SOLID PHASE EXTRACTION BASED ON Mn3O4-TIO2 NANOCOMPOSITE FOR THE DETERMINATION OF MOLYBDENUM IN WATER SAMPLES

The Mn3O4-TiO2 nanocomposite was applied as a new solid phase adsorbent for preconcentration of Molybdenum before determination by an UV-Vis spectrophotometer. The solid-phase extraction conditions as pH, the mass of sorbent, contact time, stirring speed, and the elution condition were investigated. Under optimized conditions, the adsorption capacity of adsorbent was 20.69 mg/g, and the elution condition was 0.2M NaOH solution. Molybdenum ions were determined based on its catalytic effect on the oxidation of 1-amino-2- naphthol-4-sulfonic acid (ANSA) with H2O2. The linear calibration graph was in the range of 0.2–2.5 µg/L (r2 = 0.995) with a detection limit of 0.0502 µg/L. The relative standard deviation for five measurements of 2 µg/L of Mo (VI) was 3,37%. The method was applied to the determination of Molybdenum in water samples.

Development of novel small peptide ligands for antibody purification

Small peptide ligands which were designed based on the interactions with human immunoglobulin G (IgG) using the molecular simulations, can offer a potential alternative for mAb purification with elution condition at pH 9 and pH 3.