Mapping RNA with Nuclease S1

2021 ◽  
Vol 2021 (5) ◽  
pp. pdb.prot101824
Author(s):  
Michael R. Green ◽  
Joseph Sambrook
Keyword(s):  
Nuclease S1

Artificial Restriction DNA Cutter Using Nuclease S1 for Site‐Selective Scission of Genomic DNA

2019 ◽  
pp. e72 ◽  
Author(s):  
Arivazhagan Rajendran ◽  
Narumi Shigi ◽  
Jun Sumaoka ◽  
Makoto Komiyama
Keyword(s):  
Genomic Dna ◽  
Nuclease S1 ◽  
Artificial Restriction ◽  
Site Selective

Affinity Isolation of Defined Genomic Fragments Cleaved by Nuclease S1-based Artificial Restriction DNA Cutter

2019 ◽  
Vol 76 (1) ◽  
pp. e76 ◽  
Author(s):  
Arivazhagan Rajendran ◽  
Narumi Shigi ◽  
Jun Sumaoka ◽  
Makoto Komiyama
Keyword(s):  
Nuclease S1 ◽  
Affinity Isolation ◽  
Artificial Restriction

scholarly journals Specific cleavage of the terminal protein from the adenovirus 5 DNA under the condition of single-strand scission by nuclease S1

FEBS Letters ◽  
1979 ◽  
Vol 107 (2) ◽  
pp. 355-358 ◽  
Author(s):  
Hiroyoshi Ariga ◽  
Hiroto Shimojo ◽  
So Hidaka ◽  
Kin-ichiro Miura
Keyword(s):  
Single Strand ◽  
Terminal Protein ◽  
Strand Scission ◽  
Nuclease S1 ◽  
Adenovirus 5

Acridine-bearing PNA for Efficient Protection of Designated Site of DNA from Nuclease S1

2003 ◽  
Vol 32 (8) ◽  
pp. 714-715
Author(s):  
Binzhi Ren ◽  
Sheng Ye ◽  
Xingguo Liang ◽  
Makoto Komiyama
Keyword(s):  
Nuclease S1 ◽  
Efficient Protection

scholarly journals Testosterone induces the expression of the uteroglobin gene in rabbit epididymis

1988 ◽  
Vol 250 (3) ◽  
pp. 647-651 ◽  
Author(s):  
M S López de Haro ◽  
L Alvarez ◽  
A Nieto
Keyword(s):  
Northern Blot ◽  
Distal Part ◽  
Hormonal Treatment ◽  
Middle Region ◽  
Beta Tubulin ◽  
S1 Mapping ◽  
Nuclease S1 ◽  
Tubulin Mrna

Uteroglobin was characterized in the rabbit epididymis by radioimmunoassay and electrophoretic determinations, as well as by analysis of its mRNA by means of ‘Northern blot’ and nuclease-S1 mapping. Treatment of sexually immature rabbits with testosterone during 5 days increased up to 8-fold the concentrations of both uteroglobin and its mRNA in the epididymis. The amounts of beta-tubulin mRNA, measured as reference, remained unchanged after the hormonal treatment. The synthesis of uteroglobin occurred mainly in the middle region of the epididymis, progressively decreasing toward the distal part of the organ. Uteroglobin was not detected in the testis by radioimmunoassay. The results are discussed in relation to a possible role of uteroglobin in the reproductive functions.

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