scholarly journals Tm filtering by 1H-methyl labeling in a deuterated protein for pulsed double electron–electron resonance EPR

2020 ◽  
Vol 56 (74) ◽  
pp. 10890-10893
Author(s):  
Thomas Schmidt ◽  
G. Marius Clore
Keyword(s):  
Relaxation Time ◽  
Electron Resonance ◽  
Methyl Labeling ◽  
Distance Distributions ◽  
Deuterated Protein ◽  
Double Electron ◽  
Phase Memory ◽  
Double Electron Electron Resonance ◽  
Methyl Protonation

Methyl protonation in a deuterated protein background is used to assign DEER-derived multimodal distance distributions by phase-memory relaxation time filtering.

scholarly journals Strategies to identify and suppress crosstalk signals in double electron–electron resonance (DEER) experiments with gadolinium<sup>III</sup> and nitroxide spin-labeled compounds

2020 ◽  
Vol 1 (2) ◽  
pp. 285-299
Author(s):  
Markus Teucher ◽  
Mian Qi ◽  
Ninive Cati ◽  
Henrik Hintz ◽  
Adelheid Godt ◽  
...  
Keyword(s):  
Relaxation Times ◽  
Molecular Complexes ◽  
Spin Labels ◽  
Electron Resonance ◽  
Molecular Rulers ◽  
Distance Distributions ◽  
Double Electron ◽  
Biomolecular Complexes ◽  
Double Electron Electron Resonance ◽  
Intermolecular Distances

Abstract. Double electron–electron resonance (DEER) spectroscopy applied to orthogonally spin-labeled biomolecular complexes simplifies the assignment of intra- and intermolecular distances, thereby increasing the information content per sample. In fact, various spin labels can be addressed independently in DEER experiments due to spectroscopically nonoverlapping central transitions, distinct relaxation times, and/or transition moments; hence, they are referred to as spectroscopically orthogonal. Molecular complexes which are, for example, orthogonally spin-labeled with nitroxide (NO) and gadolinium (Gd) labels give access to three distinct DEER channels that are optimized to selectively probe NO–NO, NO–Gd, and Gd–Gd distances. Nevertheless, it has been previously recognized that crosstalk signals between individual DEER channels can occur, for example, when a Gd–Gd distance appears in a DEER channel optimized to detect NO–Gd distances. This is caused by residual spectral overlap between NO and Gd spins which, therefore, cannot be considered as perfectly orthogonal. Here, we present a systematic study on how to identify and suppress crosstalk signals that can appear in DEER experiments using mixtures of NO–NO, NO–Gd, and Gd–Gd molecular rulers characterized by distinct, nonoverlapping distance distributions. This study will help to correctly assign the distance peaks in homo- and heterocomplexes of biomolecules carrying not perfectly orthogonal spin labels.

Reliable nanometre-range distance distributions from 5-pulse double electron electron resonance

2017 ◽  
Vol 19 (24) ◽  
pp. 15754-15765 ◽  
Author(s):  
Frauke D. Breitgoff ◽  
Yevhen O. Polyhach ◽  
Gunnar Jeschke
Keyword(s):  
Signal Processing ◽  
Electron Resonance ◽  
Experimental Time ◽  
Distance Distributions ◽  
Double Electron ◽  
Double Electron Electron Resonance

The partial excitation artefact in 5-pulse DEER data can be eliminated by experimental time shifting and signal processing.

scholarly journals Double-Arm Lanthanide Tags Deliver Narrow Gd3+ -Gd3+ Distance Distributions in Double Electron-Electron Resonance (DEER) Measurements

2017 ◽  
Vol 23 (48) ◽  
pp. 11694-11702 ◽  
Author(s):  
Adarshi P. Welegedara ◽  
Yin Yang ◽  
Michael D. Lee ◽  
James D. Swarbrick ◽  
Thomas Huber ◽  
...  
Keyword(s):  
Electron Resonance ◽  
Distance Distributions ◽  
Double Electron ◽  
Double Electron Electron Resonance

scholarly journals Overcoming artificial broadening in Gd3+–Gd3+ distance distributions arising from dipolar pseudo-secular terms in DEER experiments

2016 ◽  
Vol 18 (18) ◽  
pp. 12847-12859 ◽  
Author(s):  
Marie Ramirez Cohen ◽  
Veronica Frydman ◽  
Petr Milko ◽  
Mark A. Iron ◽  
Elwy H. Abdelkader ◽  
...  
Keyword(s):  
Electron Resonance ◽  
Distance Distributions ◽  
Double Electron ◽  
Double Electron Electron Resonance ◽  
Secular Terms

Double electron–electron resonance (DEER) is used to probe structure of Gd3+-tagged biomolecules by determining Gd3+–Gd3+ distances.

Direct Observation of Chain Lengths and Conformations in Oligofluorene Distributions from Controlled Polymerization by Double Electron-Electron Resonance

2019 ◽  
Author(s):  
Dennis Bücker ◽  
Annika Sickinger ◽  
Julian D. Ruiz Perez ◽  
Manuel Oestringer ◽  
Stefan Mecking ◽  
...  
Keyword(s):  
Chain Length ◽  
Length Distribution ◽  
Catalyst System ◽  
Chain Conformation ◽  
Controlled Polymerization ◽  
Chain Length Distribution ◽  
Electron Resonance ◽  
Double Electron ◽  
The Individual ◽  
Double Electron Electron Resonance

Synthetic polymers are mixtures of different length chains, and their chain length and chain conformation is often experimentally characterized by ensemble averages. We demonstrate that Double-Electron-Electron-Resonance (DEER) spectroscopy can reveal the chain length distribution, and chain conformation and flexibility of the individual n-mers in oligo-(9,9-dioctylfluorene) from controlled Suzuki-Miyaura Coupling Polymerization (cSMCP). The required spin-labeled chain ends were introduced efficiently via a TEMPO-substituted initiator and chain terminating agent, respectively, with an in situ catalyst system. Individual precise chain length oligomers as reference materials were obtained by a stepwise approach. Chain length distribution, chain conformation and flexibility can also be accessed within poly(fluorene) nanoparticles.

In-Cell Double Electron–Electron Resonance at Nanomolar Protein Concentrations

2021 ◽  
pp. 3679-3684
Author(s):  
Svetlana Kucher ◽  
Christina Elsner ◽  
Mariya Safonova ◽  
Stefano Maffini ◽  
Enrica Bordignon
Keyword(s):  
Electron Resonance ◽  
Double Electron ◽  
Double Electron Electron Resonance
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