vesicle stability
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2021 ◽  
Vol 592 ◽  
pp. 119936
Author(s):  
Yu Li ◽  
Fei Xu ◽  
Xiang Li ◽  
Si-Ying Chen ◽  
Lin-Yu Huang ◽  
...  

Polymers ◽  
2020 ◽  
Vol 12 (4) ◽  
pp. 914
Author(s):  
Rashmi Seneviratne ◽  
Lars J. C. Jeuken ◽  
Michael Rappolt ◽  
Paul A. Beales

Sterilisation and preservation of vesicle formulations are important considerations for their viable manufacture for industry applications, particular those intended for medicinal use. Here, we undertake an initial investigation of the stability of hybrid lipid-block copolymer vesicles to common sterilisation and preservation processes, with particular interest in how the block copolymer component might tune vesicle stability. We investigate two sizes of polybutadiene-block-poly(ethylene oxide) polymers (PBd12-PEO11 and PBd22-PEO14) mixed with the phospholipid 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) considering the encapsulation stability of a fluorescent cargo and the colloidal stability of vesicle size distributions. We find that autoclaving and lyophilisation cause complete loss of encapsulation stability under the conditions studied here. Filtering through 200 nm pores appears to be viable for sterilisation for all vesicle compositions with comparatively low release of encapsulated cargo, even for vesicle size distributions which extend beyond the 200 nm filter pore size. Freeze-thaw of vesicles also shows promise for the preservation of hybrid vesicles with high block copolymer content. We discuss the process stability of hybrid vesicles in terms of the complex mechanical interplay between bending resistance, stretching elasticity and lysis strain of these membranes and propose strategies for future work to further enhance the process stability of these vesicle formulations.


Author(s):  
Rashmi Seneviratne ◽  
Lars J.C. Jeuken ◽  
Michael Rappolt ◽  
Paul A. Beales

Sterilisation and preservation of vesicle formulations are an important consideration for their viable manufacture for industry applications, particular those intended for medicinal use. Here we undertake an initial investigation of the stability of hybrid lipid – block copolymer vesicles to common sterilisation and preservation processes, with particular interest in how the block copolymer component might tune vesicle stability. We investigate two sizes of polybutadiene-block-poly(ethylene oxide) polymers (PBd12-PEO11 and PBd22-PEO14) mixed with the phospholipid POPC considering the encapsulation stability of a fluorescent cargo and the colloidal stability of vesicle size distributions. We find that autoclaving and lyophilisation cause complete loss of encapsulation stability under the conditions studied here. Filtering through 200 nm pores appears to be viable for sterilisation for all vesicle compositions with comparatively low release of encapsulated cargo, even for vesicle size distributions which extend beyond the 200 nm filter pore size. Freeze-thaw of vesicles also shows promise for preservation of hybrid vesicles with high block copolymer content. We discuss the process stability of hybrid vesicles in terms of the complex mechanical interplay between bending resistance, stretching elasticity and lysis strain of these membranes and propose strategies for future work to further enhance the process stability of these vesicle formulations.


2019 ◽  
Vol 133 ◽  
pp. S1107
Author(s):  
S. Chin ◽  
A. McWilliam ◽  
D. Brand ◽  
S. Barton ◽  
Y.P. Song ◽  
...  

PLoS ONE ◽  
2018 ◽  
Vol 13 (2) ◽  
pp. e0192422 ◽  
Author(s):  
Maria del Carmen Martínez-Ballesta ◽  
Pablo García-Gomez ◽  
Lucía Yepes-Molina ◽  
Angel L. Guarnizo ◽  
José A. Teruel ◽  
...  

2016 ◽  
Vol 28 (1) ◽  
pp. 22-34 ◽  
Author(s):  
Stephan Holzschuh ◽  
Kathrin Kaeß ◽  
Guilherme Volpe Bossa ◽  
Christiane Decker ◽  
Alfred Fahr ◽  
...  

Life ◽  
2014 ◽  
Vol 4 (4) ◽  
pp. 929-943 ◽  
Author(s):  
Fabrizio Anella ◽  
Christophe Danelon
Keyword(s):  

2014 ◽  
Vol 91 (8) ◽  
pp. 1228-1231 ◽  
Author(s):  
Cristina Del Bianco ◽  
Domenica Torino ◽  
Sheref S. Mansy
Keyword(s):  

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