Assessing behavioural and physiological responses of three aquatic invertebrates to tributyltin and atrazine in a multi-species, early warning biomonitoring technology

Recent global events and anthropogenic changes to the natural environment have raised concerns about the quality of drinking water consumed by the public throughout the world. Traditional chemical testing is slow and is not performed for all possible contaminants, necessitating the development of innovative new technology to detect and mitigate threats to human health. The development of a multi-species, early-warning biomonitoring technology, based on behavioural and physiological changes in aquatic organisms, greatly furthers this goal. In this study, changes in movement behaviour and respiration rates were examined in three aquatic species, Daphnia magna, Hyalella azteca and Lumbriculus variegates, exposed to varying concentrations of TBT and atrazine, using digital video analysis and direct oxygen measurement. Different parameters of movement were examined and evaluated for inclusion in a multi-species, early-warning biomonitoring technology and the utility of incorporating these parameters into a model to determine classes and concentrations of various contaminants is discussed. An evaluation of whether or not direct measurement of oxygen consumption rates is feasible and useful for inclusion in a multi-species, early-warning biomonitoring technology is also discussed.

Assessing behavioural and physiological responses of three aquatic invertebrates to tributyltin and atrazine in a multi-species, early warning biomonitoring technology

Recent global events and anthropogenic changes to the natural environment have raised concerns about the quality of drinking water consumed by the public throughout the world. Traditional chemical testing is slow and is not performed for all possible contaminants, necessitating the development of innovative new technology to detect and mitigate threats to human health. The development of a multi-species, early-warning biomonitoring technology, based on behavioural and physiological changes in aquatic organisms, greatly furthers this goal. In this study, changes in movement behaviour and respiration rates were examined in three aquatic species, Daphnia magna, Hyalella azteca and Lumbriculus variegates, exposed to varying concentrations of TBT and atrazine, using digital video analysis and direct oxygen measurement. Different parameters of movement were examined and evaluated for inclusion in a multi-species, early-warning biomonitoring technology and the utility of incorporating these parameters into a model to determine classes and concentrations of various contaminants is discussed. An evaluation of whether or not direct measurement of oxygen consumption rates is feasible and useful for inclusion in a multi-species, early-warning biomonitoring technology is also discussed.

COMPUTER-AIDED PHASE IDENTIFICATION AND FRAME-TO-FRAME ANALYSIS OF ENDODONTIC ASYMMETRIC RECIPROCATION ROTATION: A PRELIMINARY STUDY

To perform a detailed evaluation of reciprocating motion using a computer-aided phase identification and frame-to-frame analysis, continuous rotation at 300 rpm, RECIPROC ALL mode and WAVEONE ALL-mode were recorded with a high-speed camera. Movie files were automatically analyzed with digital video analysis and modeling tool. RECIPROC ALL mode parameters were 186.34°±1.02 at 428.32 rpm ±7.61 and 65.07°±0.93 at 261.06 rpm ± 7.72; WAVEONE ALL-mode parameters were 191.39°±1.32 at 523.83 rpm ±14.36 and 70.13°±1.26 at 316.06 rpm ± 8.75. The variability of rotational speed during the cycle and distinct acceleration –deceleration patterns, was similar for both reciprocating modes. The computer-aided frame-to-frame analysis revealed that asymmetrical reciprocating motion has more complex kinematics demonstrating high peak rotational speed values and different patterns of acceleration and deceleration. While there was a difference in reciprocating cycle duration and rotational speed, both cycles demonstrated a similar dynamic of rotational speed during the cycle.

Research Object Rotational Motion Estimation Based on Digital Video Analysis Technique

This paper is about the object rotational motion estimation based on optical flow equation. Being a non-touch measuring technique, it is of important value in some special occasions. It is to set up rigid motion equations by optical flow character, and then using two-step iterative method to estimate motion equation and calculate rotation speed for each coordinate axis. We present a simplified calculation method for some object which specific structure parameters are known. The experiment results show the calculation is accurate.