Combined visualization of central catecholamine-and acetylcholinesterase-containing neurons: Application of the glyoxylic acid and thiocholine histochemical methods to the same Vibratome section

Olle Lindvall

doi:10.1007/bf00491066

Combined visualization of central catecholamine-and acetylcholinesterase-containing neurons: Application of the glyoxylic acid and thiocholine histochemical methods to the same Vibratome section

Histochemistry ◽

10.1007/bf00491066 ◽

1977 ◽

Vol 50 (3) ◽

pp. 191-196 ◽

Cited By ~ 2

Author(s):

Olle Lindvall

Keyword(s):

Glyoxylic Acid ◽

Vibratome Section ◽

Histochemical Methods

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New aspects on factors determining the sensitivity of the formaldehyde and glyoxylic acid fluorescence histochemical methods for monoamines

Histochemistry ◽

10.1007/bf00489512 ◽

1980 ◽

Vol 68 (2) ◽

pp. 169-181 ◽

Cited By ~ 8

Author(s):

O. Lindvall ◽

A. Bj�rklund ◽

B. Falck ◽

I. Lor�n

Keyword(s):

Glyoxylic Acid ◽

Histochemical Methods

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Lemon Juice Mediated Synthesis of 3-Substituted Quinazolin-4(3H)-Ones and their Pharmacological Evaluation

Anti-Cancer Agents in Medicinal Chemistry ◽

10.2174/1871520619666190723151909 ◽

2020 ◽

Vol 19 (16) ◽

pp. 2001-2009 ◽

Cited By ~ 1

Author(s):

Malavattu G. Prasad ◽

C. Vijaya Lakshmi ◽

Naresh K. Katari ◽

Sreekantha B. Jonnalagadda ◽

Manojit Pal

Keyword(s):

Cell Line ◽

Cell Lines ◽

Hek293 Cell ◽

Glyoxylic Acid ◽

Lemon Juice ◽

Cytotoxic Agents ◽

Diverse Range ◽

Hek293 Cell Line ◽

Three Component Reaction ◽

Mcf 7

Background: Compounds containing the quinazoline-4(3H)-one framework constitute an important class of fused N-heterocycles that are found in more than 200 naturally occurring alkaloids. These compounds also show a diverse range of pharmacological activities including antitumor properties. This prompted us to explore a series of quinazolin-4-(3H)-one derivatives having no substituent at C-2 as potential cytotoxic agents. Objective: The objective of this study was to synthesize and evaluate 3-substituted quinazolin-4(3H)-one derivatives for their potential cytotoxic properties. Methods: A convenient method has been developed for the rapid synthesis of this class of compounds under a mild and non-hazardous reaction condition in good yields. The methodology involved a three-component reaction employing isatoic anhydride, amines and glyoxylic acid as reactants in the presence of lemon juice in PEG- 400 at room temperature (25-30ºC) under ultrasound irradiation. All the synthesized compounds were screened via an MTT assay for their potential cytotoxic properties in vitro using the cancerous cell lines e.g. A549, A2780, HepG2, K562, MCF-7 and HCT-116 and a non-cancerous HEK293 cell line. Results: Several compounds such as 3a, 3b, 3d, 3e and 3f showed promising growth inhibition against these cancer cell lines but no significant effects on HEK293 cell line. The IC50 values of these compounds were comparable to doxorubicin whereas 3f significantly induced apoptosis in MCF-7 cells that also was comparable to doxorubicin. Conclusion: An ultrasound-assisted MCR facilitated by lemon juice has been developed to synthesize 3- substituted quinazolin-4(3H)-one derivatives that could act as potential anticancer agents.

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