Citizen Science: Society takes centre stage

In the past decade, scientific research that relies on the collaboration of citizens has grown exponentially. Be it for collecting data on bird migrations, noise pollution, or empty houses in a neighborhood, or for analyzing pictures of malignant cells or distant stars, or for transcribing ship logs or translating Egyptian hieroglyphs (all examples of real citizen science projects), there are countless opportunities for society to get involved in the work done by scientists and contribute to the accumulation of scientific knowledge. Also, other levels of engagement are possible beyond collecting or analyzing data: suggesting research topics, designing research methods, interpreting research results, discussing and disseminating findings. This Metode SSJ monograph brings together contributions from several countries and from the perspective of diverse kinds of citizen science. From consultations to understand ways to improve science communication to increasing science and sustainability awareness through games and activities, exploring mental health support networks and analysing instruments for measuring radioactivity after a nuclear disaster. Concomitantly, issues such as levels of participation, the potential for doing citizen science in the social sciences, the impacts of education, or the role of digital applications are discussed.

A Bird's Eye View: More-Than-Human Migrations

With a focus on bird migrations, this essay seeks to understand Derrida’s (2000) concept of hospitality and its corollary, the relationship between guest and host, in the more-than-human world. The essay begins by considering the implications of migratory movement on the more-than-human “hosts” residing in both summer and winter habitats. It then considers how, depending on one’s perspective, migratory bird populations might be considered both guests and hosts simultaneously in/of two locals, and yet also foreigners as they move out, through, and into various territories. I use this three-part paradox to tease out subtle distinctions toward an understanding of hospitality in relation to both humans and more-than-human contexts. This essay also draws on key related concepts from Deleuze and Guattari (1987) and uses Barad’s (2007) diffractive methodology to understand the intersection of hospitality and migration in more-than-human and human landscapes.

Biological Properties and Genetic Characterization of Novel Low Pathogenic H7N3 Avian Influenza Viruses Isolated from Mallard Ducks in the Caspian Region, Dagestan, Russia

Avian influenza viruses (AIVs) are maintained in wild bird reservoirs, particularly in mallard ducks and other waterfowl. Novel evolutionary lineages of AIV that arise through genetic drift or reassortment can spread with wild bird migrations to new regions, infect a wide variety of resident bird species, and spillover to domestic poultry. The vast continental reservoir of AIVs in Eurasia harbors a wide diversity of influenza subtypes, including both highly pathogenic (HP) and low pathogenic (LP) H7 AIV. The Caspian Sea region is positioned at the intersection of major migratory flyways connecting Central Asia, Europe, the Black and Mediterranean Sea regions and Africa and holds a rich wetland and avian ecology. To understand genetic reservoirs present in the Caspian Sea region, we collected 559 cloacal swabs from Anseriformes and other species during the annual autumn migration periods in 2017 and 2018. We isolated two novel H7N3 LPAIV from mallard ducks whose H7 hemagglutinin (HA) gene was phylogenetically related to contemporaneous strains from distant Mongolia, and more closely Georgia and Ukraine, and predated the spread of this H7 LPAIV sublineage into East Asia in 2019. The N3 neuraminidase gene and internal genes were prototypical of AIV widely dispersed in wild bird reservoirs sampled along flyways connected to the Caspian region. The polymerase and nucleoprotein segments clustered with contemporaneous H5 HPAI (clade 2.3.4.4b) isolates, suggesting the wide dispersal of H7 LPAIV and the potential of this subtype for reassortment. These findings highlight the need for deeper surveillance of AIV in wild birds to better understand the extent of infection spread and evolution along spatial and temporal flyways in Eurasia.

THE VIROME OF RHIPICEPHALUS, DERMACENTOR AND HAEMAPHYSALIS TICKS FROM EASTERN ROMANIA INCLUDES NOVEL VIRUSES WITH POTENTIAL RELEVANCE FOR PUBLIC HEALTH

Ticks are involved in the transmission of various pathogens and some tick-borne diseases cause significant problems for the health of humans and livestock. Despite their obvious importance, the composition of viral communities in ticks, and their interactions with pathogens, is poorly understood, particularly in Eastern Europe that constitutes (via bird migrations for example) a major hub for animal-arthropod vectors exchanges. The aim of this study was first to describe the virome of Dermacentor sp., Rhipicephalus sp. and Haemaphysalis sp. ticks collected from poorly investigated regions of Romania (Iasi and Tulcea counties) located at the intersection of various biotopes, countries and routes of migrations. We then focused the study on viruses that could have potential relevance for human and animal health. More than 500 ticks were collected in 2019 from the environment and from small ruminants and analyzed by high-throughput transcriptome sequencing. Among the viral communities infecting Romanian ticks, viruses belonging to the Flaviviridae, Phenuiviridae and Nairoviridae families were identified and full genomes were derivedPhylogenetic analyses placed them in clades where mammalian isolates are found, suggesting that these viruses could constitute novel arboviruses. We also assessed the bacterial microbiome of the collected ticks. The characterization of these microbial communities increases the knowledge of the diversity of viruses in Eastern Europe and provide a basis for further studies on the relationship between ticks and tick-borne viruses.

Characteristics of bird's visible migrations at the Dniester Delta in 1974-1976

The article deals with the results of observations of diurnal bird migrations and counts on a fixed route in the spring and autumn of 1974-1976, in the Dniester Delta. The total number of observation days was 82 (32 in spring and 50 in autumn, respectively). For this period, during observations and counts on the route, 135 bird species from 11 orders were registered, the total number of which exceeded 600 000 individuals. The study of migrations was carried out according to the E. Kumari method (1955). At the observation site, 80 species were counted, including 52 in spring and 60 in autumn. The Jaccard similarity coefficient (Jaccard index) was quite low between seasons and amounted to only 0.59. The frequency of occurrence of the majority of registered species was low. This may have been due to the area of the floodplains themselves, as well as to the weak extent of their anthropogenic transformation observed in the late 70s of the 20th century, which contributed to the bird migrations over the Delta in a wide front. The density of the spring migration flow was maximum in March and averaged 1077.4 ind. (n=32) per a daylight, while the autumn migration flow was 1926.2 ind. (n=50). There were interannual differences in the density of migrations in March. In the spring of 1975, the flight density was 900.3 ind. (n=15), and in 1976 it was almost twice more and amounted to 2094.7 ind. (n=11). There were no interannual differences in the intensity of autumn migrations. The maximum migration density was 2585.2 ind. (n=23) in October. The majority of non-passerine bird species flew in the morning: in spring up to 43.8%, in autumn - 64.1%. The diurnal activity of Passeriformes varied by season: in spring, morning migrations prevailed (56.1%), and in autumn, with the same ratio in the evening (56.1%). The vast majority of flocks (87 - 90%) amounted from 1 to 50 individuals. At altitudes up to 50 m, 37.0% flew, 51-150 m – 24.9% and above 150 m – 38.1% of birds. For a number of species, it was proved that the height of their flights in the general for the season migration sector of directions was higher than in the reverse directions.127 bird species were registered on all routes, of which 106 were registered on a permanent (fixed) route. Maximum species diversity was observed in March – 85, in April − 65, in September − 60, in October − 61 and in November – 40 species. The dynamics of the species composition of birds on a fixed route can be used as an additional characteristic of day transit migrations.

Seasonal Spatial Dynamics of Butterfly Migration

Understanding the seasonal movements of migratory species underpins ecological studies. Nearly 600 butterfly species show migratory behaviour, yet the spatial pattern of these migrations is poorly understood. We developed climatic niche models for 405 migratory butterfly species globally to estimate patterns of seasonal movement and the distribution of seasonal habitat suitability. We discover strong seasonal variation in habitat suitability for most of the migratory butterflies with >75% of pixels showing seasonal change in predicted occupancy for 85% of species. In contrast to bird migrations, the greatest rate of seasonal switching occurs in the tropics. Several species showed extreme range fluctuations, exceeding 10-fold for 53 species (13%) and more than 100-fold for nine species (2%); such species may be at elevated extinction risk. Our results can be used to search for the ecological processes that underpin migration in insects, as well as to design conservation interventions for declining migratory insects.

Fruit and berry plants of forest belts as a factor of species diversity of ornithofauna during the breeding season and autumn migration period

During migration, the availability of food that affects the success of bird movements, the nature and timing of their movements, is critical for many bird species. The relationship between migration routes and the ripening of fruit and berry plants along the route is important. Four types of forest belts were studied: wind-blown maple-ash, latticed maple-linden, dense oak-maple-linden, wind-blown oak-maple-poplar. During the study 43 bird species were identified consuming 9 major fruit and berry plant species: Sambucus nigra, Prunus spinosa, Crataegus laevigata, Rosa canina, Prunus padus, Sorbus aucuparia, Rhamnus cathartica, Morus nigra, Prunus cerasus. The highest average number of birds feeding in forest belts (4.14 ind./km) was registered in oak-maple-linden dense forest belts, while the lowest number (1.48 ind./km) was recorded in wind-blown maple-ash ones. Maple-linden latticed forest belts characterize the best index data of α-diversity of birds. In the summer-autumn diet, succulent fruit are the most important: Starling (Sturnus vulgaris) – 11.8% of the total number of birds observed to feed on this food resource, Greenfinch (Chloris chloris) – 11.3%, Chaffinch (Fringilla coelebs) – 9.3%, Song thrush (Turdus philomelos) – 7.3%, Hawfinch (Coccothraustes coccothraustes) – 7.1%, Blackbird (Turdus merula) – 5.4%. 42 species of birds were observed to feed on black elderberry. More than half (51.2%) of the species composition of birds feeding on fruit and berry plants were migratory birds. Consequently, juicy berries are an important food during bird migrations.

Serological Evidence of West Nile and Usutu Viruses Circulation in Domestic and Wild Birds in Wetlands of Mali and Madagascar in 2008

The geographical distribution and impact on animal and human health of both West Nile and Usutu viruses, two flaviviruses of the Japanese encephalitis complex, have been increasing during the past two decades. Both viruses circulate in Europe and Africa within a natural cycle between wild birds and mosquitoes, mainly from the Culex genus. We retrospectively analyzed sera from domestic and wild birds sampled in 2008 in two wetlands, namely the Inner Niger Delta, Mali, and the Lake Alaotra area, Madagascar. Sera were first tested using a commercial ID Screen West Nile Competition Multi-species ELISA kit. Then, positive sera and sera with insufficient volume for testing with ELISA were tested with a Microneutralization Test. In Mali, the observed seroprevalence in domestic birds was 28.5% [24.5; 32.8] 95%CI, 3.1 % [1.8; 5.2] 95%CI, 6.2% [3.4; 10.2] 95%CI and 9.8 % [7.3; 12.8] 95%CI, for West Nile virus (WNV), Usutu virus (USUV), undetermined flavivirus, and WNV/USUV respectively. Regarding domestic birds of Madagascar, the observed seroprevalence was 4.4 % [2.1; 7.9]95%CI for WNV, 0.9% [0.1; 3.1] 95%CI for USUV, 1.3% [0.5; 2.8] 95%CI for undetermined flavivirus, and null for WNV/USUV. Among the 150 wild birds sampled in Madagascar, two fulvous whistling-ducks (Dendrocygna bicolor) were positive for WNV and two for an undetermined flavivirus. One white-faced whistling-duck (Dendrocygna viduata) and one Hottentot teal (Spatula hottentota) were tested positive for USUV. African and European wetlands are linked by wild bird migrations. This first detection of USUV—as well as the confirmed circulation of WNV in domestic birds of two wetlands of Mali and Madagascar—emphasizes the need to improve the surveillance, knowledge of epidemiological patterns, and phylogenetic characteristics of flavivirus in Africa, particularly in areas prone to sustained, intense flavivirus transmission such as wetlands.

Disentangling the role of Africa in the global spread of H5 highly pathogenic avian influenza

The role of Africa in the dynamics of the global spread of a zoonotic and economically-important virus, such as the highly pathogenic avian influenza (HPAI) H5Nx of the Gs/GD lineage, remains unexplored. Here we characterise the spatiotemporal patterns of virus diffusion during three HPAI H5Nx intercontinental epidemic waves and demonstrate that Africa mainly acted as an ecological sink of the HPAI H5Nx viruses. A joint analysis of host dynamics and continuous spatial diffusion indicates that poultry trade as well as wild bird migrations have contributed to the virus spreading into Africa, with West Africa acting as a crucial hotspot for virus introduction and dissemination into the continent. We demonstrate varying paths of avian influenza incursions into Africa as well as virus spread within Africa over time, which reveal that virus expansion is a complex phenomenon, shaped by an intricate interplay between avian host ecology, virus characteristics and environmental variables.