Eragrostis curvula, a Model Species for Diplosporous Apomixis

Eragrostis curvula (Schrad.) Ness is a grass with a particular apomictic embryo sac development called Eragrostis type. Apomixis is a type of asexual reproduction that produces seeds without fertilization in which the resulting progeny is genetically identical to the mother plant and with the potential to fix the hybrid vigour from more than one generation, among other advantages. The absence of meiosis and the occurrence of only two rounds of mitosis instead of three during embryo sac development make this model unique and suitable to be transferred to economically important crops. Throughout this review, we highlight the advances in the knowledge of apomixis in E. curvula using different techniques such as cytoembryology, DNA methylation analyses, small-RNA-seq, RNA-seq, genome assembly, and genotyping by sequencing. The main bulk of evidence points out that apomixis is inherited as a single Mendelian factor, and it is regulated by genetic and epigenetic mechanisms controlled by a complex network. With all this information, we propose a model of the mechanisms involved in diplosporous apomixis in this grass. All the genetic and epigenetic resources generated in E. curvula to study the reproductive mode changed its status from an orphan to a well-characterised species.

Differential Methylation Patterns in Apomictic vs. Sexual Genotypes of the Diplosporous Grass Eragrostis curvula

DNA methylation is an epigenetic mechanism by which a methyl group is added to a cytosine or an adenine. When located in a gene/regulatory sequence it may repress or de-repress genes, depending on the context and species. Eragrostis curvula is an apomictic grass in which facultative genotypes increases the frequency of sexual pistils triggered by epigenetic mechanisms. The aim of the present study was to look for correlations between the reproductive mode and specific methylated genes or genomic regions. To do so, plants with contrasting reproductive modes were investigated through MCSeEd (Methylation Context Sensitive Enzyme ddRad) showing higher levels of DNA methylation in apomictic genotypes. Moreover, an increased proportion of differentially methylated positions over the regulatory regions were observed, suggesting its possible role in regulation of gene expression. Interestingly, the methylation pathway was also found to be self-regulated since two of the main genes (ROS1 and ROS4), involved in de-methylation, were found differentially methylated between genotypes with different reproductive behavior. Moreover, this work allowed us to detect several genes regulated by methylation that were previously found as differentially expressed in the comparisons between apomictic and sexual genotypes, linking DNA methylation to differences in reproductive mode.

Seed longevity and germination in response to changing drought and heat conditions on four populations of the invasive weed African Lovegrass (Eragrostis curvula)

African lovegrass [Eragrostis curvula (Schrad.) Nees] is an invasive weed that is threatening biodiversity around the world and will continue to do so unless its efficient management is achieved. Consequently, laboratory and field-based experiments were performed to analyse several measures of germination to determine the effect of drought stress, radiant heat stress and burial depth and duration (longevity) on E. curvula seeds. This study investigated seeds from four spatially varied populations across Australia: Maffra and Shepparton, Victoria; Tenterfield, New South Wales; and Midvale, Western Australia. Results showed that increasing drought stress reduced and slowed germination for all populations. Maffra (24% vs. 83%) and Shepparton (41% vs. 74%) were reduced at the osmotic potential of ≤-0.4 MPa, whilst Tenterfield (35% vs. 98.6%) and Midvale (32% vs. 91%) were reduced at ≤-0.6 MPa, compared to the mean of all other osmotic potentials. Radiant heat at 100 C significantly reduced and slowed germination compared to 40 C for Tenterfield (62% vs. 100%), Shepparton (15% vs. 89%) and Midvale (41% vs. 100%); whilst Maffra (75% vs. 86%) had consistent germination. For the effect of burial depth and duration (longevity), there was no significant difference across the fourteen-month period, however, the 0 cm burial depth had a significantly lower final germination percentage compared to depths of 3, 5 and 10 cm (24% vs. 55%). Although each trial was conducted independently, their results can be used to help identify efficient control measures to reduce infesting populations. Such measures recommended include using soil moisture monitoring to detect which conditions will promote germination, as germination is encouraged when the osmotic potential is >-0.6 MPa; exposing seeds to radiant heat (>100 C) using methods such as prescribed burning; and limiting soil disturbance over time to reduce seed establishment.

Non-Native Eragrostis curvula Impacts Diversity of Pastures in South-Eastern Australia Even When Native Themeda triandra Remains Co-Dominant

Lowland grassy woodlands in Australia’s south-east face reductions in native plant diversity because of invasion by non-native plants. We compared the relative abundance and diversity of plant species among sites dominated by the native Kangaroo grass (KG) Themeda triandra with sites co-dominated by the non-native African lovegrass (ALG) Eragrostis curvula and KG. We found significant differences in plant species composition depending on the dominant species. Furthermore, our results revealed differences in several diversity parameters such as a lower species richness and forb diversity on sites co-dominated by ALG and KG. This was the case despite the functional similarity of both ALG and KG—both C4 perennial tussock grasses of a similar height. Therefore, our results highlight the critical function of the native KG in maintaining and enhancing the target plant species composition and diversity within these grassy woodlands. Herbivore grazing potentially impacts on the abundance of the dominant grass and forb species in various ways, but its impact likely differs depending on their evolutionary origin. Therefore, disentangling the role of individual herbivore groups (native-, non-native mammals, and invertebrates) on the plant community composition of the lowland grassy woodlands is essential to find appropriate grazing regimes for ALG management in these ecosystems.

Germination biology of four climatically varied populations of the invasive species African lovegrass (Eragrostis curvula)

African lovegrass [Eragrostis curvula (Schrad.) Nees] is a highly invasive C4 perennial grass that threatens global biodiversity. Appropriate management of this species has been hampered by a lack of knowledge concerning its seed ecology, resulting in significant economic and environmental impacts within various environments. Consequently, this study explored the effects of a selection of environmental factors (photoperiod, alternating temperature, pH, and salinity) by analyzing several measures of germination on four geographically distinct populations of E. curvula to assist in its extirpation from infested sites. Seeds were collected in Australia from Maffra and Shepparton, VIC; Tenterfield, NSW; and Midvale, WA. Key results showed that seeds from Maffra (54% vs. 79%), Tenterfield (38% vs. 61%), and Shepparton (34% vs. 71%) had significantly reduced germination in complete darkness compared with an alternating 12-h light and 12-h dark photoperiod, whereas Midvale had consistent germination (91% vs. 99%). Temperatures between 17/7 C reduced germination for Maffra (42% vs. 73%), Tenterfield (34% vs. 55%), and Shepparton (33% vs. 59%) compared with the mean of all other temperature combinations, whereas Midvale had consistent germination. Furthermore, germination for all populations was consistent between pH 4 and 9. For salinity, germination was significantly reduced at ≥100 mM for Maffra (29% vs. 67%), ≥150 mM for Tenterfield (29% vs. 94%) and Shepparton (39.5% vs. 81.5%), and 250 mM for Midvale (39% vs. 82%) compared with the mean of all other concentrations. Although each trial was conducted independently, the data can be used to generate species-targeted management. Such strategies include maintaining high levels of quarantine and hygiene programs to avoid future spread; where practical, applying light-limiting strategies (mulching, tilling, or scraping) for the Maffra, Tenterfield, and Shepparton populations; and maintaining management efforts year-round, as the species can germinate under a wide range of conditions.

Ranking Species for Veld Restoration in Semi-Arid Regions Using Agronomic, Morphological and Chemical Parameters of Selected Grass Species at Different Developmental Stages under Controlled Environment

The establishment of complementary native grass species could be an ideal method of dealing with existing problems of veld degradation and inadequate forage quantity and quality of pastures. A greenhouse experiment was conducted to evaluate the effect of native grasses viz., Anthephora pubescens, Cenchrus ciliaris, Chloris gayana, Dactylis glomerata, Digitaria eriantha, Eragrostis curvula, Festuca arundinacea, Panicum maximum and Themeda triandra. Attributes at different growth stages on agronomy, morphology and chemical composition were checked. Panicum maximum had the broader (p < 0.05) leaves across all growth stages when compared to all other grass species. Festuca arundinacea had highest (p < 0.05) number of tillers than C. ciliaris, C. gayana, D. glomerata, D. eriantha, E. curvula, P. maximum and T. triandra at 2–4-months age. Within each species, all grasses had the highest (p < 0.05) number of leaves at maturity. Chloris gayana, D. glomerata and P. maximum had the highest (p < 0.05) biomass yield when compared to F. arundinacea at the elongation stage. Eragrostis curvula had the highest (p < 0.05) crude protein (CP) values when compared to all other grasses, except for D. glomerata, F. arundinacea and P. maximum at the elongation stage. Panicum maximum and T. triandra had the least (p < 0.05) acid detergent lignin (ADL) values when compared to all other grasses at both vegetative and the elongation stages. In the ranking, C. ciliaris, C. gayana, D. eriantha, E. curvula, P. maximum and A. pubescens outperformed the rest of the grasses on most parameters. With the low crude protein (CP) content of these grasses, protein supplementation is highly crucial for high performing ruminants, especially those animals that graze grasses as their sole diets.

Genes Modulating the Increase in Sexuality in the Facultative Diplosporous Grass Eragrostis curvula under Water Stress Conditions

Eragrostis curvula presents mainly facultative genotypes that reproduce by diplosporous apomixis, retaining a percentage of sexual pistils that increase under drought and other stressful situations, indicating that some regulators activated by stress could be affecting the apomixis/sexual switch. Water stress experiments were performed in order to associate the increase in sexual embryo sacs with the differential expression of genes in a facultative apomictic cultivar using cytoembryology and RNA sequencing. The percentage of sexual embryo sacs increased from 4 to 24% and 501 out of the 201,011 transcripts were differentially expressed (DE) between control and stressed plants. DE transcripts were compared with previous transcriptomes where apomictic and sexual genotypes were contrasted. The results point as candidates to transcripts related to methylation, ubiquitination, hormone and signal transduction pathways, transcription regulation and cell wall biosynthesis, some acting as a general response to stress and some that are specific to the reproductive mode. We suggest that a DNA glycosylase EcROS1-like could be demethylating, thus de-repressing a gene or genes involved in the sexuality pathways. Many of the other DE transcripts could be part of a complex mechanism that regulates apomixis and sexuality in this grass, the ones in the intersection between control/stress and apo/sex being the strongest candidates.

Methane emissions from sheep fed Eragrostis curvula hay substituted with Lespedeza cuneata

Context Reducing emissions of greenhouse gases from livestock production systems is a global research priority. Forages that contain condensed tannins, such as the perennial legume Lespedeza cuneata, may help to reduce ruminant methane (CH4) emissions. Aims The objective of this study was to investigate the effect of feeding different levels of L. cuneata hay on feed intake and enteric CH4 emissions of sheep fed a basal diet of subtropical Eragrostis curvula hay. Methods Four adult ruminally cannulated Dohne Merino wethers with initial bodyweight of 65.5 ± 3.5 kg were used in the experiment in a 4 × 4 Latin square design. The four experimental treatments were E. curvula hay substituted with 0%, 30%, 60% and 90% L. cuneata hay. Each of four experimental periods lasted 27 days, which consisted of a 14-day adaptation period, a 7-day digestibility trial, and a 6-day CH4-measurement period. During the 6-day CH4-measurement period, CH4 emissions were measured continuously over a 24-h period by using an open circuit respiration system. Key results Dry matter intake (DMI, g/kg W0.75) was higher (P &lt; 0.05) for sheep receiving 60% and 90% L. cuneata than 0% and 30% L. cuneata (77.33 and 84.67 g/kg W0.75 vs 62.96 and 62.71 g/kg W0.75). The increase in DMI corresponded with a linear increase in DM digestibility of the experimental treatments from 38% to 45% as the level of L. cuneata substitution increased. Methane yield was not influenced (P &gt; 0.05) by 30% inclusion of L. cuneata (17.6 g CH4/kg DMI) but decreased (P &lt; 0.05) as the inclusion level increased to 60% and 90% (13.8 and 14.3 g CH4/kg DMI). Conclusions Inclusion of L. cuneata hay in a diet based on E. curvula hay improved diet digestibility, and led to increased concentrations of crude protein, neutral detergent fibre and non-fibre carbohydrates. Substituting E. curvula hay with 60% L. cuneata on a DM basis resulted in the greatest reduction in CH4 yield of 21.4% compared with a diet of 100% E. curvula. Implications The results suggest that L. cuneata has the potential to reduce CH4 yield and possibly increase production from sheep by improving diet DM digestibility and through improved DMI.

Characterizing the Spatial Distribution of Eragrostis Curvula (Weeping Lovegrass) in New Jersey (United States of America) Using Logistic Regression

The increasing spread of invasive plants has become a critical driver of global environmental change. Once established, invasive species are often impossible to eradicate. Therefore, predicting the spread has become a key element in fighting invasive species. In this study, we examined the efficiency of a logistic regression model as a tool to identify the spatial occurrence of an invasive plant species. We used Eragrostis curvula (Weeping Lovegrass) as the dependent variable. The independent variables included temperature, precipitation, soil types, and the road network. We randomly selected 68 georeferenced points to test the goodness of fit of the logistic regression model to predict the presence of E. curvula. We validated the model by selecting an additional 68 random points. Results showed that the probability to successfully predict the presence of E. Curvula was 82.35%. The overall predictive accuracy of the model for the presence or absence of E. Curvula was 80.88%. Additional tests including the Chi-square test, the Hosmer–Lemeshow (HL) test, and the area under the curve (AUC) values, all indicated that the model was the best fit. Our results showed that E. curvula was associated with the identified variables. This study suggests that the logistic regression model can be a useful tool in the identification of invasive species in New Jersey.