biolistic process
Recently Published Documents


TOTAL DOCUMENTS

13
(FIVE YEARS 0)

H-INDEX

6
(FIVE YEARS 0)

2001 ◽  
Vol 34 (9) ◽  
pp. 1115-1124
Author(s):  
L.A. Ribeiro ◽  
P.D.S.C. Mariani ◽  
J.L. Azevedo ◽  
E.L. Rech ◽  
G.S. Schmidt ◽  
...  

1998 ◽  
Vol 44 (12) ◽  
pp. 1137-1141 ◽  
Author(s):  
Fernando Gomes Barcellos ◽  
Maria Helena Pelegrinelli Fungaro ◽  
Márcia Cristina Furlaneto ◽  
Bernard Lejeune ◽  
Aline A Pizzirani-Kleiner ◽  
...  

Mitotically unstable Aspergillus nidulans argB+ transformants obtained by the biolistic process were studied in the present work. Hybridization signals from undigested DNA and pulsed-field chromosomal bands of the transformants suggested the introduced plasmid occurred as free concatenated molecules. Fifteen vigorous growth sectors released from the transformants were analysed in order to understand the mechanisms involved in their formation. All sectors showed the integration of exogenous genes into the fungal genome by homologous or heterologous recombinant events.Key words: Aspergillus nidulans, biolistic process, mitotic instability, fungal transformation.


1998 ◽  
Vol 44 (12) ◽  
pp. 1137-1141 ◽  
Author(s):  
Fernando Gomes Barcellos ◽  
Maria Helena Pelegrinelli Fungaro ◽  
Márcia Cristina Furlaneto ◽  
Bernard Lejeune ◽  
Aline A. Pizzirani-Kleiner ◽  
...  

1997 ◽  
Vol 43 (8) ◽  
pp. 718-722
Author(s):  
Maria Helena P. Fungaro ◽  
Regina C. Poli ◽  
Aline A. Pizzirani-Kleiner ◽  
João Lucio Azevedo ◽  
Evelyne Besin ◽  
...  

Twenty transformants obtained by transforming the argB strain of Aspergillus nidulans by the biolistic process were analysed in detail. Eight lost the Arg+ phenotype very frequently and the other 12 were mitotically stable, even after 15 successive subculturing. To analyse the integration events, the mitotically stable transformants were submited to genetic and molecular analyses; 16.7% resulted from the integration of the transforming DNA in tandem with the chromosomal argB locus, 8.3% showed replacement of the argB mutation by wild type allele, 41.7% showed transforming DNA integrated into nonhomologous chromosomal regions at chromosome III, and 33.3% showed transforming DNA integrated into nonhomologous chromosomal regions at chromosomes others than III. Among the mitotically stable transformants, the frequency of integration into nonhomologous sites was higher by the biolistic process than that reported by the literature for protoplast-mediated transformation.Key words: Aspergillus nidulans, fungal transformation, biolistic gene transfer, gene integration, argB gene.


1997 ◽  
Vol 7 (2) ◽  
pp. 139-143 ◽  
Author(s):  
Priscilla A. Furth

Plant Science ◽  
1995 ◽  
Vol 111 (2) ◽  
pp. 209-218 ◽  
Author(s):  
Christian Godon ◽  
Michel Caboche ◽  
Françoise Daniel-Vedele

Sign in / Sign up

Export Citation Format

Share Document