methyl labeling
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ChemBioChem ◽  
2020 ◽  
Author(s):  
Inguna Goba ◽  
David Goricanec ◽  
Dominik Schum ◽  
Matthias Hillenbrand ◽  
Andreas Plückthun ◽  
...  

2020 ◽  
Vol 56 (74) ◽  
pp. 10890-10893
Author(s):  
Thomas Schmidt ◽  
G. Marius Clore

Methyl protonation in a deuterated protein background is used to assign DEER-derived multimodal distance distributions by phase-memory relaxation time filtering.


2019 ◽  
Vol 73 (12) ◽  
pp. 687-697 ◽  
Author(s):  
Rustam Ali ◽  
Lindsay D. Clark ◽  
Jacob A. Zahm ◽  
Andrew Lemoff ◽  
Karthik Ramesh ◽  
...  

Abstract Site specific methyl labeling combined with methyl TROSY offers a powerful NMR approach to study structure and dynamics of proteins and protein complexes of high molecular weight. Robust and cost-effective methods have been developed for site specific protein 1H/13C methyl labeling in an otherwise deuterated background in bacteria. However, bacterial systems are not suitable for expression and isotope labeling of many eukaryotic and membrane proteins. The yeast Pichia pastoris (P. pastoris) is a commonly used host for expression of eukaryotic proteins, and site-specific methyl labeling of perdeuterated eukaryotic proteins has recently been achieved with this system. However, the practical utility of methyl labeling and deuteration in P. pastoris is limited by high costs. Here, we describe an improved method for 1H/13C-labeling of the δ-methyl group of isoleucine residues in a perdeuterated background, which reduces the cost by ≥ 50% without compromising the efficiency of isotope enrichment. We have successfully implemented this method to label actin and a G-protein coupled receptor. Our approach will facilitate studies of the structure and dynamics of eukaryotic proteins by NMR spectroscopy.


ChemPhysChem ◽  
2018 ◽  
Vol 19 (19) ◽  
pp. 2457-2460 ◽  
Author(s):  
Maximilian Zinke ◽  
Pascal Fricke ◽  
Sascha Lange ◽  
Sophie Zinn-Justin ◽  
Adam Lange

2018 ◽  
Vol 13 (8) ◽  
pp. 2170-2178 ◽  
Author(s):  
Mariya Lazarova ◽  
Frank Löhr ◽  
Ralf-Bernhardt Rues ◽  
Robin Kleebach ◽  
Volker Dötsch ◽  
...  
Keyword(s):  

2017 ◽  
Vol 112 (3) ◽  
pp. 487a-488a
Author(s):  
Andrew Hinck ◽  
Morkos A. Henen ◽  
Christian Zwieb ◽  
Ravindra Kodali ◽  
Cynthia S. Hinck

2016 ◽  
Vol 65 (2) ◽  
pp. 99-108 ◽  
Author(s):  
Yoan R. Monneau ◽  
Yojiro Ishida ◽  
Paolo Rossi ◽  
Tomohide Saio ◽  
Shiou-Ru Tzeng ◽  
...  

2016 ◽  
Vol 64 (3) ◽  
pp. 267-267
Author(s):  
Lindsay Clark ◽  
Jacob A. Zahm ◽  
Rustam Ali ◽  
Maciej Kukula ◽  
Liangqiao Bian ◽  
...  

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