Poplar Tree Response to Feeding by the Petiole Gall Aphid Pemphigus spyrothecae Pass

Pemphigus spyrothecae Pass. which is a member of the subfamily Pemphiginae is one of the gall-inducing aphids that occurs on poplar trees. Phloem feeding of a founding mother on leaf petiole results in the formation of a new organ, i.e., the spiral gall. This study documents aphid development inside the galls during the growing season and the effect of their feeding on leaf architecture and physiology of the host plant. In particular, leaf length, width, and area were measured, as well as hydrogen peroxide (H2O2) content, electrolyte leakage (EL), malondialdehyde (MDA) concentration, and the activity of ascorbate (APX) and guaiacol peroxidase (GPX) were determined in galls and galled leaves. The presence of petiole galls significantly decreased the length, width, and leaf area. Aphid activity increased H2O2 concentration in galls and EL from galls and leaf tissues, which was accompanied by a strong decrease in MDA content and both peroxidase activities, especially in gall tissues. It can be suggested that P. spyrothecae can manipulate physiological machinery of the host plant for its own benefit.

QUANTITATIVE ESTIMATION OF THE INFLUENCE OF GALLA AHES OF THE GENUS PEMPHIGUS (STERNORRHYNCHA: APHIDIDAE, PEMPHIGINI) ON THE PARAMETERS OF POPLAR LEAVES

The paper on example of the aphid Pemphigus spyrothecae, features of the formation of galls on the black poplar (Populus nigra) were studied. It was found that the flow of nutrients to the galls is directed both from the side of the shoot, and the leaf on which they are formed.

Occurrence, development and natural enemies of Pemphigus spyrothecae (Homoptera, Pemphigidae)

In 2001, galls were analysed of Pemphigus spyrothecae Pass. taken in one- to three-week intervals from Populus nigra and P. nigra var. italica at 4 localities in Brno. Fundatrices matured in the first half of June and during the first half of summer produced about 50 offsprings. Virgines produced about 10 offsprings which grew up in winged sexuparae. The winged individuals started to occur in galls from the beginning of August. In galls with intact development, on average 500 aphids developed. Galls with intact development on P. nigra var. italica reached larger average dimensions and contained at least by 4% more aphids than galls on P. nigra. About 5% of fundatrices died already in the 1st instar and other 3 to 6% in higher instars by the beginning of reproduction. At localities under investigation, 7.5 to 39.0% of galls on P. nigra and 3.9 to 13.7% of galls on P. nigra var. italica were occupied by the fly Leucopis puncticornis Meig. (Chamaemyiidae). About 24.3 to 32.2% of galls on P. nigra and 23.3 to 49.3% of galls on P. nigra var. italica were occupied by the bug Anthocoris minki Dohrn (Anthocoridae). Hover flies Heringia heringi (Zett.) and Pipiza festiva Meig. (Syrphidae) killing aphids in 3.8 to 30.4% of galls on P. nigra and 6.5 to 6.8% of galls on P. nigra var. italica were an important regulator. In August (i.e. at the beginning of the formation of winged sexuparae), the majority of galls opened through primary slit-shaped or oval emergence holes. A part (7.8 to 19.5%) of galls with so far intact development, however, remained closed and all aphids contained in them died. Diseases (particularly mycoses) often participated in the accelerated dying of aphids. The effect of mortality factors on the gall size differentiation was evaluated in details. The galls do not cause any leaf area reduction. In the case of mass outbreak, they decrease decorativeness of poplars in street alleys. In August and September, liquid excrements fall out from the galls (honeydew) polluting the environment in villages and housing estates.

Molecular Characterization of the Leucine Cluster in Buchnera sp. Strain PSY, a Primary Endosymbiont of the Aphid Pemphigus spyrothecae

ABSTRACT Buchnera strains from most aphid subfamilies studied to date have been found to carry the leucine gene cluster (leuA, -B, -C, and -D) on a plasmid, an organization unique among bacteria. Here, however, we demonstrate a classical chromosomal location of the cluster in Buchnera sp. strain PSY from the aphid Pemphigus spyrothecae (subfamily Pemphiginae). The genes that flank leuABCD in Buchnera sp. strain PSY appear to be adjacent in the genome of Buchnera sp. strain APS, a strain carrying a leucine plasmid. We propose that the presence of a leucine plasmid predates the diversification of symbiotic Buchnera and that the chromosomal location observed in Buchnera sp. strain PSY arose by a transfer of the leucine genes from a plasmid to the chromosome.