Study on the association of SLC2A9 rs16890979 with gout in 410 Vietnamese individuals

Gout is a common form of inflammatory arthritis caused by crystallization of acid uric in the joints. The development of gout is not only triggered by environmental factors but also by genetic variation of individuals. In this study, the association between the variation SLC2A9 rs16890979 and gout was investigated. Total DNA was extracted from 410 blood samples of 163 gout patients and 247 age-matched healthy controls. Genotypes of SLC2A9 rs16890979 were obtained using PCR-RFLP. Chi-Square test was used to test whether allele distribution of rs16890979 followed Hardy-Weinberg Equilibrium (HWE). Associations of the clinical characteristics between gout patient and control groups were assessed using Mann-Whitney U. Chi-Square test or Fisher’s exact test was used to check four models (additive, recessive, dominant, co-dominant) for association of rs16890979 with gout. The obtained results showed that the allele distribution of SLC2A9 rs16890979 was in accordance with HWE (p > 0.05). Clinical characteristics such as triglyceride and creatinine were significantly different between gout patient and control groups. However, there was no association of rs16890979 with the risk of gout in Vietnamese population. Further study with a larger sample size should be implemented to confirm our results regarding the association of SLC2A9 rs16890979 with gout in the Vietnamese population. This study would help enrich the knowledge about the effects of hereditary factors on gout disease in the Vietnamese population.

Study on the association SLC17A1 rs1165196 with gout in Vietnamese population

Gout is a common form of inflammatory arthritis caused by urate crystals in a joint from high levels of serum uric acid (SUA). The development of gout is not only triggered by environmental factors but also by genetic variants of individuals. Previous studies demonstrated that the genetic association with gout risk varies in different ethnic populations. The aim of this study was to assess the relationship between SLC17A1 rs1165196 and gout. A total of 169 patients with gout and 351 age-matched healthy controls were recruited at the Nguyen Trai hospital in Southern Vietnam for genomic DNA extraction. Genotypes of SLC17A1 rs1165196 were obtained using PCR-RFLP. Chi-Square test ( 16χ"> 2) was used to test whether allele distribution of rs1165196 follows Hardy-Weinberg Equilibrium (HWE). Associations of the clinical characteristics between gout patient and control groups were assessed using Mann-Whitney U. Chi-Square test or Fisher’s exact test was used to check four models (additive, recessive, dominant, co-dominant) for association of rs1165196 with gout. The results showed that SLC17A1 rs1165196 was in accordance with HWE (p>0.05) and the genotype frequencies of AA, AG and GG were 0.57, 0.38 and 0.05, respectively. The most differences between gout patient and control groups were uric acid and hyperuricemia (p=0.000). Other clinical characteristics such as BMI and CRP levels were also significantly different between gout patient and control groups (p<0.05). However, there was no association of SLC17A1 rs1165196 with the risk of gout in Vietnamese population (p>0.05). Further study with larger sample size should be implemented to confirm our results on the association of SLC17A1 rs1165196 and gout in the Vietnamese population.

Improving gout education from patients’ perspectives: a focus group study of Māori and Pākehā people with gout

ABSTRACT INTRODUCTION Gout is a common form of arthritis that is typically managed in primary care. Gout management guidelines emphasise patient education for successful treatment outcomes, but there is limited literature about the educational experiences of people living with gout in New Zealand, particularly for Māori, who have higher gout prevalence and worse gout outcomes than Pākehā. AIM To explore gout patient education in primary care from the perspectives of Māori and Pākehā people with gout. METHODS In total, 69 people with gout were recruited through primary care providers in three locations across New Zealand. Nine semi-structured focus groups were run with Māori and Pākehā participants in separate groups. RESULTS Thematic analysis yielded two themes in relation to gout education: (i) ‘Multiple sources of gout education’; and (ii) ‘Gaps in gout knowledge’. Participants received education from general practitioners, educational resources, family and friends, and their own experiences. Māori participants preferred information to be kanohi-ki-te-kanohi (face-to-face) and with significant others present where necessary. Participants disclosed gaps in gout’s epidemiology and management. Pākehā and Māori participants reported limited understanding of the genetic basis of gout or the biological underpinnings of the condition and its treatments, but learned treatment adherence through experience. DISCUSSION Despite improved gout patient education, knowledge gaps remain and may contribute to poor medication adherence. Gout patient education interventions need to be tailored to culture and incorporate suitable methods of disseminating information about gout management.