Hepatic Lipid Peroxide Profile on Inflamation Rats with Nanocurcuminoid Treatment

Inflammation can cause the formation of prostaglandin and free radicals. In addition, overcome inflammation, a drug used also needs to have an effect as antioxidants to treat the free radicals. This problem can be solved by curcuminoid nanoparticles covered with palmitic acid. This research aims to test the antioxidants effect of curcuminoid nanoparticles covered with palmitic acid from Curcuma to lipid peroxide levels on inflammation rats liver. Antioxidants effect of nanocurcuminoid tested on rats that induced by 1% carrageenan and the liver lipid peroxide measured after 24 hours. Lipid peroxide level was measured with a spectrophotometer in 532 nm wavelength. The average size of curcuminoid nanoparticle covered with palmitic acid is 561.53 nm with an IP score of 0.309. Lipid peroxide level of inflammation rats liver given curcuminoids extract 100 mg/kg BW is 0.27x10-4 nmol/g. It was lower from the nanocurcuminoids 250 mg/kg BW groups which are 1.22 x10-4 nmol/g. Nanocurcuminoids 250 mg/kg BW is 114 times more efficient as an antioxidant than curcuminoids extract 100 mg/kg BW. Keywords: antioxidant, lipid peroxide, nanocurcuminoids

Effect of pre-exposure to beta rays of tritium on some biochemical parameters measured in organs of rats subsequently irradiated with fast neutrons

The experiment examined biological responses produced by combined sequential exposure to low-level tritium contamination, followed by challenging irradiation with fast neutrons. Modifications of endogenous antioxidant potential of different organs in rats were discussed in relation to tissue radiosensitivity. Rats pre-contaminated to 7 cGy and 35 cGy have been additionally irradiated to 1 Gy with fast neutrons. Lipid peroxide level was determined in liver, kidney, small intestine, spleen, bone marrow, and plasma. Reduced glutathione (GSH) level and glucose-6-phosphate dehydrogenase (G6PDH) activity were determined in erythrocytes. An in vitro thymidine uptake assay was performed in isolated bone marrow cells. The lipid peroxide level decreased significantly only in liver and kidney from rats pre-exposed to 35 cGy. For small intestine and spleen, tissues of comparatively higher radiosensitivity, no induced radioprotection was observed, as reflected in the homeostasis of the lipid peroxides. The same behavior was observed in bone marrow, the most radiosensitive tissue studied. However, the bone marrow thymidine-incorporation assay revealed a possible adaptive-type reaction in rats pre-exposed to 35 cGy. We conclude that for radiosensitive tissues pre-exposure to chronic low doses of low linear energy transfer (LET) irradiation has no protective effect on their antioxidant status, whereas a protective effect is observed in radioresistent tissues.Key words: induced radioprotection, tritium contamination, lipid peroxides, thymidine uptake, tissue radiosensitivity, antioxidant defense.