Ultracytochemical Observations on Intracytoplasmic Lipids by Enzymic Digestive Methods: Histochemical Trials

The penetration of Rbizopus arrhizus lipase into ethioine fatty livers was examined histochemically. A free-floating, 30 μm thick Vibratome section of the livers perfused with aldehydes was shown to be suitable for ultracytochemical analysis by the lipase digestive method. Accessibility of phospholipids in the outer membrane of erythrocytes to Naja naja phospholipase A2 was observed under a light microscope, and intact red cells were used for the ultracytochemical analysis by the phospholipase digestive method. Ultracytochemical pictures of cytoplasmic lipids in these materials subjected to the enzymatic digestive method are presented.