Understanding the synergistic effect of physicochemical properties of nanoparticles and their cellular entry pathways

Gaining precise control over the cellular entry pathway of nanomaterials is key in achieving cytosolic delivery, accessing subcellular environments, and regulating toxicity. However, this precise control requires a fundamental understanding of the behavior of nanomaterials at the bio-nano interface. Herein, we report a computational study investigating the synergistic effect of several key physicochemical properties of nanomaterials on their cellular entry pathways. By examining interactions between monolayer-protected nanoparticles and model cell membranes in a three-dimensional parameter space of size, surface charge/pKa, and ligand chemistry, we observed four different types of nanoparticle translocation for cellular entry which are: outer wrapping, free translocation, inner attach, and embedment. Nanoparticle size, surface charge/pKa, and ligand chemistry each play a unique role in determining the outcome of translocation. Specifically, membrane local curvature induced by nanoparticles upon contact is critical for initiating the translocation process. A generalized paradigm is proposed to describe the fundamental mechanisms underlying the bio-nano interface.

Predicting the effect of chain-length mismatch on phase separation in noble metal nanoparticle monolayers with chemically mismatched ligands

Enhanced Monte Carlo sampling can be used to predict the morphology of mixed ligand nanoparticle monolayers, providing a step forward in the design of monolayer protected nanoparticles for biosensing, drug delivery, and photonics.

Toward high value sensing: monolayer-protected metal nanoparticles in multivariable gas and vapor sensors

This review provides analysis of advances in multivariable sensors based on monolayer-protected nanoparticles and several principles of signal transduction that result in building non-resonant and resonant electrical sensors as well as material- and structure-based photonic sensors.