzat ◽  
Jean-Marc Janot ◽  
Sebastien Balme

2016 ◽  
Vol 138 (28) ◽  
pp. 8976-8981 ◽  
Author(s):  
Yingjie Zhao ◽  
Richard H. M. Bernitzky ◽  
Max J. Kory ◽  
Gregor Hofer ◽  
Johan Hofkens ◽  
...  

2011 ◽  
Vol 46 (12) ◽  
pp. 2296-2303 ◽  
Author(s):  
Tong Liu ◽  
Yu Wang ◽  
Hanjiao Qin ◽  
Xue Bai ◽  
Biao Dong ◽  
...  

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