Gene transfer by biolistic process

1997 ◽  
Vol 7 (2) ◽  
pp. 139-143 ◽  
Author(s):  
Priscilla A. Furth
Keyword(s):  
Gene Transfer ◽  
Biolistic Process

scholarly journals GENETIC TRANSFORMATION OF ROSE (ROSA SPP) USING THE BIOLISTIC PROCESS.

HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 659e-659
Author(s):  
M. S. Strefeler
Keyword(s):  
Gene Transfer ◽  
Genetic Transformation ◽  
Transformation Efficiency ◽  
New Genes ◽  
Improvement Programs ◽  
Foreign Dna ◽  
Cultivar Improvement ◽  
Time Required ◽  
Biolistic Process ◽  
Elite Germplasm

Genetic transformation of cut roses may greatly facilitate cultivar improvement programs by shortening the time required to introduce new genes into elite germplasm. The biolistic process offers a very promising method for the genetic transformation of roses. The biolistic process uses high velocity mircoprojectiles (gold or tungsten) to carry foreign DNA into cells. This process has been shown to be useful for genetic transformation of many organisms. The first step in taking advantage of this process is to optimize the factors which affect transformation efficiency. Several factors that have a significant affect on transformation efficiency were examined in an effort to optimize the biolistic process for gene transfer in roses. The factors examined were type of tissue (leaf segments, petioles, callus, etc), bombardment distance, the number of bombardments, DNA construct and microcarrier velocity. The reporter gene, GUS, was used for determining transformation efficiency in this study. GUS was carried on several plasmid constructs which also contained antibiotic resistance (kanamycin or streptomycin. Efficiency of gene transfer was determined by calculating the number of transiently expressing GUS cells for each combination of factors. Results of this study will be discussed and summarized.

scholarly journals A biolistic process for in vitro gene transfer into chicken embryos

2001 ◽  
Vol 34 (9) ◽  
pp. 1115-1124
Author(s):  
L.A. Ribeiro ◽  
P.D.S.C. Mariani ◽  
J.L. Azevedo ◽  
E.L. Rech ◽  
G.S. Schmidt ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Chicken Embryos ◽  
Biolistic Process

Phagocytosis of opsonized red blood cells by hepatocytes after gene transfer of chimeric Fc γR III Aα and γcDNA

2001 ◽  
Vol 120 (5) ◽  
pp. A356-A357
Author(s):  
M FURUKAWA ◽  
Y MAGAMI ◽  
D NAKAYAMA ◽  
F MORIYASU ◽  
J PARK ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Red Blood Cells ◽  
Blood Cells

1860: Generation of Fluorescent Sperm by Use of in VIVO Gene Transfer to Hamster Testes

2007 ◽  
Vol 177 (4S) ◽  
pp. 617-618
Author(s):  
Hiraki Kubota ◽  
Kevin Coward ◽  
Olivia Hibbitt ◽  
Nilendran Prathalingam ◽  
William Holt ◽  
...  
Keyword(s):  
Gene Transfer ◽  
In Vivo Gene Transfer

1509: Adeno-Associated Virus-Mediated Human IL-10 Gene Transfer Suppresses the Development of Experimental Autoimmune Orchitis

2005 ◽  
Vol 173 (4S) ◽  
pp. 409-409
Author(s):  
Masami Watanabe ◽  
Atsushi Nagai ◽  
Norihiro Kusumi ◽  
Yasutomo Nasu ◽  
Hiromi Kumon ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Adeno Associated Virus ◽  
Autoimmune Orchitis ◽  
Experimental Autoimmune

1011: Suppression of Renal Cell Carcinoma Lung Metastasis by Adeno-Associated Virus (AAV) Mediated Gene Transfer of a Soluble Receptor of VEGF

2004 ◽  
Vol 171 (4S) ◽  
pp. 267-267
Author(s):  
Ichiro Yoshimura ◽  
Yasunori Mizuguchi ◽  
Akira Miyajima ◽  
Tomohiko Asano ◽  
Hiroaki Mizukami ◽  
...  
Keyword(s):  
Renal Cell Carcinoma ◽  
Gene Transfer ◽  
Cell Carcinoma ◽  
Lung Metastasis ◽  
Renal Cell ◽  
Soluble Receptor ◽  
Adeno Associated Virus
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