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2021 ◽  
Vol 2021 (5) ◽  
pp. pdb.prot101824
Author(s):  
Michael R. Green ◽  
Joseph Sambrook
Keyword(s):  

2019 ◽  
Vol 74 (11) ◽  
pp. 3199-3204 ◽  
Author(s):  
A Baraniak ◽  
M Machulska ◽  
D Żabicka ◽  
E Literacka ◽  
R Izdebski ◽  
...  

AbstractObjectivesIn 2015 and 2016 Poland recorded rapid proliferation of New Delhi MBL (NDM)-producing Enterobacterales, with at least 470 and 1780 cases, respectively. We addressed the roles of the Klebsiella pneumoniae ST11 NDM-1 outbreak genotype, already spreading in 2012–14, and of newly imported organisms in this increase.MethodsThe study included 2136 NDM-positive isolates identified between April 2015 and December 2016, following transfer of patients with K. pneumoniae ST147 NDM-1 from Tunisia to Warsaw in March 2015. The isolates were screened by PCR mapping for variants of blaNDM-carrying Tn125-like elements. Selected isolates were typed by PFGE and MLST. NDM-encoding plasmids were analysed by nuclease S1/hybridization, transfer assays, PCR-based replicon typing and PCR mapping.ResultsThe organisms were mainly K. pneumoniae containing the Tn125A variant of the ST11 epidemic lineage (n = 2094; ∼98%). Their representatives were of the outbreak pulsotype and ST11, and produced NDM-1, encoded by specific IncFII (pKPX-1/pB-3002cz)-like plasmids. The isolates were recovered in 145 healthcare centres in 13/16 administrative regions, predominantly the Warsaw area. The ‘Tunisian’ genotype K. pneumoniae ST147 NDM-1 Tn125F comprised 18 isolates (0.8%) from eight institutions. The remaining 24 isolates, mostly K. pneumoniae and Escherichia coli of diverse STs, produced NDM-1 or NDM-5 specified by various Tn125 derivatives and plasmids.ConclusionsThe K. pneumoniae ST11 NDM-1 outbreak has dramatically expanded in Poland since 2012, which may bring about a countrywide endemic situation in the near future. In addition, the so-far limited K. pneumoniae ST147 NDM-1 outbreak plus multiple NDM imports from different countries were observed in 2015–16.


2019 ◽  
Vol 76 (1) ◽  
pp. e76 ◽  
Author(s):  
Arivazhagan Rajendran ◽  
Narumi Shigi ◽  
Jun Sumaoka ◽  
Makoto Komiyama

Author(s):  
Arivazhagan Rajendran ◽  
Narumi Shigi ◽  
Jun Sumaoka ◽  
Makoto Komiyama

2015 ◽  
Vol 20 (2) ◽  
pp. 413-419 ◽  
Author(s):  
Yunlei Zhou ◽  
Mo Wang ◽  
Zhiqing Yang ◽  
Linan Lu ◽  
Huanshun Yin ◽  
...  

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