Application of Environmental DNA for Monitoring Red Sea Bream Iridovirus at a Fish Farm

Environmental DNA (eDNA) could be applied in monitoring waterborne viruses of aquatic animals. However, there are few data for practical application of eDNA in fish farms for the control of disease outbreaks.

Virus Disinfection and Population Genetics: Toward the Control of Waterborne Virus Diseases by Water Engineering

Purpose of Review Major waterborne viruses comprise numerous variants rather than only a master sequence and form a genetically diverse population. High genetic diversity is advantageous for adaptation to environmental changes because the highly diverse population likely includes variants resistant to an adverse effect. Disinfection is a broadly employed tool to inactivate pathogens, but due to virus evolvability, waterborne viruses may not be inactivated sufficiently in currently applied disinfection conditions. Here, by focusing on virus population genetics, we explore possibility and factor of emergence of disinfection sensitivity change. Recent Findings To test whether virus population obtains disinfection resistance, the evolutionary experiment developed in the field of population genetics has been applied, indicating the change in disinfection sensitivity. It has been also confirmed that the sensitivity of environmental strains is lower than that of laboratory strains. In some of these studies, genetic diversity within a population less sensitive to disinfection is higher. Researches in virus population genetics have shown the contribution of intra-population genetic diversity to virus population phenotype, so disinfection sensitivity change may attribute to the genetic diversity. Summary The research elucidating a relationship between virus evolution and disinfection has only recently begun, but significant information about the relationship has been accumulated. To develop an effective disinfection strategy for the control of waterborne virus spread, we need to clarify whether disinfection practice truly affects virus outbreaks by refining both laboratory and field experiments related to virus evolution in the disinfection-exerted environment.

Current status of major foodborne and waterborne viral infections and their prevention strategies

Advances in diagnostic techniques and their widespread use for infectious agents revealed the considerably high current prevalence of viral agents in foodborne and waterborne diseases. Foodborne and waterborne viruses are indicated to cause not only gastroenteritis and hepatitis but also neurological disorders, respiratory tract diseases, myocarditis, glomerulonephritis and hemorrhagic fever, with a particularly high mortality rate in infants/children and in individuals with immune deficiency. Additionally, due to their resistance to environmental conditions and food processes compared to other microorganisms, elimination of these viruses by heat and high pressure applications, natural antiviral compounds, UV applications and conventional cleaning-disinfection remains difficult even inadequate. In protection from viral infections, vaccine applications together with GMP, GHP and HACCP system approaches in production seem to be the most effective approaches to ensure the minimization of viruses in food environment and in public. In this review article, up-to-date information is presented on the general characteristics and the diseases caused by enterotropic viruses; NoV, AstV, RoV, AdV and hepatotropic viruses; HAV and HEV, with a particularly high worldwide prevalence, as well as their epidemiology, prevention and their control measures.

Preliminary Data Related to the Effect of Climacostol Produced by the Freshwater Ciliate Climacostomum virens on Human Adenovirus

The new epidemiological scenario has so far focused on the environmental circulation of human viral pathogens. Owing to the side effects of chemical disinfectants, there is an increasing need for knowledge on the use of virucidal compounds, especially those of a natural origin. Climacostol is a molecule produced by a freshwater ciliate and it exhibits activity against bacterial and fungal pathogens. We thus also speculated that there might be an effect on viral viability, which has never been tested. To evaluate such activity, we chose human adenovirus (HAdV), which is representative of waterborne viruses. We conducted experiments using HAdV serotype 5, whose titer was determined by infecting HeLa cell cultures. HAdV5 was shown to be sensitive to climacostol at a concentration of 0.0002 mg/mL, with an approximate 3 Log10 reduction when the initial titer of HAdV5 was approximately 104 and 103 TCID50/mL. These preliminary results could be an important starting point for further research aimed at improving the characterization of climacostol activity under different experimental conditions and against various viruses, including enveloped ones (i.e., the coronavirus). The production of climacostol by a protist living in fresh water also suggests a possible application in the activated sludge of wastewater treatment plants.

Study on norovirus contamination of live bivalve molluscs using real-time PCR

Foodborne and waterborne viruses are a major cause of human morbidity. Of them, noroviruses are recognised as the leading causative agents of sporadic infections and epidemic outbreaks of acute gastroenteritis in humans. Contaminated food products and water are the main source of infection with noroviruses. The infection of bivalve molluscs with human pathogenic viruses occurs by faecal contamination in the production coastal waters. In this study, 47 samples of live bivalve molluscs, including 15 samples of cultivated mussels (Mytilus galloprovincialis) and 32 samples of wild mussels (Tapes decussatus), collected from the Bulgarian and Mediterranean coasts, respectively, were submitted to RT-real-time TaqMan PCR to detect the presence of noroviruses genotype GI and GII. Norovirus genotype GII was found in 11 (23.4%) of all the samples tested. A single mollusc sample (2.1%) was positive for both norovirus genotypes. Our results demonstrated that shellfish intended for sale on the Bulgarian market might pose a potential risk for acquiring norovirus infection. Thus, food safety quality control of shellfish by optimised and standardised methods for detection of foodborne viruses, including noroviruses, should be urgently implemented in Bulgaria.